Mycobacterial phosphatidylinositol mannoside is a natural antigen for CD1d-restricted T cells.
Fischer, Karsten;
Scotet, Emmanuel;
Niemeyer, Marcus;
Koebernick, Heidrun;
Zerrahn, Jens;
Maillet, Sophie;
Hurwitz, Robert;
Kursar, Mischo;
Bonneville, Marc;
Kaufmann, Stefan HE;
+1 more...Schaible, Ulrich E;
(2004)
Mycobacterial phosphatidylinositol mannoside is a natural antigen for CD1d-restricted T cells.
Proceedings of the National Academy of Sciences of the United States of America, 101 (29).
pp. 10685-10690.
ISSN 0027-8424
DOI: https://doi.org/10.1073/pnas.0403787101
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A group of T cells recognizes glycolipids presented by molecules of the CD1 family. The CD1d-restricted natural killer T cells (NKT cells) are primarily considered to be self-reactive. By employing CD1d-binding and T cell assays, the following structural parameters for presentation by CD1d were defined for a number of mycobacterial and mammalian lipids: two acyl chains facilitated binding, and a polar head group was essential for T cell recognition. Of the mycobacterial lipids tested, only a phosphatidylinositol mannoside (PIM) fulfilled the requirements for CD1d binding and NKT cell stimulation. This PIM activated human and murine NKT cells via CD1d, thereby triggering antigen-specific IFN-gamma production and cell-mediated cytotoxicity, and PIM-loaded CD1d tetramers identified a subpopulation of murine and human NKT cells. This phospholipid, therefore, represents a mycobacterial antigen recognized by T cells in the context of CD1d.
