Kumkate, Supeecha; Jenkins, Gavin R; Paveley, Ross A; Hogg, Karen G; Mountford, Adrian P; (2007) CD207+ Langerhans cells constitute a minor population of skin-derived antigen-presenting cells in the draining lymph node following exposure to Schistosoma mansoni. International journal for parasitology, 37 (2). pp. 209-220. ISSN 0020-7519 DOI: https://doi.org/10.1016/j.ijpara.2006.10.007
Permanent Identifier
Use this Digital Object Identifier when citing or linking to this resource.
Abstract
Infectious cercariae of Schistosoma mansoni gain entry to the mammalian host through the skin where they induce a transient inflammatory influx of mononuclear cells. Some of these cells have antigen-presenting cell function (MHCII+) and have been reported to migrate to the skin-draining lymph nodes (sdLN) where they have the potential to prime CD4+ cells of the acquired immune response. Here, in mice exposed to vaccinating radiation-attenuated schistosome larvae, which induce high levels of protective immunity to challenge infection, we describe the parasite-induced migration of Langerhans cells (LCs) from the epidermal site of immunisation to the sdLN using a specific monoclonal antibody that recognises langerin (CD207). CD207+ cells with dendritic morphology were abundant in the epidermis at all times and their migration into the dermis was detected soon after vaccination. All CD207+ LCs were MHCII+ but not all MHCII+ cells in the skin were CD207+. LCs migrated from the dermis in enhanced numbers after vaccination, as detected in dermal exudate populations recovered after in vitro culture of skin biopsies. Elevated numbers of CD207+ LCs were also detected in the sdLN from 24h to 4 days after vaccination. However, compared with other dermal-derived antigen-presenting cells that were CD207-MHCII+ or CD207-CD11c+, the relative numbers of CD207+ cells in the dermal exudate population and in the sdLN were very small. Furthermore, the migration of CD207+ cells after exposure to 'protective' radiation-attenuated, compared with 'non-protective' normal cercariae, was similar in terms of numbers and kinetics. Together, these studies suggest that CD207+ LCs are only a minor component of the antigen-presenting cell population that migrates from the epidermis and they are unlikely to be important in the priming of protective CD4+ cells in the sdLN.
Item Type | Article |
---|---|
Keywords | CD207, Helminth, Langerhans cell, Schistosoma, Vaccine, Animals, Antibodies, Monoclonal, pharmacology, Antigens, Surface, immunology, Dermis, cytology, Epidermis, cytology, Female, Immunophenotyping, Langerhans Cells, immunology, Lectins, C-Type, immunology, Lymph Nodes, immunology, Mannose-Binding Lectins, immunology, Mice, Mice, Inbred C57BL, Schistosoma mansoni, immunology, Schistosomiasis mansoni, immunology |
Faculty and Department | Faculty of Infectious and Tropical Diseases > Department of Infection Biology > Dept of Immunology and Infection (-2019) |
PubMed ID | 17157855 |
ISI | 244164500009 |
Related URLs |