Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.

Tanomsing, N; Imwong, M; Sutherland, CJORCID logo; Dolecek, C; Hien, TT; Nosten, F; Day, NP; White, NJ; Snounou, G and (2013) Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri. Journal of clinical microbiology, 51 (12). pp. 4213-4216. ISSN 0095-1137 DOI: 10.1128/JCM.01527-13
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We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.


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