Comparative performance of WANTAI ELISA for total immunoglobulin to receptor binding protein and an ELISA for IgG to spike protein in detecting SARS-CoV-2 antibodies in Kenyan populations.
Nyagwange, James;
Kutima, Bernadette;
Mwai, Kennedy;
Karanja, Henry K;
Gitonga, John N;
Mugo, Daisy;
Uyoga, Sophie;
Tuju, James;
Ochola-Oyier, Lynette I;
Ndungu, Francis;
+5 more...Bejon, Philip;
Agweyu, Ambrose;
Adetifa, Ifedayo MO;
Scott, J Anthony G;
Warimwe, George M;
(2022)
Comparative performance of WANTAI ELISA for total immunoglobulin to receptor binding protein and an ELISA for IgG to spike protein in detecting SARS-CoV-2 antibodies in Kenyan populations.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 146.
105061-.
ISSN 1386-6532
DOI: https://doi.org/10.1016/j.jcv.2021.105061
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Many SARS-CoV-2 antibody detection assays have been developed but their differential performance is not well described. In this study we compared an in-house (KWTRP) ELISA which has been used extensively to estimate seroprevalence in the Kenyan population with WANTAI, an ELISA which has been approved for widespread use by the WHO. Using a wide variety of sample sets including pre-pandemic samples (negative gold standard), SARS-CoV-2 PCR positive samples (positive gold standard) and COVID-19 test samples from different periods (unknowns), we compared performance characteristics of the two assays. The overall concordance between WANTAI and KWTRP was 0.97 (95% CI, 0.95-0.98). For WANTAI and KWTRP, sensitivity was 0.95 (95% CI 0.90-0.98) and 0.93 (95% CI 0.87-0.96), respectively. Specificity for WANTAI was 0.98 (95% CI, 0.96-0.99) and 0.99 (95% CI 0.96-1.00) while KWTRP specificity was 0.99 (95% CI, 0.98-1.00) and 1.00 using pre-pandemic blood donors and pre-pandemic malaria cross-sectional survey samples respectively. Both assays show excellent characteristics to detect SARS-CoV-2 antibodies.
