Greenwood, BM; Greenwood, AM; Bradley, AK; Shenton, FC; Smith, AW; Snow, RW; Williams, K; Eggelte, TA; Huikeshoven, H; de Wit, M; (1986) ELISA tests for dapsone and pyrimethamine and their application in a malaria chemoprophylaxis programme. Bulletin of the World Health Organization, 64 (6). pp. 909-916. ISSN 0042-9686 https://researchonline.lshtm.ac.uk/id/eprint/4654020
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https://researchonline.lshtm.ac.uk/id/eprint/4654020
Abstract
Enzyme-linked immunosorbent asays (ELISAs) are described for determining levels of dapsone and pyrimethamine in urine. Both assays have a sensitivity of about 20 mug/l and are reproducible, but each produces some false positives. The problem of false positive reactions was partially obviated by requiring positive results in both assays. In a pilot study involving 50 children aged 3 months to 4 years who were given a single dose of Maloprim (pyrimethamine + dapsone), 75% were positive for dapsone 7 days after administration of the drug, while 25% were still positive 15 days after its administration. The corresponding proportions for pyrimethamine were 73% and 30%, respectively. Comparison of the results obtained in a larger chemoprophylaxis trial with those from the pilot study indicated that the assays described could be used to investigate whether antimalarials had been taken.
Item Type | Article |
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Faculty and Department | Faculty of Infectious and Tropical Diseases > Dept of Disease Control |
PubMed ID | 3493860 |
Elements ID | 133159 |