Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi.

Maisa da Silva Araujo ORCID logo ; Alice Oliveira Andrade ; Najara Akira Costa Dos Santos ; Dhélio Batista Pereira ; Glaucilene da Silva Costa ; Paula Frassinetti Medeiros de Paulo ; Carlos Tong Rios ; Marta Moreno ; Luiz Hidelbrando Pereira-da-Silva ; Jansen Fernandes de Medeiros ; (2019) Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi. REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 52. e20190159-. ISSN 0037-8682 DOI: 10.1590/0037-8682-0159-2019
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INTRODUCTION: The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil. METHODS: Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation. RESULTS: In 11 generations, the colony produced a high number of pupae after the F5 generation. CONCLUSIONS: These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.


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