Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
William P
Heal
;
Sasala R
Wickramasinghe
;
Paul W
Bowyer
;
Anthony A
Holder
;
Deborah F
Smith
;
Robin J
Leatherbarrow
;
Edward W
Tate
;
(2008)
Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
Chemical communications (Cambridge, England) (4).
pp. 480-482.
ISSN 1359-7345
DOI: 10.1039/b716115h
N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.
Item Type | Article |
---|---|
ISI | 252254500017 |
Date Deposited | 03 Jun 2013 12:17 |