Wang, Xuan-Yi; Ansaruzzaman, M; Vaz, Raul; Mondlane, Catarina; Lucas, Marcelino ES; von Seidlein, Lorenz; Deen, Jacqueline L; Ampuero, Sonia; Puri, Mahesh; Park, Taesung; +6 more... Nair, GB; Clemens, John D; Chaignat, Claire-Lise; Rajerison, Minoarisoa; Nato, Farida; Fournier, Jean-Michel; (2006) Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population. BMC infectious diseases, 6 (1). 17-. ISSN 1471-2334 DOI: https://doi.org/10.1186/1471-2334-6-17
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Abstract
BACKGROUND: Early detection of cholera outbreaks is crucial for the implementation of the most appropriate control strategies. METHODS: The performance of an immunochromatographic dipstick test (Institute Pasteur, Paris, France) specific for Vibrio cholerae O1 was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season (January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture. RESULTS: The overall sensitivity and specificity of the rapid test compared to culture were 95% (95% confidence interval [CI]: 91%-99%) and 89% (95% CI: 86%-93%), respectively. After stratification by type of sample (rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%. CONCLUSION: This one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed.
Item Type | Article |
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Keywords | Adolescent, Adult, Child, Child, Preschool, Cholera/*diagnosis, Feces/microbiology, Female, Humans, Immunologic Tests/*instrumentation/*methods, Male, Mozambique, Risk, Sensitivity and Specificity, Adolescent, Adult, Child, Child, Preschool, Cholera, diagnosis, Feces, microbiology, Female, Humans, Immunologic Tests, instrumentation, methods, Male, Mozambique, Risk, Sensitivity and Specificity |
Faculty and Department | Faculty of Infectious and Tropical Diseases > Dept of Disease Control |
PubMed ID | 16451731 |
ISI | 236024600001 |
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