A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia.
Nguyen
Van Hong
;
Peter
van den Eede
;
Chantal
Van Overmeir
;
Indra
Vythilingham
;
Anna
Rosanas-Urgell
;
Pham
Vinh Thanh
;
Ngo Duc
Thang
;
Nguyen Manh
Hung
;
Le Xuan
Hung
;
Umberto
D'Alessandro
;
+1 more...
Annette
Erhart
;
(2013)
A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 89 (4).
pp. 721-723.
ISSN 0002-9637
DOI: 10.4269/ajtmh.13-0027
;
+1 more...
Annette
Erhart
;
(2013)
A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 89 (4).
pp. 721-723.
ISSN 0002-9637
DOI: 10.4269/ajtmh.13-0027
We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.
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