Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
Bossé, JT; Li, Y; Angen, Ø; Weinert, LA; Chaudhuri, RR; Holden, MT; Williamson, SM; Maskell, DJ; Tucker, AW; Wren, BW
; +3 more...Rycroft, AN; Langford, PR; BRaDP1T consortium and
(2014)
Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
Journal of clinical microbiology, 52 (7).
pp. 2380-2385.
ISSN 0095-1137
DOI: 10.1128/JCM.00685-14
An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
Item Type | Article |
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ISI | 339279700015 |
Date Deposited | 29 Apr 2014 19:43 |
Explore Further
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097740 (OA Location)
- 10.1128/JCM.00685-14 (DOI)
- 24759717 (PubMed)
ORCID: https://orcid.org/0000-0002-6140-9489