Molecular analysis of carbohydrate-antibody interactions: case study using a Bacillus anthracis tetrasaccharide.
Oberli, Matthias A;
Tamborrini, Marco;
Tsai, Yu-Hsuan;
Werz, Daniel B;
Horlacher, Tim;
Adibekian, Alexander;
Gauss, Dominik;
Möller, Heiko M;
Pluschke, Gerd;
Seeberger, Peter H;
(2010)
Molecular analysis of carbohydrate-antibody interactions: case study using a Bacillus anthracis tetrasaccharide.
Journal of the American Chemical Society, 132 (30).
pp. 10239-10241.
ISSN 1520-5126
DOI: https://doi.org/10.1021/ja104027w
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The process for selecting potent and effective carbohydrate antigens is not well-established. A combination of synthetic glycan microarray screening, surface plasmon resonance analysis, and saturation transfer difference NMR spectroscopy was used to dissect the antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-based design of carbohydrate antigens.
