%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Marcelino Garrine, Inácio Mandomando, Delfino Vubil, Tacilta Nhampossa, Sozinho Acacio, Shan Li, Joseph N. Paulson, Mathieu Almeida, Daryl Domman, Nicholas R. Thomson, Pedro Alonso, Oscar Colin Stine
Minimal genetic change in Vibrio cholerae in Mozambique over time: Multilocus variable number tandem repeat analysis and whole genome sequencing
www.plosntds.org
www.plosntds.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 14 0 R/Contents 15 0 R/TrimBox[0 0 612 792]>>
endobj
14 0 obj
[16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R]
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref001)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref002)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref003)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref004)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref005)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref006)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref007)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref008)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref009)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref009)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref012)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref013)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref013)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref014)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref013)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref015)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref013)>>
endobj
33 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref016)>>
endobj
34 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref012)>>
endobj
35 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref017)>>
endobj
36 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref015)>>
endobj
37 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref018)>>
endobj
38 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref019)>>
endobj
39 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref020)>>
endobj
40 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref021)>>
endobj
41 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref022)>>
endobj
42 0 obj
<>/Border[0 0 0]/A 43 0 R>>
endobj
43 0 obj
<>
endobj
15 0 obj
[44 0 R 45 0 R 46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R 60 0 R 61 0 R 62 0 R 63 0 R 64 0 R 65 0 R]
endobj
44 0 obj
<>stream
q
0.955 g
0 w
199.9998 713.9999 m
575.9997 713.9999 l
575.9997 611.0002 l
199.9998 611.0002 l
f*
0.83 0.64 0.02 0 k
478.3748 536.9386 m
483.137 536.9386 l
h
f*
538.4693 484.8945 m
543.2315 484.8945 l
h
f*
541.5874 471.9118 m
546.3496 471.9118 l
h
f*
319.5213 445.9465 m
324.2835 445.9465 l
h
f*
326.5512 445.9465 m
331.3134 445.9465 l
h
f*
234.9921 432.9071 m
239.7543 432.9071 l
h
f*
357.6756 419.9244 m
362.4378 419.9244 l
h
f*
379.9559 406.9417 m
384.7181 406.9417 l
h
f*
523.8992 393.9024 m
528.6047 393.9024 l
h
f*
519.2504 380.9197 m
524.0126 380.9197 l
h
f*
529.1717 380.9197 m
538.6961 380.9197 l
h
f*
516.5858 354.8976 m
526.1102 354.8976 l
h
f*
203.4142 328.9323 m
212.9386 328.9323 l
h
f*
391.4079 315.9496 m
400.9323 315.9496 l
h
f*
285.789 302.9102 m
295.3134 302.9102 l
h
f*
256.0819 289.9276 m
265.6063 289.9276 l
h
f*
531.0992 263.9055 m
540.6236 263.9055 l
h
f*
542.8913 263.9055 m
552.4157 263.9055 l
h
f*
388.4031 224.9008 m
397.9276 224.9008 l
h
f*
382.7906 198.9354 m
392.2583 198.9354 l
h
f*
322.0724 185.8961 m
331.5402 185.8961 l
h
f*
252.0567 159.9307 m
261.5811 159.9307 l
h
f*
303.4205 146.948 m
312.9449 146.948 l
h
f*
229.2661 107.9433 m
238.7906 107.9433 l
h
f*
384.2079 107.9433 m
393.6756 107.9433 l
h
f*
395.9433 107.9433 m
405.4677 107.9433 l
h
f*
0 g
1 j
1 J
10 0 0 10 209.9905 696.9826 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(to)Tj
1.0261 0 Td
(each)Tj
2.0296 0 Td
(other)Tj
2.3471 0 Td
(based)Tj
2.4717 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(number)Tj
3.4185 0 Td
(and)Tj
1.7291 0 Td
(type)Tj
1.9163 0 Td
(of)Tj
1.0148 0 Td
(DNA)Tj
2.3754 0 Td
(changes.)Tj
3.6283 0 Td
(In)Tj
1.1112 0 Td
(our)Tj
1.627 0 Td
(study,)Tj
2.6136 0 Td
(we)Tj
1.3266 0 Td
(examined)Tj
-31.3793 -1.2982 Td
(the)Tj
1.4683 0 Td
(DNA)Tj
2.3811 0 Td
(of)Tj
1.0148 0 Td
(cholera)Tj
3.1521 0 Td
(isolates)Tj
3.1181 0 Td
(from)Tj
2.2053 0 Td
(southern)Tj
3.7871 0 Td
(Mozambique.)Tj
5.7429 0 Td
(We)Tj
1.6044 0 Td
(were)Tj
2.126 0 Td
(surprised)Tj
3.9458 0 Td
(how)Tj
1.9389 0 Td
(similar)Tj
-32.4848 -1.2983 Td
(all)Tj
1.1451 0 Td
(the)Tj
1.474 0 Td
(Mozambique)Tj
5.5162 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.1203 0 Td
(even)Tj
2.075 0 Td
(though)Tj
3.0727 0 Td
(that)Tj
1.7745 0 Td
(were)Tj
2.126 0 Td
(collected)Tj
3.736 0 Td
(up)Tj
1.2699 0 Td
(to)Tj
1.0205 0 Td
(eight)Tj
2.2053 0 Td
(years)Tj
2.262 0 Td
(apart.)Tj
-32.9156 -1.3039 Td
(Based)Tj
2.5454 0 Td
(on)Tj
1.2756 0 Td
(previous)Tj
3.651 0 Td
(work,)Tj
2.5058 0 Td
(we)Tj
1.3266 0 Td
(would)Tj
2.7099 0 Td
(have)Tj
2.0637 0 Td
(expected)Tj
3.719 0 Td
(much)Tj
2.5228 0 Td
(more)Tj
2.3414 0 Td
(change)Tj
3.0387 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(DNA.)Tj
2.6022 0 Td
(Our)Tj
-32.8079 -1.2983 Td
(data)Tj
1.9162 0 Td
(raises)Tj
2.4377 0 Td
(several)Tj
2.931 0 Td
(important)Tj
4.286 0 Td
(questions)Tj
4.0195 0 Td
(that)Tj
1.7801 0 Td
(relate)Tj
2.4152 0 Td
(to)Tj
1.0261 0 Td
(where)Tj
2.6475 0 Td
(these)Tj
2.2564 0 Td
(cholera)Tj
3.1521 0 Td
(isolates)Tj
3.1181 0 Td
(persist,)Tj
-31.9859 -1.2982 Td
(possibly)Tj
3.4412 0 Td
(in)Tj
1.0375 0 Td
(local)Tj
2.0749 0 Td
(refuges,)Tj
3.3052 0 Td
(and)Tj
1.7291 0 Td
(how)Tj
1.9389 0 Td
(seemingly)Tj
4.2293 0 Td
(identical)Tj
3.6566 0 Td
(isolates)Tj
3.1181 0 Td
(can)Tj
1.6271 0 Td
(be)Tj
1.1509 0 Td
(collected)Tj
3.7417 0 Td
(years)Tj
2.262 0 Td
(apart)Tj
-33.3125 -1.3039 Td
(despite)Tj
3.0387 0 Td
(our)Tj
1.627 0 Td
(understanding)Tj
6.0718 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(high)Tj
2.0069 0 Td
(rate)Tj
1.7461 0 Td
(of)Tj
1.0148 0 Td
(change)Tj
3.0444 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(molecular)Tj
4.2179 0 Td
(clock.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 568.0062 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 550.9984 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Cholera)Tj
3.3902 0 Td
(remains)Tj
3.4469 0 Td
(a)Tj
0.6519 0 Td
(public)Tj
2.7099 0 Td
(health)Tj
2.6759 0 Td
(concern)Tj
3.4696 0 Td
(in)Tj
1.0318 0 Td
(developing)Tj
4.6035 0 Td
(countries)Tj
3.9458 0 Td
(with)Tj
2.0012 0 Td
(an)Tj
1.2019 0 Td
(estimated)Tj
4.0705 0 Td
(burden)Tj
3.1294 0 Td
(of)Tj
-36.3285 -1.2982 Td
(1.24.3)Tj
3.0897 0 Td
(million)Tj
3.1238 0 Td
(cases)Tj
2.2223 0 Td
(and)Tj
1.7291 0 Td
(28,000142,000)Tj
6.4176 0 Td
(deaths)Tj
2.795 0 Td
(per)Tj
1.5307 0 Td
(year,)Tj
2.1316 0 Td
(worldwide)Tj
4.456 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(].)Tj
1.5988 0 Td
(South)Tj
2.5568 0 Td
(Asia)Tj
1.9672 0 Td
(and)Tj
1.7292 0 Td
(sub-)Tj
-35.3478 -1.3039 Td
(Saharan)Tj
3.4412 0 Td
(Africa)Tj
2.6929 0 Td
(account)Tj
3.3789 0 Td
(with)Tj
2.0012 0 Td
(the)Tj
1.4683 0 Td
(majority)Tj
3.6284 0 Td
(of)Tj
1.0148 0 Td
(cases)Tj
2.2223 0 Td
(and)Tj
1.7291 0 Td
(deaths.)Tj
3.0161 0 Td
(Between)Tj
3.6056 0 Td
(01)Tj
1.1679 0 Td
(January)Tj
3.3222 0 Td
(and)Tj
1.7291 0 Td
(03)Tj
1.1735 0 Td
(June)Tj
-35.5915 -1.2983 Td
(2013,)Tj
2.347 0 Td
(a)Tj
0.652 0 Td
(total)Tj
2.0069 0 Td
(of)Tj
1.0148 0 Td
(25,762)Tj
2.8233 0 Td
(cholera)Tj
3.1521 0 Td
(cases)Tj
2.2223 0 Td
(and)Tj
1.7292 0 Td
(490)Tj
1.6497 0 Td
(deaths)Tj
2.7893 0 Td
(were)Tj
2.126 0 Td
(reported)Tj
3.6566 0 Td
(from)Tj
2.2054 0 Td
(18)Tj
1.1735 0 Td
(African)Tj
3.2372 0 Td
(countries.)Tj
-32.7853 -1.2983 Td
(Mozambique)Tj
5.5161 0 Td
(accounted)Tj
4.337 0 Td
(for)Tj
1.3833 0 Td
(7%)Tj
1.4513 0 Td
(\(1,861/25,762\))Tj
5.9641 0 Td
(of)Tj
1.0148 0 Td
(cases)Tj
2.2223 0 Td
(and)Tj
1.7292 0 Td
(4%)Tj
1.4513 0 Td
(\(19/490\))Tj
3.6113 0 Td
(of)Tj
1.0148 0 Td
(deaths,)Tj
3.0161 0 Td
(being)Tj
2.4321 0 Td
(the)Tj
-35.1437 -1.3039 Td
(third)Tj
2.211 0 Td
(most)Tj
2.2053 0 Td
(affected)Tj
3.3279 0 Td
(country)Tj
3.3505 0 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4684 0 Td
(Democratic)Tj
4.9266 0 Td
(Republic)Tj
3.753 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(Congo)Tj
2.914 0 Td
(and)Tj
1.7292 0 Td
(Angola)Tj
3.1011 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(].)Tj
1.6044 0 Td
(The)Tj
-35.1097 -1.2982 Td
(most)Tj
2.2053 0 Td
(recent)Tj
2.7099 0 Td
(cholera)Tj
3.1521 0 Td
(outbreak)Tj
3.7871 0 Td
(in)Tj
1.0318 0 Td
(Mozambique)Tj
5.5218 0 Td
(started)Tj
2.931 0 Td
(in)Tj
1.0375 0 Td
(December)Tj
4.3483 0 Td
(2014)Tj
2.1203 0 Td
(in)Tj
1.0375 0 Td
(Nampula)Tj
3.9345 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(],)Tj
-33.8171 -1.2983 Td
(where)Tj
2.6475 0 Td
(there)Tj
2.2677 0 Td
(were)Tj
2.1203 0 Td
(8,835)Tj
2.3471 0 Td
(cases)Tj
2.2223 0 Td
(with)Tj
2.0013 0 Td
(case)Tj
1.8595 0 Td
(fatality)Tj
2.9423 0 Td
(rate)Tj
1.7462 0 Td
(of)Tj
1.0148 0 Td
(0.7%)Tj
2.1543 0 Td
(\(65)Tj
1.508 0 Td
(deaths\))Tj
3.1351 0 Td
(in)Tj
1.0375 0 Td
(5)Tj
0.6916 0 Td
(northern)Tj
3.8154 0 Td
(and)Tj
1.7348 0 Td
(cen-)Tj
-35.2457 -1.2983 Td
(tral)Tj
1.5647 0 Td
(provinces)Tj
4.0875 0 Td
(in)Tj
1.0318 0 Td
(five)Tj
1.6498 0 Td
(months)Tj
3.2768 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(].)Tj
2.3017 0 Td
(The)Tj
1.7745 0 Td
(most)Tj
2.2053 0 Td
(recent)Tj
2.7043 0 Td
(cholera)Tj
3.1464 0 Td
(outbreak)Tj
3.7814 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4683 0 Td
(south)Tj
2.4378 0 Td
(was)Tj
1.6951 0 Td
(reported)Tj
-34.1572 -1.3039 Td
(in)Tj
1.0318 0 Td
(2011)Tj
2.1259 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(].)Tj
1.5988 0 Td
(The)Tj
1.7858 0 Td
(peak)Tj
2.0919 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(cholera)Tj
3.1521 0 Td
(epidemic)Tj
3.8891 0 Td
(is)Tj
0.8504 0 Td
(often)Tj
2.2847 0 Td
(preceded)Tj
3.8551 0 Td
(by)Tj
1.1849 0 Td
(increasing)Tj
4.3313 0 Td
(prevalence)Tj
4.4844 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-35.3478 -1.2983 Td
(pathogenic)Tj
4.6317 0 Td
(strains)Tj
2.863 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4683 0 Td
(environment)Tj
5.4312 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(])Tj
1.3776 0 Td
(where)Tj
/F9 1 Tf
2.6532 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1055 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(are)Tj
1.4514 0 Td
(harbored)Tj
3.8891 0 Td
(in)Tj
1.0375 0 Td
(aquatic)Tj
3.1067 0 Td
(reservoirs)Tj
-33.4769 -1.2982 Td
(during)Tj
2.9253 0 Td
(extended)Tj
3.8607 0 Td
(periods)Tj
3.1918 0 Td
(between)Tj
3.532 0 Td
(outbreaks)Tj
4.1442 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
/F9 1 Tf
-16.4578 -1.3039 Td
[()53()14()11()-9()4()]TJ
2.7212 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(O1)Tj
1.4457 0 Td
(is)Tj
0.8447 0 Td
(associated)Tj
4.2576 0 Td
(with)Tj
1.9956 0 Td
(most)Tj
2.2053 0 Td
(epidemic)Tj
3.8948 0 Td
(and)Tj
1.7291 0 Td
(pandemic)Tj
4.1783 0 Td
(outbreaks)Tj
4.1499 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(].)Tj
1.5987 0 Td
(Whole)Tj
-33.647 -1.2983 Td
(genome)Tj
3.3958 0 Td
(sequence)Tj
3.8495 0 Td
(\(WGS\))Tj
3.05 0 Td
(analysis)Tj
3.3165 0 Td
(of)Tj
/F9 1 Tf
1.0092 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.0998 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4186 0 Td
(isolates)Tj
3.1124 0 Td
(from)Tj
2.194 0 Td
(around)Tj
3.1294 0 Td
(the)Tj
1.457 0 Td
(world)Tj
2.5512 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(])Tj
2.8346 0 Td
(demon-)Tj
-34.418 -1.2983 Td
(strated)Tj
2.931 0 Td
(that)Tj
1.7801 0 Td
(the)Tj
1.4683 0 Td
(current)Tj
3.1862 0 Td
(\(Seventh\))Tj
4.0535 0 Td
(Pandemic)Tj
4.2236 0 Td
(is)Tj
0.8447 0 Td
(monophyletic)Tj
5.76 0 Td
(and)Tj
1.7347 0 Td
(originated)Tj
4.3257 0 Td
(from)Tj
2.2053 0 Td
(a)Tj
0.6577 0 Td
(single)Tj
-33.1708 -1.3039 Td
(source)Tj
2.8346 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.6519 0 Td
(clonal)Tj
2.6249 0 Td
(expansion)Tj
4.2916 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(lineage,)Tj
3.2542 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
0.652 0 Td
(strong)Tj
2.7723 0 Td
(temporal)Tj
3.8381 0 Td
(signature)Tj
3.9174 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(].)Tj
2.075 0 Td
(The)Tj
1.7858 0 Td
(sev-)Tj
-35.1777 -1.2982 Td
(enth)Tj
2.0182 0 Td
(pandemic)Tj
4.1782 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.126 0 Td
(divided)Tj
3.2144 0 Td
(into)Tj
1.8425 0 Td
(3)Tj
0.6917 0 Td
(waves)Tj
2.5795 0 Td
(beginning)Tj
4.2633 0 Td
(in)Tj
1.0318 0 Td
(1952,)Tj
2.347 0 Td
(1981)Tj
2.126 0 Td
(and)Tj
1.7291 0 Td
(1988,)Tj
2.3471 0 Td
(respectively)Tj
-32.0369 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(].)Tj
2.0749 0 Td
(Each)Tj
2.177 0 Td
(wave)Tj
2.2167 0 Td
(appears)Tj
3.2768 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(a)Tj
0.652 0 Td
(selective)Tj
3.5432 0 Td
(sweep,)Tj
2.8517 0 Td
(as)Tj
1.0148 0 Td
(was)Tj
1.7008 0 Td
(the)Tj
1.4683 0 Td
(second)Tj
3.0104 0 Td
(wave)Tj
2.2166 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1112 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(that)Tj
-33.9361 -1.2983 Td
(swept)Tj
2.5058 0 Td
(across)Tj
2.6758 0 Td
(Haiti)Tj
2.2507 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.474 0 Td
(initial)Tj
2.5455 0 Td
(introduction)Tj
5.3121 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
2.0749 0 Td
(In)Tj
1.1112 0 Td
(Africa,)Tj
2.914 0 Td
(isolates)Tj
3.1181 0 Td
(from)Tj
2.2054 0 Td
(all)Tj
1.1451 0 Td
(three)Tj
2.2677 0 Td
(waves)Tj
-33.6356 -1.3039 Td
(have)Tj
2.0579 0 Td
(been)Tj
2.126 0 Td
(identified)Tj
4.0535 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(])Tj
1.8538 0 Td
(with)Tj
1.9956 0 Td
(wave)Tj
2.2167 0 Td
(3)Tj
0.6973 0 Td
(isolates)Tj
3.1181 0 Td
(forming)Tj
3.4866 0 Td
(two)Tj
1.7064 0 Td
(distinct)Tj
3.2145 0 Td
(clades)Tj
2.6362 0 Td
(in)Tj
1.0375 0 Td
(Kenya)Tj
2.7496 0 Td
(between)Tj
-32.9497 -1.2983 Td
(2005)Tj
2.1203 0 Td
(to)Tj
1.0261 0 Td
(2010)Tj
2.1203 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
-4.0705 -1.2982 Td
(The)Tj
1.7858 0 Td
(epidemiology)Tj
5.6466 0 Td
(and)Tj
1.7291 0 Td
(transmission)Tj
5.3631 0 Td
(patterns)Tj
3.4752 0 Td
(of)Tj
1.0148 0 Td
(outbreaks)Tj
4.1443 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1111 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(have)Tj
2.058 0 Td
(been)Tj
2.1203 0 Td
(explored)Tj
-34.0892 -1.3039 Td
(using)Tj
2.3867 0 Td
(both)Tj
2.0636 0 Td
(multilocus)Tj
4.4447 0 Td
(variable)Tj
3.3562 0 Td
(number)Tj
3.4186 0 Td
(tandem)Tj
3.2711 0 Td
(repeat)Tj
2.6873 0 Td
(analysis)Tj
3.3278 0 Td
(\(MLVA\))Tj
3.7077 0 Td
(and)Tj
1.7291 0 Td
(WGS)Tj
2.3754 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
3.2542 0 Td
(In)Tj
-36.0224 -1.2983 Td
(rural)Tj
2.1713 0 Td
(Bangladesh,)Tj
4.9889 0 Td
(MLVA)Tj
3.0331 0 Td
(revealed)Tj
3.5206 0 Td
(that)Tj
1.7801 0 Td
(multiple)Tj
3.566 0 Td
(genetic)Tj
3.0727 0 Td
(lineages)Tj
3.3846 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1111 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(occur)Tj
2.4718 0 Td
(naturally)Tj
-33.5449 -1.2983 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(environment)Tj
5.4311 0 Td
(with)Tj
1.9956 0 Td
(geographic)Tj
4.6204 0 Td
(and)Tj
1.7292 0 Td
(seasonal)Tj
3.5376 0 Td
(genetic)Tj
3.067 0 Td
(variation)Tj
3.8041 0 Td
(and)Tj
1.7291 0 Td
(identical)Tj
3.6567 0 Td
(genotypes)Tj
-32.0766 -1.3039 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(found)Tj
2.6135 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(environment)Tj
5.4312 0 Td
(and)Tj
1.7348 0 Td
(humans)Tj
3.4355 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(].)Tj
2.075 0 Td
(In)Tj
1.1112 0 Td
(Kenya,)Tj
2.982 0 Td
(MLVA)Tj
3.0274 0 Td
(demonstrated)Tj
5.7599 0 Td
(that)Tj
1.7802 0 Td
(sev-)Tj
-35.2344 -1.2983 Td
(eral)Tj
1.6951 0 Td
(distinct)Tj
3.2088 0 Td
(genetic)Tj
3.0727 0 Td
(lineages)Tj
3.3902 0 Td
(emerged)Tj
3.6737 0 Td
(simultaneously)Tj
6.2418 0 Td
(during)Tj
2.9254 0 Td
(outbreaks)Tj
4.1499 0 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6576 0 Td
(single)Tj
2.5285 0 Td
(cholera)Tj
3.1521 0 Td
(sea-)Tj
-35.7276 -1.2982 Td
(son,)Tj
1.8595 0 Td
(linked)Tj
2.7325 0 Td
(to)Tj
1.0262 0 Td
(local)Tj
2.0749 0 Td
(environmental)Tj
6.1115 0 Td
(reservoirs)Tj
4.1329 0 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(].)Tj
2.0749 0 Td
(WGS)Tj
2.3754 0 Td
(revealed)Tj
3.5263 0 Td
(all)Tj
1.1452 0 Td
(lineages)Tj
3.3902 0 Td
(were)Tj
2.1203 0 Td
(part)Tj
1.8425 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-35.4271 -1.3039 Td
(Seventh)Tj
3.3618 0 Td
(Pandemic)Tj
4.2179 0 Td
(expansion)Tj
4.286 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
2.0693 0 Td
(In)Tj
1.0998 0 Td
(central)Tj
2.9594 0 Td
(and)Tj
1.7178 0 Td
(western)Tj
3.3278 0 Td
(Africa,)Tj
2.9027 0 Td
(MLVA)Tj
3.0217 0 Td
(revealed)Tj
3.5149 0 Td
(distinct)Tj
3.2088 0 Td
(clus-)Tj
-35.6879 -1.2983 Td
(ters)Tj
1.6724 0 Td
(of)Tj
1.0148 0 Td
(isolates)Tj
3.1181 0 Td
(from)Tj
2.2053 0 Td
(different)Tj
3.6567 0 Td
(countries:)Tj
4.1726 0 Td
(Democratic)Tj
4.9265 0 Td
(Republic)Tj
3.7531 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(Congo,)Tj
3.1351 0 Td
(Zambia,)Tj
3.5093 0 Td
(Togo,)Tj
-33.647 -1.2983 Td
(and)Tj
1.7291 0 Td
(Guinea)Tj
3.1351 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
2.0806 0 Td
(A)Tj
0.9014 0 Td
(second)Tj
3.0104 0 Td
(study)Tj
2.3924 0 Td
(in)Tj
1.0318 0 Td
(Guinea)Tj
3.1408 0 Td
(demonstrated)Tj
5.7599 0 Td
(spread)Tj
2.8517 0 Td
(and)Tj
1.7291 0 Td
(differentiation)Tj
5.9811 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1055 0 Td
[()13()6()]TJ
-35.8637 -1.2982 Td
[()4( )2()20(\n)6( )]TJ
/F8 1 Tf
2.0862 0 Td
(during)Tj
2.9254 0 Td
(an)Tj
1.2019 0 Td
(outbreak)Tj
3.787 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
-8.8043 -1.3039 Td
(In)Tj
1.1111 0 Td
(Mozambique,)Tj
5.743 0 Td
(previous)Tj
3.651 0 Td
(analysis)Tj
3.3278 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1055 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(O1)Tj
1.4457 0 Td
(showed)Tj
3.2485 0 Td
(these)Tj
2.2564 0 Td
(isolates)Tj
3.118 0 Td
(had)Tj
1.7065 0 Td
(the)Tj
1.4683 0 Td
(typical)Tj
-33.8227 -1.2983 Td
(traits)Tj
2.245 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(El)Tj
1.0318 0 Td
(Tor)Tj
1.7121 0 Td
(biotype)Tj
3.1918 0 Td
(overall)Tj
2.9027 0 Td
(except)Tj
2.7666 0 Td
(that)Tj
1.7745 0 Td
(they)Tj
1.9275 0 Td
(carried)Tj
3.0331 0 Td
(a)Tj
0.6519 0 Td
(tandem)Tj
3.2712 0 Td
(array)Tj
2.2847 0 Td
(of)Tj
1.0148 0 Td
(classical)Tj
3.4129 0 Td
(CTX)Tj
2.143 0 Td
(pro-)Tj
-35.8467 -1.2983 Td
(phage)Tj
2.5851 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(])Tj
1.8539 0 Td
(located)Tj
3.0784 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(small)Tj
2.3301 0 Td
(chromosome)Tj
5.4878 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(].)Tj
3.2485 0 Td
(However,)Tj
4.0819 0 Td
(there)Tj
2.262 0 Td
(are)Tj
1.4513 0 Td
(no)Tj
1.2756 0 Td
(data)Tj
1.9105 0 Td
(available)Tj
3.668 0 Td
(on)Tj
-35.977 -1.3039 Td
(the)Tj
1.4683 0 Td
(genetic)Tj
3.0671 0 Td
(relatedness)Tj
4.6431 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1055 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(circulating)Tj
4.4957 0 Td
(in)Tj
1.0375 0 Td
(Manhic)Tj
0 Tc
2.6872 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0601 0 Td
(in)Tj
1.0319 0 Td
(southern)Tj
3.787 0 Td
(Mozambique.)Tj
5.743 0 Td
(Here,)Tj
-34.5711 -1.2983 Td
(we)Tj
1.3266 0 Td
(characterized)Tj
5.5558 0 Td
(clinical)Tj
3.0784 0 Td
(and)Tj
1.7292 0 Td
(environmental)Tj
/F9 1 Tf
6.1114 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1112 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(isolates)Tj
3.1181 0 Td
(from)Tj
2.2053 0 Td
(Mozambique)Tj
5.5162 0 Td
(using)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 291.2881 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Minimal)Tj
3.6 0 Td
(genetic)Tj
3.3874 0 Td
(change)Tj
3.437 0 Td
(in)Tj
/F7 1 Tf
0.978 0 Td
(Vibrio)Tj
2.7213 0 Td
(cholerae)Tj
/F0 1 Tf
3.9827 0 Td
(in)Tj
0.978 0 Td
(Mozambique)Tj
5.8535 0 Td
(over)Tj
2.126 0 Td
(time:)Tj
2.3599 0 Td
(MLVA)Tj
2.9055 0 Td
(&)Tj
0.8788 0 Td
(WGS)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
163.5591 34.9228 m
317.7071 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Neglected)Tj
4.6417 0 Td
(Tropical)Tj
3.7135 0 Td
(Disease)Tj
3.5433 0 Td
(s)Tj
0.7158 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8197 0 Td
(1/journal.pn)Tj
5.1237 0 Td
(td.00056)Tj
3.8197 0 Td
(71)Tj
0 g
2.0905 0 Td
(June)Tj
2.3528 0 Td
(16,)Tj
1.5874 0 Td
(2017)Tj
24.9876 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(15)Tj
/F11 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(analysis,)Tj
3.4086 0 Td
(decision)Tj
3.2953 0 Td
(to)Tj
0.9497 0 Td
(publish,)Tj
3.1393 0 Td
(or)Tj
1.0064 0 Td
(prepara)Tj
2.8205 0 Td
(tion)Tj
1.6157 0 Td
(of)Tj
0.9496 0 Td
(the)Tj
-17.1851 -1.3748 Td
(manuscript.)Tj
/F10 1 Tf
0 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F11 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3749 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
45 0 obj
<>stream
endstream
endobj
46 0 obj
<>stream
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
endstream
endobj
53 0 obj
<>stream
endstream
endobj
54 0 obj
<>stream
endstream
endobj
55 0 obj
<>stream
endstream
endobj
56 0 obj
<>stream
endstream
endobj
57 0 obj
<>stream
endstream
endobj
58 0 obj
<>stream
endstream
endobj
59 0 obj
<>stream
endstream
endobj
60 0 obj
<>stream
endstream
endobj
61 0 obj
<>stream
endstream
endobj
62 0 obj
<>stream
endstream
endobj
63 0 obj
<>stream
endstream
endobj
64 0 obj
<>stream
endstream
endobj
65 0 obj
<>stream
BT
10.3394 79.2147 Td
(exist.)Tj
ET
Q
endstream
endobj
66 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 68 0 R/Contents 69 0 R/TrimBox[0 0 612 792]>>
endobj
68 0 obj
[70 0 R 71 0 R 72 0 R 73 0 R 74 0 R 75 0 R]
endobj
70 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref023)>>
endobj
71 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref024)>>
endobj
72 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref023)>>
endobj
73 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref011)>>
endobj
74 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005671.ref011)>>
endobj
75 0 obj
<>/Border[0 0 0]/A 76 0 R>>
endobj
76 0 obj
<>
endobj
69 0 obj
[77 0 R 78 0 R 79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R 85 0 R 86 0 R]
endobj
77 0 obj
<>stream
q
0.83 0.64 0.02 0 k
340.7244 606.0472 m
350.2488 606.0472 l
h
f*
352.4598 606.0472 m
361.9843 606.0472 l
h
f*
224.6173 528.0378 m
234.1417 528.0378 l
h
f*
450.7654 146.948 m
460.2331 146.948 l
h
f*
220.0252 81.9213 m
229.5496 81.9213 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(MLVA)Tj
3.0273 0 Td
(and)Tj
1.7292 0 Td
(WGS)Tj
2.381 0 Td
(to)Tj
1.0205 0 Td
(determine)Tj
4.3257 0 Td
(the)Tj
1.4683 0 Td
(genetic)Tj
3.0727 0 Td
(relatedness)Tj
4.6375 0 Td
(of)Tj
1.0148 0 Td
(strains)Tj
2.8629 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2054 0 Td
(patients)Tj
3.3675 0 Td
(with)Tj
-34.3953 -1.3039 Td
(diarrhea)Tj
3.5773 0 Td
(in)Tj
1.0318 0 Td
(Manhic)Tj
0 Tc
2.6929 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0544 0 Td
(District)Tj
3.2428 0 Td
(Hospital.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 668.1259 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Methodology)Tj
/F0 1 Tf
0 -1.5023 Td
(Site)Tj
1.9228 0 Td
(description)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 633.1464 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Manhic)Tj
0 Tc
2.6928 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0545 0 Td
(District)Tj
3.2428 0 Td
(Hospital)Tj
3.617 0 Td
(\(MDH\))Tj
3.2995 0 Td
(is)Tj
0.8448 0 Td
(a)Tj
0.6519 0 Td
(110)Tj
1.6498 0 Td
(bed)Tj
1.6781 0 Td
(referral)Tj
3.1464 0 Td
(health)Tj
2.6816 0 Td
(facility)Tj
2.897 0 Td
(for)Tj
1.3889 0 Td
(Manhic)Tj
0 Tc
2.6872 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0602 0 Td
(District,)Tj
3.4639 0 Td
(a)Tj
-36.0564 -1.3039 Td
(rural)Tj
2.1713 0 Td
(area)Tj
1.8822 0 Td
(of)Tj
1.0148 0 Td
(Maputo)Tj
3.3958 0 Td
(Province)Tj
3.7758 0 Td
(in)Tj
1.0318 0 Td
(southern)Tj
3.787 0 Td
(Mozambique.)Tj
5.743 0 Td
(The)Tj
1.7858 0 Td
(characteristics)Tj
5.9017 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(area)Tj
1.8879 0 Td
(have)Tj
-34.8602 -1.2982 Td
(been)Tj
2.1203 0 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0374 0 Td
(detail)Tj
2.4151 0 Td
(elsewhere)Tj
4.1046 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
3.2541 0 Td
(Briefly,)Tj
3.0728 0 Td
(the)Tj
1.474 0 Td
(climate)Tj
3.1294 0 Td
(is)Tj
0.8504 0 Td
(subtropical)Tj
4.6771 0 Td
(with)Tj
2.0013 0 Td
(two)Tj
1.7064 0 Td
(distinct)Tj
-33.8964 -1.2983 Td
(seasons:)Tj
3.4412 0 Td
(a)Tj
0.652 0 Td
(warm,)Tj
2.7552 0 Td
(rainy)Tj
2.2961 0 Td
(season)Tj
2.8516 0 Td
(between)Tj
3.5319 0 Td
(November)Tj
4.4617 0 Td
(and)Tj
1.7292 0 Td
(April,)Tj
2.5341 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.652 0 Td
(cool)Tj
1.9049 0 Td
(and)Tj
1.7291 0 Td
(dry)Tj
1.5761 0 Td
(season)Tj
2.8516 0 Td
(during)Tj
-34.6958 -1.3039 Td
(the)Tj
1.4683 0 Td
(rest)Tj
1.6724 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(year.)Tj
2.126 0 Td
(Manhic)Tj
0 Tc
2.6929 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0545 0 Td
(has)Tj
1.542 0 Td
(160,000)Tj
3.2995 0 Td
(inhabitants,)Tj
4.9379 0 Td
(who)Tj
1.9389 0 Td
(are)Tj
1.4457 0 Td
(mostly)Tj
2.9083 0 Td
(subsistence)Tj
4.7225 0 Td
(farmers)Tj
3.2882 0 Td
(or)Tj
-35.5859 -1.2983 Td
(workers)Tj
3.4355 0 Td
(in)Tj
1.0318 0 Td
(two)Tj
1.7121 0 Td
(large)Tj
2.16 0 Td
(sugar-)Tj
2.7269 0 Td
(and)Tj
1.7348 0 Td
(fruit-processing)Tj
6.565 0 Td
(factories.)Tj
3.8211 0 Td
(The)Tj
1.7802 0 Td
(Manhic)Tj
0 Tc
2.6928 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0545 0 Td
(Health)Tj
2.9197 0 Td
(Research)Tj
3.8041 0 Td
(Cen-)Tj
-35.4385 -1.2982 Td
(tre)Tj
1.3095 0 Td
(\(Centro)Tj
3.3733 0 Td
(de)Tj
1.1735 0 Td
(Investigac)Tj
0 Tc
3.6113 0 Td
()Tj
/F14 1 Tf
-0.004 Tc
[()]TJ
/F8 1 Tf
(o)Tj
1.5647 0 Td
(em)Tj
1.4627 0 Td
(Sau)Tj
0 Tc
0.9864 0.0396 Td
()Tj
-0.004 Tc
0.4536 -0.0396 Td
(de)Tj
1.1678 0 Td
(da)Tj
1.1849 0 Td
(Manhic)Tj
0 Tc
2.6872 0 Td
()Tj
-0.004 Tc
(a[CIS)Tj
2.6589 0 Td
(M]\))Tj
1.7915 0 Td
(is)Tj
0.8447 0 Td
(adjacent)Tj
3.5717 0 Td
(to)Tj
1.0204 0 Td
(the)Tj
1.474 0 Td
(MDH)Tj
2.6136 0 Td
(and)Tj
1.7291 0 Td
(has)Tj
-34.6788 -1.304 Td
(been)Tj
2.1203 0 Td
(conducting)Tj
4.7565 0 Td
(continuous)Tj
4.7395 0 Td
(demographic)Tj
5.5048 0 Td
(surveillance)Tj
4.9436 0 Td
(for)Tj
1.389 0 Td
(vital)Tj
1.9162 0 Td
(events)Tj
2.7326 0 Td
(and)Tj
1.7291 0 Td
(migrations)Tj
4.5581 0 Td
(since)Tj
-34.3897 -1.2982 Td
(1996)Tj
2.1203 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(],)Tj
2.0749 0 Td
(currently)Tj
3.8891 0 Td
(covering)Tj
3.6794 0 Td
(165,000)Tj
3.2995 0 Td
(individuals.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 502.8661 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Ethics)Tj
2.9149 0 Td
(statement)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 485.8582 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(strain)Tj
2.5001 0 Td
(collection)Tj
4.1102 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1112 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(study)Tj
2.3868 0 Td
(was)Tj
1.7008 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2053 0 Td
(cholera)Tj
3.1578 0 Td
(surveil-)Tj
-33.2388 -1.3039 Td
(lance)Tj
2.296 0 Td
(and)Tj
1.7291 0 Td
(other)Tj
2.3471 0 Td
(studies)Tj
2.9877 0 Td
(conducted)Tj
4.4277 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(Manhic)Tj
0 Tc
2.6929 0 Td
()Tj
-0.004 Tc
(a)Tj
1.0545 0 Td
(community)Tj
4.8869 0 Td
(by)Tj
1.1792 0 Td
(CISM)Tj
2.5908 0 Td
(approved)Tj
3.9742 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
-33.8511 -1.2982 Td
(National)Tj
3.6906 0 Td
(BioEthic)Tj
3.6567 0 Td
(Committee)Tj
4.7452 0 Td
(\(CNBS\).)Tj
3.5773 0 Td
(Any)Tj
1.9105 0 Td
(and)Tj
1.7291 0 Td
(all)Tj
1.1452 0 Td
(patient)Tj
3.0104 0 Td
(data)Tj
1.9105 0 Td
(were)Tj
2.126 0 Td
(anonymized/de-identi-)Tj
-27.5015 -1.2983 Td
(fied.)Tj
1.9502 0 Td
(The)Tj
1.7801 0 Td
(IRB)Tj
1.7632 0 Td
(at)Tj
0.9524 0 Td
(University)Tj
4.405 0 Td
(of)Tj
1.0148 0 Td
(Maryland)Tj
4.1272 0 Td
(School)Tj
2.9083 0 Td
(of)Tj
1.0148 0 Td
(Medicine)Tj
3.9912 0 Td
(approved)Tj
3.9741 0 Td
(the)Tj
1.4684 0 Td
(use)Tj
1.5364 0 Td
(of)Tj
1.0148 0 Td
(anonymized)Tj
-31.9009 -1.3039 Td
(strains.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 407.5653 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Bacterial)Tj
4.0582 0 Td
(isolates)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 390.5574 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(A)Tj
0.9014 0 Td
(total)Tj
2.0069 0 Td
(of)Tj
1.0148 0 Td
(75)Tj
/F9 1 Tf
1.1678 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1112 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(from)Tj
2.2054 0 Td
(MDH,)Tj
2.8403 0 Td
(between)Tj
3.5319 0 Td
(2002)Tj
2.1203 0 Td
(and)Tj
1.7291 0 Td
(2012.)Tj
2.3471 0 Td
(The)Tj
-33.3862 -1.2982 Td
(strains)Tj
2.8573 0 Td
(were)Tj
2.1259 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2054 0 Td
(stool)Tj
2.1429 0 Td
(of)Tj
1.0148 0 Td
(patients)Tj
3.3676 0 Td
(admitted)Tj
3.8154 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(MDH)Tj
2.6192 0 Td
(with)Tj
1.9955 0 Td
(suspicion)Tj
3.9969 0 Td
(of)Tj
1.0148 0 Td
(cholera,)Tj
-32.9327 -1.3039 Td
(presenting)Tj
4.4446 0 Td
(with)Tj
1.9956 0 Td
(watery)Tj
2.8857 0 Td
(diarrhea.)Tj
3.804 0 Td
(In)Tj
1.1056 0 Td
(addition,)Tj
3.8267 0 Td
(three)Tj
2.262 0 Td
(isolates)Tj
3.1181 0 Td
(collected)Tj
3.7417 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(Incomati)Tj
3.8551 0 Td
(River)Tj
-34.7128 -1.2983 Td
(were)Tj
2.1203 0 Td
(included.)Tj
/F9 1 Tf
3.9401 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1055 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.126 0 Td
(identified)Tj
4.0591 0 Td
(by)Tj
1.1792 0 Td
(standard)Tj
3.7247 0 Td
(biochemical)Tj
5.0627 0 Td
(tests;)Tj
2.1883 0 Td
(and)Tj
1.7291 0 Td
(con-)Tj
-33.783 -1.2983 Td
(firmed)Tj
2.9139 0 Td
(by)Tj
1.1849 0 Td
(API-20E)Tj
3.6737 0 Td
(biochemical)Tj
5.0683 0 Td
(test)Tj
1.5987 0 Td
(strips)Tj
2.3981 0 Td
(\(bioMe)Tj
0 Tc
2.5342 0.0511 Td
()Tj
-0.004 Tc
0.4025 -0.0511 Td
(rieux)Tj
2.2734 0 Td
(SA,)Tj
1.6044 0 Td
(Marcy-l'Etoile,)Tj
6.1455 0 Td
(France\).)Tj
3.5149 0 Td
(Serotypes)Tj
-33.3125 -1.2982 Td
(were)Tj
2.1203 0 Td
(determined)Tj
4.8528 0 Td
(using)Tj
2.3868 0 Td
(commercially)Tj
5.6579 0 Td
(available)Tj
3.668 0 Td
(poly-)Tj
2.2961 0 Td
(and)Tj
1.7291 0 Td
(mono-clonal)Tj
5.3574 0 Td
(slide)Tj
2.0466 0 Td
(agglutination)Tj
5.5276 0 Td
(anti-)Tj
-35.6426 -1.3039 Td
(sera)Tj
1.8084 0 Td
(\(Mast)Tj
2.5455 0 Td
(Group)Tj
2.8573 0 Td
(Ltd.,)Tj
2.0353 0 Td
(Merseyside,)Tj
4.9549 0 Td
(UK\))Tj
1.9673 0 Td
(according)Tj
4.1895 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(manufacturer's)Tj
6.2645 0 Td
(instructions.)Tj
5.2214 0 Td
(All)Tj
1.4003 0 Td
(the)Tj
-35.7389 -1.2983 Td
(isolates)Tj
3.118 0 Td
(were)Tj
2.1203 0 Td
(stored)Tj
2.7099 0 Td
(at)Tj
0.9525 0 Td
(-80C)Tj
2.5795 0 Td
(in)Tj
1.0318 0 Td
(tryptone)Tj
3.6397 0 Td
(soya)Tj
1.9785 0 Td
(broth)Tj
2.4321 0 Td
(\(TSB\))Tj
2.5625 0 Td
(with)Tj
2.0013 0 Td
(15%)Tj
1.9275 0 Td
(glycerol,)Tj
3.583 0 Td
(and)Tj
1.7291 0 Td
(retrieved)Tj
3.7814 0 Td
(at)Tj
-36.1471 -1.2983 Td
(the)Tj
1.4683 0 Td
(time)Tj
2.0296 0 Td
(for)Tj
1.3833 0 Td
(molecular)Tj
4.2236 0 Td
(characterization.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 260.2771 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Preparation)Tj
5.3244 0 Td
(of)Tj
1.0441 0 Td
(bacterial)Tj
3.9496 0 Td
(DNA)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 243.2692 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(A)Tj
0.9014 0 Td
(pure)Tj
2.0636 0 Td
(culture)Tj
3.0387 0 Td
(of)Tj
/F9 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F9 1 Tf
1.1055 0 Td
[()13()6()10()4( )2()20(\n)6( )]TJ
/F8 1 Tf
3.4299 0 Td
(was)Tj
1.7008 0 Td
(plated)Tj
2.6645 0 Td
(in)Tj
1.0375 0 Td
(Thiosulfate)Tj
4.7111 0 Td
(Citrate)Tj
2.9764 0 Td
(Bile)Tj
1.7348 0 Td
(Sucrose)Tj
3.3108 0 Td
(\(TCBS\))Tj
3.2315 0 Td
(agar)Tj
1.9219 0 Td
(and)Tj
-34.8432 -1.2982 Td
(incubated)Tj
4.1782 0 Td
(overnight)Tj
4.0819 0 Td
(at)Tj
0.9524 0 Td
(37C.)Tj
2.4491 0 Td
(DNA)Tj
2.3811 0 Td
(was)Tj
1.6951 0 Td
(extracted)Tj
3.8891 0 Td
(using)Tj
2.3868 0 Td
(the)Tj
1.4683 0 Td
(Qiagen)Tj
3.1068 0 Td
(QIAamp)Tj
3.7643 0 Td
(DNA)Tj
2.3755 0 Td
(Mini)Tj
2.194 0 Td
(Kit)Tj
-34.9226 -1.2983 Td
(\(Hilden,)Tj
3.5659 0 Td
(Germany\).)Tj
4.5524 0 Td
(The)Tj
1.7802 0 Td
(DNA)Tj
2.3754 0 Td
(template)Tj
3.685 0 Td
(was)Tj
1.6951 0 Td
(sent)Tj
1.8539 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(University)Tj
4.405 0 Td
(of)Tj
1.0148 0 Td
(Maryland)Tj
4.1273 0 Td
(Baltimore,)Tj
4.3936 0 Td
(Bal-)Tj
-35.943 -1.3039 Td
(timore,)Tj
3.1294 0 Td
(Maryland,)Tj
4.354 0 Td
(USA)Tj
2.1203 0 Td
(for)Tj
1.3833 0 Td
(molecular)Tj
4.2179 0 Td
(typing)Tj
2.7723 0 Td
(by)Tj
1.1848 0 Td
(MLVA)Tj
3.0274 0 Td
(and)Tj
1.7291 0 Td
(WGS.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 178.0157 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(MLVA)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 161.0078 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(DNA)Tj
2.3754 0 Td
(from)Tj
2.2053 0 Td
(each)Tj
2.0296 0 Td
(isolate)Tj
2.7553 0 Td
(was)Tj
1.7007 0 Td
(amplified)Tj
4.0082 0 Td
(by)Tj
1.1849 0 Td
(PCR)Tj
2.0636 0 Td
(using)Tj
2.3867 0 Td
(the)Tj
1.474 0 Td
(conditions)Tj
4.4731 0 Td
(and)Tj
1.7291 0 Td
(primers)Tj
3.3505 0 Td
(previously)Tj
-31.7364 -1.2982 Td
(described)Tj
4.0535 0 Td
(for)Tj
1.3833 0 Td
(5)Tj
0.6973 0 Td
(loci)Tj
1.6611 0 Td
(containing)Tj
4.5297 0 Td
(variable)Tj
3.3562 0 Td
(length)Tj
2.7326 0 Td
(tandem)Tj
3.2712 0 Td
(repeats)Tj
3.05 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
2.075 0 Td
(The)Tj
1.7801 0 Td
(amplified)Tj
4.0138 0 Td
(products)Tj
-32.6038 -1.3039 Td
(were)Tj
2.1203 0 Td
(separated)Tj
4.0138 0 Td
(and)Tj
1.7291 0 Td
(detected)Tj
3.566 0 Td
(using)Tj
2.3867 0 Td
(a)Tj
0.6577 0 Td
(model)Tj
2.7439 0 Td
(3730xl)Tj
2.8346 0 Td
(Automatic)Tj
4.4787 0 Td
(Sequencer)Tj
4.3427 0 Td
(\(ABI\))Tj
2.5058 0 Td
(and)Tj
1.7291 0 Td
(their)Tj
2.109 0 Td
(sizes)Tj
-35.2174 -1.2983 Td
(were)Tj
2.1203 0 Td
(determined)Tj
4.8528 0 Td
(using)Tj
2.3868 0 Td
(internal)Tj
3.3619 0 Td
(lane)Tj
1.8708 0 Td
(standards)Tj
4.0819 0 Td
(\(Liz600;)Tj
3.4299 0 Td
(ABI,)Tj
2.0522 0 Td
(Foster)Tj
2.71 0 Td
(City,)Tj
2.1259 0 Td
(CA\))Tj
1.9106 0 Td
(with)Tj
1.9955 0 Td
(the)Tj
1.4684 0 Td
(Gene)Tj
-34.367 -1.2983 Td
(Mapper)Tj
3.3732 0 Td
(v4.0)Tj
1.8538 0 Td
(program)Tj
3.7077 0 Td
(\(ABI\).)Tj
2.7269 0 Td
(Genotypes)Tj
4.4674 0 Td
(were)Tj
2.126 0 Td
(determined)Tj
4.8472 0 Td
(according)Tj
4.1895 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(published)Tj
4.1272 0 Td
(formulas)Tj
-33.9134 -1.3039 Td
(to)Tj
1.0261 0 Td
(calculate)Tj
3.6737 0 Td
(the)Tj
1.4683 0 Td
(number)Tj
3.4186 0 Td
(of)Tj
1.0148 0 Td
(repeats)Tj
3.05 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(length)Tj
2.7326 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(allele)Tj
2.2393 0 Td
(and)Tj
1.7291 0 Td
(identify)Tj
3.2939 0 Td
(the)Tj
1.4683 0 Td
(alleles)Tj
2.6022 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4684 0 Td
(5)Tj
-36.8558 -1.2982 Td
(loci)Tj
1.661 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
2.0807 0 Td
(The)Tj
1.7801 0 Td
(5)Tj
0.6973 0 Td
(loci,)Tj
1.8879 0 Td
(in)Tj
1.0375 0 Td
(order,)Tj
2.6418 0 Td
(are)Tj
1.4514 0 Td
(VC0147,)Tj
3.7077 0 Td
(VC04367)Tj
4.473 0 Td
(\(intergenic\),)Tj
5.159 0 Td
(VC1650,)Tj
3.7077 0 Td
(VCA0171,)Tj
4.388 0 Td
(and)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 291.2881 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Minimal)Tj
3.6 0 Td
(genetic)Tj
3.3874 0 Td
(change)Tj
3.437 0 Td
(in)Tj
/F7 1 Tf
0.978 0 Td
(Vibrio)Tj
2.7213 0 Td
(cholerae)Tj
/F0 1 Tf
3.9827 0 Td
(in)Tj
0.978 0 Td
(Mozambique)Tj
5.8535 0 Td
(over)Tj
2.126 0 Td
(time:)Tj
2.3599 0 Td
(MLVA)Tj
2.9055 0 Td
(&)Tj
0.8788 0 Td
(WGS)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
163.5591 34.9228 m
317.7071 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Neglected)Tj
4.6417 0 Td
(Tropical)Tj
3.7135 0 Td
(Disease)Tj
3.5433 0 Td
(s)Tj
0.7158 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8197 0 Td
(1/journal.pn)Tj
5.1237 0 Td
(td.00056)Tj
3.8197 0 Td
(71)Tj
0 g
2.0905 0 Td
(June)Tj
2.3528 0 Td
(16,)Tj
1.5874 0 Td
(2017)Tj
24.9876 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
78 0 obj
<>stream
endstream
endobj
79 0 obj
<>stream
endstream
endobj
80 0 obj
<>stream
endstream
endobj
81 0 obj
<>stream
endstream
endobj
82 0 obj
<>stream
endstream
endobj
83 0 obj
<>stream
endstream
endobj
84 0 obj
<>stream
endstream
endobj
85 0 obj
<>stream
endstream
endobj
86 0 obj
<>stream
BT
66.402 0 Td
(15)Tj
ET
Q
endstream
endobj
87 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 89 0 R/Contents 90 0 R/TrimBox[0 0 612 792]>>
endobj
88 0 obj
<>
endobj
91 0 obj
<>
endobj
92 0 obj
<>stream
%!PS-AdobeFont-1.0
11 dict begin
/FontName /TeX_CM_Maths_Symbols def
/FontType 1 def
/FontBBox {-20 -958 1114 770} readonly def
/FontMatrix [0.001 0 0 0.001 0 0] readonly def
/PaintType 0 def
/FontInfo 8 dict dup begin
/FullName (TeX_CM_Maths_Symbols) readonly def
/BaseFontName (TeX_CM_Maths_Symbols) def
end def
/Encoding 256 array
0 1 255 {1 index exch /.notdef put} for
dup 0 /C0 put
dup 1 /C1 put
dup 2 /C2 put
dup 3 /C3 put
dup 4 /C4 put
dup 5 /C5 put
dup 6 /C6 put
dup 7 /C7 put
dup 8 /C8 put
dup 9 /C9 put
dup 10 /C10 put
dup 11 /C11 put
dup 12 /C12 put
dup 13 /C13 put
dup 14 /C14 put
dup 15 /C15 put
dup 16 /C16 put
dup 17 /C17 put
dup 18 /C18 put
dup 19 /C19 put
dup 20 /C20 put
dup 21 /C21 put
dup 22 /C22 put
dup 23 /C23 put
dup 24 /C24 put
dup 25 /C25 put
dup 26 /C26 put
dup 27 /C27 put
dup 28 /C28 put
dup 29 /C29 put
dup 30 /C30 put
dup 31 /C31 put
dup 32 /space put
dup 33 /exclam put
dup 34 /quotedbl put
dup 35 /numbersign put
dup 36 /dollar put
dup 37 /percent put
dup 38 /ampersand put
dup 39 /quoteright put
dup 40 /parenleft put
dup 41 /parenright put
dup 42 /asterisk put
dup 43 /plus put
dup 44 /comma put
dup 45 /hyphen put
dup 46 /period put
dup 47 /slash put
dup 48 /zero put
dup 49 /one put
dup 50 /two put
dup 51 /three put
dup 52 /four put
dup 53 /five put
dup 54 /six put
dup 55 /seven put
dup 56 /eight put
dup 57 /nine put
dup 58 /colon put
dup 59 /semicolon put
dup 60 /less put
dup 61 /equal put
dup 62 /greater put
dup 63 /question put
dup 64 /at put
dup 65 /A put
dup 66 /B put
dup 67 /C put
dup 68 /D put
dup 69 /E put
dup 70 /F put
dup 71 /G put
dup 72 /H put
dup 73 /I put
dup 74 /J put
dup 75 /K put
dup 76 /L put
dup 77 /M put
dup 78 /N put
dup 79 /O put
dup 80 /P put
dup 81 /Q put
dup 82 /R put
dup 83 /S put
dup 84 /T put
dup 85 /U put
dup 86 /V put
dup 87 /W put
dup 88 /X put
dup 89 /Y put
dup 90 /Z put
dup 91 /bracketleft put
dup 92 /backslash put
dup 93 /bracketright put
dup 94 /asciicircum put
dup 95 /underscore put
dup 96 /quoteleft put
dup 97 /a put
dup 98 /b put
dup 99 /c put
dup 100 /d put
dup 101 /e put
dup 102 /f put
dup 103 /g put
dup 104 /h put
dup 105 /i put
dup 106 /j put
dup 107 /k put
dup 108 /l put
dup 109 /m put
dup 110 /n put
dup 111 /o put
dup 112 /p put
dup 113 /q put
dup 114 /r put
dup 115 /s put
dup 116 /t put
dup 117 /u put
dup 118 /v put
dup 119 /w put
dup 120 /x put
dup 121 /y put
dup 122 /z put
dup 123 /braceleft put
dup 124 /bar put
dup 125 /braceright put
dup 126 /asciitilde put
dup 127 /Euro put
dup 128 /ff put
dup 129 /ffi put
dup 130 /ffl put
dup 131 /C131 put
dup 132 /Eth put
dup 133 /eth put
dup 134 /Thorn put
dup 135 /thorn put
dup 136 /onequarter put
dup 137 /onehalf put
dup 138 /C138 put
readonly def
currentdict end
currentfile eexec
M#1H5 J6o\&rtS(#v\0\V7Fe༙OYx5-cY9^%Y6
(efIOldN"wȰ;)UƽoFg/rvV$JS8_oc@
o9-eGJp}[Do]mN=4*{ܯq=eU^t6¤(HwK7X7
Mr:E%]3<Ӝ$wrAQ4ADt%̏>E hAجm*pu@>#6aZi}NRmƼ%
,OgX' EX?ڞ\#JEG<IXS}Eh[ş8ї48ٶ.w4bJ?_nfulqD?둓\C%+' { L9K7isW+ӕ(6oɧVҺlS;5K
Z%.oQՕW*9Qk_S+B-}%lYC@WP0b\KKdi>t,n_S[ '9`-z+cڦEb#c@;yɸoAT O_H~i -6ϰ豟Ae&BF+
wNX!=hhUF8(hZ6pgJc m'9dx*^wXc
գ<.쯶c ln)/LvXY2jbF:ȸ3'>\Ȥmzj,!:ZK' (q/ -&&S ؤ-bm KQSxLGu `D3yԱKaVMU§ %iq5%s 2SdR#ho45H.4TdՊa<K>BqG- Ao, (e&F2gG`z8*༠&
x#B
B;8!93~T/ \>nLazCSKm~,!_<ӾX@X@N2vEt בFmBYa wRG[i;+oM+%(8 jԀlIW>t