Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
Janine T
Bossé
;
Yanwen
Li
;
Øystein
Angen
;
Lucy A
Weinert
;
Roy R
Chaudhuri
;
Matthew T
Holden
;
Susanna M
Williamson
;
Duncan J
Maskell
;
Alexander W
Tucker
;
Brendan W
Wren
;
+3 more...
Andrew N
Rycroft
;
Paul R
Langford
;
BRaDP1T consortium
;
(2014)
Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
Journal of clinical microbiology, 52 (7).
pp. 2380-2385.
ISSN 0095-1137
DOI: 10.1128/JCM.00685-14
;
+3 more...
Andrew N
Rycroft
;
Paul R
Langford
;
BRaDP1T consortium
;
(2014)
Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
Journal of clinical microbiology, 52 (7).
pp. 2380-2385.
ISSN 0095-1137
DOI: 10.1128/JCM.00685-14
An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
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picture_as_pdf - zjm2380.pdf
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