Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.
Hasan, Mohammad R;
Kalikiri, Mahesh KR;
Mirza, Faheem;
Sundararaju, Sathyavathi;
Sharma, Anju;
Xaba, Thabisile;
Lorenz, Stephan;
Chemaitelly, Hiam;
El-Kahlout, Reham A;
Tsui, Kin Ming;
+8 more...Yassine, Hadi M;
Coyle, Peter V;
Al Khal, Abdullatif;
Bertollini, Roberto;
Al Thani, Mohamed H;
Abu-Raddad, Laith J;
Tang, Patrick;
National Study Group for COVID-19 Epidemiology in Qatar;
National Study Group for COVID-19 Epidemiology in Qatar;
(2021)
Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 112.
pp. 52-54.
ISSN 1201-9712
DOI: https://doi.org/10.1016/j.ijid.2021.09.006
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Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
