From FASTQ to Function: In Silico Methods for Processing Next-Generation Sequencing Data.
Preston, MD;
Stabler, RA;
(2016)
From FASTQ to Function: In Silico Methods for Processing Next-Generation Sequencing Data.
In: Roberts, AP; Mullany, P, (eds.)
Clostridium difficile.
Springer, New York, pp. 23-33.
ISBN 9781493963591
DOI: https://doi.org/10.1007/978-1-4939-6361-4_3
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This chapter presents a method to process C. difficile whole-genome next-generation sequencing data straight from the sequencer. Quality control processing and de novo assembly of these data enable downstream analyses such as gene annotation and in silico multi-locus strain-type identification.
