Molecular analysis of carbohydrate-antibody interactions: case study using a Bacillus anthracis tetrasaccharide.
Matthias A
Oberli
;
Marco
Tamborrini
;
Yu-Hsuan
Tsai
;
Daniel B
Werz
;
Tim
Horlacher
;
Alexander
Adibekian
;
Dominik
Gauss
;
Heiko M
Möller
;
Gerd
Pluschke
;
Peter H
Seeberger
;
(2010)
Molecular analysis of carbohydrate-antibody interactions: case study using a Bacillus anthracis tetrasaccharide.
Journal of the American Chemical Society, 132 (30).
pp. 10239-10241.
ISSN 1520-5126
DOI: 10.1021/ja104027w
The process for selecting potent and effective carbohydrate antigens is not well-established. A combination of synthetic glycan microarray screening, surface plasmon resonance analysis, and saturation transfer difference NMR spectroscopy was used to dissect the antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-based design of carbohydrate antigens.
| Item Type | Article |
|---|---|
| Date Deposited | 15 Dec 2011 14:17 |