Direct evidence that asparagine at position 108 of the Plasmodium falciparum dihydrofolate reductase is involved in resistance to antifolate drugs in Tanzania

Curtis, J; Duraisingh, MT; Trigg, JK; Mbwana, H; Warhurst, DC; Curtis, CF; (1996) Direct evidence that asparagine at position 108 of the Plasmodium falciparum dihydrofolate reductase is involved in resistance to antifolate drugs in Tanzania. Transactions of the Royal Society of Tropical Medicine and Hygiene, 90 (6). pp. 678-80. ISSN 0035-9203 https://researchonline.lshtm.ac.uk/id/eprint/4649875

Abstract

A nested polymerase chain reaction was used to amplify a fragment of the gene for dihydrofolate reductase of Plasmodium falciparum containing codon 108, where a point mutation, causing a serine to asparagine change, occurs in pyrimethamine resistant parasites. The presence of the mutation was detected by restriction enzyme digestion. Parasites in blood samples collected from asymptomatic children before, and 3 weeks after, treatment with pyrimethamine-sulfadoxine or chlorproguanil-dapsone were analysed. Parasites in the samples taken at 3 weeks carried only the asparagine mutant.

Additional Information

Item Type	Article
Keywords	Animals, Child, Child, Preschool, Drug Resistance/genetics, Folic Acid Antagonists/therapeutic use, Humans, Infant, Plasmodium falciparum/genetics, Point Mutation, Polymerase Chain Reaction, Tetrahydrofolate Dehydrogenase/*genetics
Faculty and Department	Faculty of Infectious and Tropical Diseases > Department of Infection Biology > Dept of Pathogen Molecular Biology (-2019)