Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
Larrouy-Maumus, G; Clements, A; Filloux, A; McCarthy, RR; Mostowy, S
and
(2016)
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
JOURNAL OF MICROBIOLOGICAL METHODS, 120.
pp. 68-71.
ISSN 0167-7012
DOI: 10.1016/j.mimet.2015.12.004
and
(2016)
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
JOURNAL OF MICROBIOLOGICAL METHODS, 120.
pp. 68-71.
ISSN 0167-7012
DOI: 10.1016/j.mimet.2015.12.004
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
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