IL-4 and T cells are required for the generation of IgG1 isotype antibodies against cardiolipin.
Fischer, Karsten;
Collins, Helen;
Taniguchi, Masaru;
Kaufmann, Stefan HE;
Schaible, Ulrich E;
(2002)
IL-4 and T cells are required for the generation of IgG1 isotype antibodies against cardiolipin.
Journal of immunology (Baltimore, Md, 168 (6).
pp. 2689-2694.
ISSN 0022-1767
DOI: https://doi.org/10.4049/jimmunol.168.6.2689
Permanent Identifier
Use this Digital Object Identifier when citing or linking to this resource.
Infection with Mycobacterium tuberculosis induces Abs against a vast array of mycobacterial lipids and glycolipids. One of the most prominent lipid Ags recognized is cardiolipin (CL). The kinetics of the generation of anti-CL Abs during infection reveals that IgM titers to CL increase over time. Interestingly, at day 30 postinfection CL-specific IgG1 appears, an isotype usually dependent on T cell help. Using an immunization schedule with CL/anti-CL Ab complexes, which induces antiphospholipid syndrome in mice, we show that the generation of IgG1 to CL requires IL-4 and that optimal production is T cell dependent. IgG1 production to CL was impaired in nude (nu/nu) mice devoid in conventional T cells, but was not affected in mice deficient for either alphabeta TCR(+), gammadelta TCR(+), CD4(+), CD8(+), or NK1.1(+) T cells. We conclude that IgG1 production to CL depends on T cell help and IL-4, which can be provided by different T cell populations. This is the first report that IL-4 is indispensable for the induction of IgG1 Abs to lipid Ags.