%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Thais C. de Oliveira, Priscila T. Rodrigues, Maria José Menezes, Raquel M. Gonçalves-Lopes, Melissa S. Bastos, Nathália F. Lima, Susana Barbosa, Alexandra L. Gerber, Guilherme Loss de Morais, Luisa Berná, Jody Phelan, Carlos Robello, Ana Tereza R. de Vasconcelos, João Marcelo P. Alves, Marcelo U. Ferreira
Genome-wide diversity and differentiation in New World populations of the human malaria parasite Plasmodium vivax
www.plosntds.org
www.plosntds.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 14 0 R/Annots 15 0 R/Contents 16 0 R/TrimBox[0 0 612 792]>>
endobj
15 0 obj
[17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R]
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref001)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref002)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref003)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref004)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref001)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref005)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref007)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref008)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref008)>>
endobj
26 0 obj
<>/Border[0 0 0]/A 30 0 R>>
endobj
30 0 obj
<>
endobj
27 0 obj
<>/Border[0 0 0]/A 31 0 R>>
endobj
31 0 obj
<>
endobj
28 0 obj
<>/Border[0 0 0]/A 32 0 R>>
endobj
32 0 obj
<>
endobj
29 0 obj
<>/Border[0 0 0]/A 33 0 R>>
endobj
33 0 obj
<>
endobj
16 0 obj
[34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R]
endobj
34 0 obj
<>stream
q
0.955 g
0 w
199.9998 571.2299 m
575.9997 571.2299 l
575.9997 345.2304 l
199.9998 345.2304 l
f*
0.45 0.36 0.35 0.01 k
575.9997 596.2297 m
199.9998 596.2297 l
199.9998 596.7298 l
575.9997 596.7298 l
f*
0.83 0.64 0.02 0 k
513.5811 250.9228 m
518.3433 250.9228 l
h
f*
246.0472 224.9008 m
250.7528 224.9008 l
h
f*
253.0205 224.9008 m
257.7827 224.9008 l
h
f*
339.0803 198.9354 m
343.7858 198.9354 l
h
f*
536.3717 172.9134 m
541.1339 172.9134 l
h
f*
518.8535 146.948 m
523.6157 146.948 l
h
f*
528.8315 146.948 m
533.537 146.948 l
h
f*
203.4142 94.9039 m
208.1764 94.9039 l
h
f*
221.1024 81.9213 m
225.8646 81.9213 l
h
f*
0 g
1 j
1 J
9.5 0 0 9.5 200.0125 707.7543 cm
BT
/F0 1 Tf
1 TL
-0.0042 Tc
0 0 Td
(populations,)Tj
5.5439 0 Td
(with)Tj
1.9753 0 Td
(relatively)Tj
4.1117 0 Td
(little)Tj
1.9634 0 Td
(between-po)Tj
5.168 0 Td
(pulation)Tj
3.6701 0 Td
(genome-wide)Tj
6.1526 0 Td
(differentiation)Tj
6.1407 0 Td
(\(pairwise)Tj
/F9 1 Tf
-34.7257 -1.474 Td
(F)Tj
ET
Q
q
1 j
1 J
0 w
7.1248 0 0 6.3325 205.7952 692.1637 cm
BT
/F0 1 Tf
1 TL
-0.0056 Tc
0 0 Td
(ST)Tj
ET
Q
q
1 j
1 J
0 w
9.5 0 0 9.5 216.9637 693.7511 cm
BT
/F0 1 Tf
1 TL
-0.0042 Tc
0 0 Td
(values)Tj
3.0733 0 Td
(ranging)Tj
3.509 0 Td
(between)Tj
3.9565 0 Td
(0.025)Tj
2.6914 0 Td
(and)Tj
1.8679 0 Td
(0.092\).)Tj
3.2942 0 Td
(Finally,)Tj
/F9 1 Tf
3.3478 0 Td
(P)Tj
/F0 1 Tf
(.)Tj
/F9 1 Tf
1.1637 0 Td
(vivax)Tj
/F0 1 Tf
2.4706 0 Td
(populations)Tj
5.2695 0 Td
(show)Tj
2.5243 0 Td
(a)Tj
0.7698 0 Td
(rapid)Tj
-35.7223 -1.474 Td
(decline)Tj
3.3418 0 Td
(in)Tj
0.9787 0 Td
(linkage)Tj
3.3419 0 Td
(disequilibriu)Tj
5.1978 0 Td
(m)Tj
1.0503 0 Td
(with)Tj
1.9753 0 Td
(increasing)Tj
4.7145 0 Td
(distance)Tj
3.9028 0 Td
(between)Tj
3.9566 0 Td
(pairs)Tj
2.3572 0 Td
(of)Tj
1.0443 0 Td
(polymorphic)Tj
-31.8612 -1.474 Td
(sites,)Tj
2.5302 0 Td
(consistent)Tj
4.6727 0 Td
(with)Tj
1.9812 0 Td
(very)Tj
2.0887 0 Td
(frequent)Tj
3.8492 0 Td
(outcrossing.)Tj
5.5499 0 Td
(We)Tj
1.7187 0 Td
(hypothesize)Tj
5.4902 0 Td
(that)Tj
1.8739 0 Td
(the)Tj
1.5933 0 Td
(high)Tj
2.0827 0 Td
(diversity)Tj
3.8432 0 Td
(of)Tj
-37.2739 -1.474 Td
(present-day)Tj
/F9 1 Tf
5.4424 0 Td
(P)Tj
/F0 1 Tf
(.)Tj
/F9 1 Tf
1.1577 0 Td
(vivax)Tj
/F0 1 Tf
2.4766 0 Td
(lineages)Tj
3.8909 0 Td
(in)Tj
0.9787 0 Td
(the)Tj
1.5994 0 Td
(Americas)Tj
4.3444 0 Td
(originated)Tj
4.5533 0 Td
(from)Tj
2.2021 0 Td
(successive)Tj
5.0606 0 Td
(migratory)Tj
4.3444 0 Td
(waves)Tj
-36.0505 -1.474 Td
(and)Tj
1.8618 0 Td
(subsequen)Tj
4.8398 0 Td
(t)Tj
0.4953 0 Td
(admixture)Tj
4.5653 0 Td
(between)Tj
3.9565 0 Td
(parasite)Tj
3.7298 0 Td
(lineages)Tj
3.8909 0 Td
(from)Tj
2.2081 0 Td
(geographically)Tj
6.5763 0 Td
(diverse)Tj
3.4016 0 Td
(sites.)Tj
-35.5254 -1.474 Td
(Further)Tj
3.4134 0 Td
(genome-wide)Tj
6.1527 0 Td
(analyses)Tj
4.1177 0 Td
(are)Tj
1.647 0 Td
(required)Tj
3.8372 0 Td
(to)Tj
1.0444 0 Td
(test)Tj
1.8201 0 Td
(the)Tj
1.5993 0 Td
(demographic)Tj
5.9319 0 Td
(scenario)Tj
3.9506 0 Td
(suggested)Tj
4.786 0 Td
(by)Tj
-38.3003 -1.474 Td
(our)Tj
1.6471 0 Td
(data.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 209.9905 553.2094 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Author)Tj
3.137 0 Td
(summary)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 209.9905 535.2377 cm
BT
/F15 1 Tf
1 TL
-0.004 Tc
0 0 Td
[()4()4()10()6()4()12()12( )12()]TJ
5.1363 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3017 0 Td
(is)Tj
0.8504 0 Td
(the)Tj
1.4683 0 Td
(most)Tj
2.2054 0 Td
(common)Tj
3.8437 0 Td
(human)Tj
3.0784 0 Td
(malaria)Tj
3.2258 0 Td
(parasite)Tj
3.3279 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(Americas,)Tj
4.2293 0 Td
(but)Tj
1.5534 0 Td
(how)Tj
-33.7264 -1.2982 Td
(and)Tj
1.7291 0 Td
(when)Tj
2.4037 0 Td
(this)Tj
1.6781 0 Td
(species)Tj
2.9934 0 Td
(arrived)Tj
3.0727 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4741 0 Td
(New)Tj
2.0636 0 Td
(World)Tj
2.8346 0 Td
(remains)Tj
3.4469 0 Td
(unclear.)Tj
3.4186 0 Td
(Here)Tj
2.2053 0 Td
(we)Tj
1.3323 0 Td
(describe)Tj
3.5206 0 Td
(high-)Tj
-33.2048 -1.3039 Td
(quality)Tj
2.9536 0 Td
(whole-genome)Tj
6.1512 0 Td
(sequence)Tj
3.8551 0 Td
(data)Tj
1.9162 0 Td
(for)Tj
1.3833 0 Td
(nine)Tj
/F15 1 Tf
2.0069 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9978 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3017 0 Td
(isolates)Tj
3.1181 0 Td
(from)Tj
2.211 0 Td
(Brazil,)Tj
2.7553 0 Td
(a)Tj
0.6519 0 Td
(country)Tj
3.3506 0 Td
(that)Tj
-33.6527 -1.2983 Td
(accounts)Tj
3.7417 0 Td
(for)Tj
1.3889 0 Td
(37%)Tj
1.9276 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(malaria)Tj
3.2258 0 Td
(burden)Tj
3.1294 0 Td
(in)Tj
1.0318 0 Td
(this)Tj
1.6781 0 Td
(continent,)Tj
4.3087 0 Td
(and)Tj
1.7291 0 Td
(compare)Tj
3.719 0 Td
(these)Tj
2.2507 0 Td
(data)Tj
1.9162 0 Td
(with)Tj
-32.5301 -1.2983 Td
(additional)Tj
4.2802 0 Td
(publicly)Tj
3.4073 0 Td
(available)Tj
/F15 1 Tf
3.668 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(genomes)Tj
3.77 0 Td
(from)Tj
2.2054 0 Td
(Brazil,)Tj
2.7552 0 Td
(Peru,)Tj
2.3301 0 Td
(Colombia,)Tj
4.3937 0 Td
(and)Tj
1.7291 0 Td
(Mexico.)Tj
/F15 1 Tf
-31.8385 -1.3039 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(populations)Tj
4.9549 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(New)Tj
2.0636 0 Td
(World)Tj
2.8403 0 Td
(were)Tj
2.1203 0 Td
(found)Tj
2.6192 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(as)Tj
1.0148 0 Td
(diverse)Tj
3.0443 0 Td
(as)Tj
1.0205 0 Td
(their)Tj
2.1033 0 Td
(counter-)Tj
-30.9314 -1.2982 Td
(parts)Tj
2.2053 0 Td
(from)Tj
2.2053 0 Td
(areas)Tj
2.2394 0 Td
(with)Tj
2.0012 0 Td
(substantially)Tj
5.2044 0 Td
(higher)Tj
2.8006 0 Td
(malaria)Tj
3.2258 0 Td
(transmission,)Tj
5.5899 0 Td
(such)Tj
2.058 0 Td
(as)Tj
1.0148 0 Td
(Southeast)Tj
4.0761 0 Td
(Asia,)Tj
-32.6208 -1.2983 Td
(and)Tj
1.7291 0 Td
(to)Tj
1.0261 0 Td
(carry)Tj
2.2734 0 Td
(several)Tj
2.9367 0 Td
(non-synonymous)Tj
7.2623 0 Td
(substitutions)Tj
5.3517 0 Td
(in)Tj
1.0375 0 Td
(candidate)Tj
4.1046 0 Td
(drug-resistance)Tj
6.3722 0 Td
(genes.)Tj
-32.0936 -1.3039 Td
(Moreover,)Tj
4.3993 0 Td
(genome-wide)Tj
5.6579 0 Td
(patterns)Tj
3.4753 0 Td
(of)Tj
1.0148 0 Td
(linkage)Tj
3.101 0 Td
(disequilibrium)Tj
6.1058 0 Td
(between)Tj
3.5263 0 Td
(pairs)Tj
2.1713 0 Td
(of)Tj
1.0148 0 Td
(polymorphic)Tj
-30.4665 -1.2983 Td
(sites)Tj
1.9332 0 Td
(are)Tj
1.4456 0 Td
(consistent)Tj
4.2576 0 Td
(with)Tj
1.9956 0 Td
(very)Tj
1.9276 0 Td
(frequent)Tj
3.6056 0 Td
(outcrossing)Tj
4.8586 0 Td
(in)Tj
1.0374 0 Td
(these)Tj
2.2507 0 Td
(populations.)Tj
5.1817 0 Td
(Interestingly,)Tj
-28.4936 -1.2983 Td
(local)Tj
/F15 1 Tf
2.0749 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9978 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(is)Tj
0.8447 0 Td
(more)Tj
2.3414 0 Td
(polymorphic,)Tj
5.6069 0 Td
(with)Tj
1.9956 0 Td
(less)Tj
1.61 0 Td
(between-population)Tj
8.2488 0 Td
(differentiation,)Tj
6.2078 0 Td
(than)Tj
-32.2353 -1.2982 Td
(sympatric)Tj
4.1612 0 Td
(populations)Tj
4.9436 0 Td
(of)Tj
/F15 1 Tf
1.0091 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9865 0 Td
[()9()11()4(\r)1()10()4()16()-9( )12()]TJ
/F10 1 Tf
(,)Tj
4.6941 0 Td
(possibly)Tj
3.4356 0 Td
(as)Tj
1.0091 0 Td
(a)Tj
0.6463 0 Td
(result)Tj
2.4434 0 Td
(of)Tj
1.0092 0 Td
(different)Tj
3.651 0 Td
(demographic)Tj
5.4935 0 Td
(histo-)Tj
-33.4826 -1.3039 Td
(ries)Tj
1.644 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2564 0 Td
(two)Tj
1.7064 0 Td
(species)Tj
2.9991 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(Americas.)Tj
4.2293 0 Td
(We)Tj
1.6044 0 Td
(hypothesize)Tj
4.9435 0 Td
(that)Tj
1.7802 0 Td
(local)Tj
/F15 1 Tf
2.0749 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9922 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3073 0 Td
(lineages)Tj
3.3903 0 Td
(origi-)Tj
-33.4486 -1.2983 Td
(nated)Tj
2.4547 0 Td
(from)Tj
2.2054 0 Td
(successive)Tj
4.2463 0 Td
(migratory)Tj
4.2065 0 Td
(waves)Tj
2.5795 0 Td
(and)Tj
1.7292 0 Td
(subsequent)Tj
4.7055 0 Td
(admixture)Tj
4.3539 0 Td
(between)Tj
3.5263 0 Td
(parasites)Tj
3.6907 0 Td
(from)Tj
-33.698 -1.2983 Td
(geographically)Tj
6.0037 0 Td
(diverse)Tj
3.0501 0 Td
(sites.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 308.0125 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 291.0047 cm
BT
/F15 1 Tf
1 TL
-0.004 Tc
0 0 Td
[()4()4()10()6()4()12()12( )12()]TJ
5.1306 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(is)Tj
0.8447 0 Td
(the)Tj
1.474 0 Td
(human)Tj
3.0784 0 Td
(malaria)Tj
3.2259 0 Td
(parasite)Tj
3.3221 0 Td
(with)Tj
2.0013 0 Td
(the)Tj
1.4683 0 Td
(widest)Tj
2.7836 0 Td
(global)Tj
2.6249 0 Td
(distribution)Tj
4.9663 0 Td
(and)Tj
-33.2275 -1.2982 Td
(accounts)Tj
3.736 0 Td
(for)Tj
1.3776 0 Td
(nearly)Tj
2.6872 0 Td
(half)Tj
1.7065 0 Td
(of)Tj
1.0091 0 Td
(the)Tj
1.4627 0 Td
(combined)Tj
4.2349 0 Td
(malaria)Tj
3.2145 0 Td
(burden)Tj
3.1181 0 Td
(in)Tj
1.0261 0 Td
(South)Tj
2.5512 0 Td
(and)Tj
1.7178 0 Td
(Southeast)Tj
4.0648 0 Td
(Asia,)Tj
2.1827 0 Td
(Oceania,)Tj
-34.0892 -1.3039 Td
(and)Tj
1.7291 0 Td
(Central)Tj
3.2031 0 Td
(and)Tj
1.7348 0 Td
(South)Tj
2.5512 0 Td
(America.)Tj
3.8721 0 Td
(Over)Tj
2.2166 0 Td
(one-third)Tj
4.0422 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(world's)Tj
3.1294 0 Td
(population)Tj
4.5921 0 Td
(is)Tj
0.8504 0 Td
(currently)Tj
3.8834 0 Td
(at)Tj
0.9524 0 Td
(risk)Tj
-35.24 -1.2983 Td
(of)Tj
1.0147 0 Td
(infection)Tj
3.7928 0 Td
(with)Tj
1.9956 0 Td
(this)Tj
1.6724 0 Td
(species,)Tj
3.2258 0 Td
(with)Tj
1.9956 0 Td
(16)Tj
1.1678 0 Td
(million)Tj
3.1238 0 Td
(clinical)Tj
3.0784 0 Td
(cases)Tj
2.2224 0 Td
(recorded)Tj
3.7927 0 Td
(each)Tj
2.0296 0 Td
(year)Tj
1.9048 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(].)Tj
1.5988 0 Td
(Although)Tj
/F15 1 Tf
4.0081 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
-36.6233 -1.2983 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3073 0 Td
(has)Tj
1.5421 0 Td
(most)Tj
2.2053 0 Td
(likely)Tj
2.3528 0 Td
(evolved)Tj
3.2598 0 Td
(from)Tj
2.2053 0 Td
(parasites)Tj
3.685 0 Td
(that)Tj
1.7802 0 Td
(infect)Tj
2.4661 0 Td
(chimpanzees)Tj
5.3801 0 Td
(and)Tj
1.7292 0 Td
(gorillas)Tj
3.118 0 Td
(in)Tj
1.0375 0 Td
(sub-Saha-)Tj
-33.0687 -1.3039 Td
(ran)Tj
1.5703 0 Td
(Africa)Tj
2.6929 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(],)Tj
2.3017 0 Td
(it)Tj
0.7824 0 Td
(is)Tj
0.8504 0 Td
(nowadays)Tj
4.1669 0 Td
(rare)Tj
1.8198 0 Td
(in)Tj
1.0375 0 Td
(most)Tj
2.2053 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6781 0 Td
(continent,)Tj
4.3087 0 Td
(where)Tj
2.6475 0 Td
(human)Tj
3.0784 0 Td
(populations)Tj
4.9493 0 Td
(lack)Tj
1.8198 0 Td
(a)Tj
-36.9238 -1.2982 Td
(key)Tj
1.593 0 Td
(erythrocyte)Tj
4.7735 0 Td
(receptor)Tj
3.5546 0 Td
(for)Tj
1.389 0 Td
(host)Tj
1.9106 0 Td
(cell)Tj
1.5647 0 Td
(invasion)Tj
3.6056 0 Td
(by)Tj
1.1792 0 Td
(blood-stage)Tj
4.8472 0 Td
(parasites,)Tj
3.9175 0 Td
(the)Tj
1.4683 0 Td
(Duffy)Tj
2.5115 0 Td
(antigen/)Tj
-32.3147 -1.2983 Td
(receptor)Tj
3.5546 0 Td
(for)Tj
1.3833 0 Td
(chemokines)Tj
5.0229 0 Td
(\(DARC\))Tj
3.6057 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(].)Tj
1.5987 0 Td
(Where)Tj
2.931 0 Td
(both)Tj
2.058 0 Td
(species)Tj
2.999 0 Td
(coexist,)Tj
/F15 1 Tf
3.1918 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(typically)Tj
3.5603 0 Td
(causes)Tj
2.7552 0 Td
(less)Tj
-35.96 -1.3039 Td
(severe)Tj
2.6758 0 Td
(cases)Tj
2.2224 0 Td
(and)Tj
1.7291 0 Td
(fewer)Tj
2.4151 0 Td
(deaths)Tj
2.7893 0 Td
(than)Tj
/F15 1 Tf
2.0296 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[()9()11()4(\r)1()10()4()16()-9( )12()]TJ
/F10 1 Tf
(,)Tj
4.6998 0 Td
(the)Tj
1.474 0 Td
(most)Tj
2.2054 0 Td
(virulent)Tj
3.3562 0 Td
(human)Tj
3.0784 0 Td
(malaria)Tj
3.2258 0 Td
(parasite,)Tj
-32.893 -1.2983 Td
(but)Tj
1.5533 0 Td
(represents)Tj
4.32 0 Td
(a)Tj
0.652 0 Td
(major)Tj
2.6078 0 Td
(challenge)Tj
3.9572 0 Td
(for)Tj
1.3889 0 Td
(ongoing)Tj
3.5263 0 Td
(malaria)Tj
3.2258 0 Td
(elimination)Tj
4.8472 0 Td
(efforts)Tj
2.761 0 Td
(worldwide)Tj
4.456 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(].)Tj
-32.0993 -1.2983 Td
(The)Tj
1.7858 0 Td
(Americas)Tj
4.0025 0 Td
(were)Tj
2.1259 0 Td
(the)Tj
1.4684 0 Td
(last)Tj
1.559 0 Td
(continent)Tj
4.0819 0 Td
(colonized)Tj
4.0819 0 Td
(by)Tj
1.1848 0 Td
(humans)Tj
3.4356 0 Td
(carrying)Tj
3.5603 0 Td
(malaria)Tj
3.2258 0 Td
(parasites,)Tj
3.9118 0 Td
(but)Tj
-35.6199 -1.2982 Td
(the)Tj
1.4683 0 Td
(dates)Tj
2.262 0 Td
(and)Tj
1.7348 0 Td
(routes)Tj
2.7042 0 Td
(of)Tj
1.0148 0 Td
(migration)Tj
4.201 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(New)Tj
2.0636 0 Td
(World)Tj
2.8403 0 Td
(are)Tj
1.4457 0 Td
(still)Tj
1.6384 0 Td
(debated)Tj
3.3619 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
2.5965 0 Td
(Archae-)Tj
-34.1402 -1.3039 Td
(ological)Tj
3.3165 0 Td
(evidence)Tj
3.7077 0 Td
(for)Tj
1.3889 0 Td
(infection)Tj
3.7928 0 Td
(with)Tj
1.9955 0 Td
(this)Tj
1.6725 0 Td
(parasite)Tj
3.3278 0 Td
(in)Tj
1.0375 0 Td
(indigenous,)Tj
4.8869 0 Td
(pre-Columbian)Tj
6.4062 0 Td
(populations)Tj
4.9493 0 Td
(is)Tj
-36.4816 -1.2983 Td
(currently)Tj
3.8834 0 Td
(limited)Tj
3.0727 0 Td
(to)Tj
1.0205 0 Td
(a)Tj
0.6576 0 Td
(single)Tj
2.5285 0 Td
(report)Tj
2.7099 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(antigens)Tj
3.566 0 Td
(being)Tj
2.4321 0 Td
(visualized)Tj
4.1442 0 Td
(by)Tj
1.1792 0 Td
(immunohistochem-)Tj
-29.5084 -1.2983 Td
(istry)Tj
1.9729 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(liver)Tj
1.9785 0 Td
(and)Tj
1.7348 0 Td
(spleen)Tj
2.7383 0 Td
(of)Tj
1.0148 0 Td
(South)Tj
2.5568 0 Td
(American)Tj
4.1896 0 Td
(mummies)Tj
4.2576 0 Td
(dating)Tj
2.7609 0 Td
(from)Tj
2.2054 0 Td
(3,000)Tj
2.3527 0 Td
(to)Tj
1.0205 0 Td
(600)Tj
1.6497 0 Td
(years)Tj
2.2621 0 Td
(ago)Tj
-35.2004 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
1.5987 0 Td
(Interestingly,)Tj
5.4935 0 Td
(specific)Tj
3.1918 0 Td
(antibodies)Tj
4.3596 0 Td
(failed)Tj
2.4038 0 Td
(to)Tj
1.0261 0 Td
(detect)Tj
/F15 1 Tf
2.6192 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9922 0 Td
[()9()11()4(\r)1()10()4()16()-9( )12()]TJ
/F10 1 Tf
4.473 0 Td
(antigens)Tj
3.566 0 Td
(in)Tj
1.0374 0 Td
(these)Tj
2.2564 0 Td
(same)Tj
2.2564 0 Td
(sam-)Tj
-35.2741 -1.2982 Td
(ples)Tj
1.7688 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
1.5987 0 Td
(These)Tj
2.5682 0 Td
(findings)Tj
3.4922 0 Td
(are)Tj
1.4514 0 Td
(consistent)Tj
4.2519 0 Td
(with)Tj
2.0012 0 Td
(the)Tj
1.4684 0 Td
(hypothesis)Tj
4.4674 0 Td
(that)Tj
/F15 1 Tf
1.7744 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9978 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
(,)Tj
2.5285 0 Td
(but)Tj
1.5534 0 Td
(not)Tj
/F15 1 Tf
1.576 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9922 0 Td
[()9()11()4(\r)1()10()4()16()-9( )12()]TJ
/F10 1 Tf
(,)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 408.8125 745.7952 cm
BT
/F9 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Plasmodi)Tj
4.0394 0 Td
(um)Tj
1.5874 0 Td
(vivax)Tj
/F0 1 Tf
2.459 0 Td
(genomes)Tj
4.2662 0 Td
(from)Tj
2.1969 0 Td
(the)Tj
1.5874 0 Td
(New)Tj
2.1898 0 Td
(World)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
163.5591 34.9228 m
317.7071 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Neglected)Tj
4.6417 0 Td
(Tropical)Tj
3.7135 0 Td
(Disease)Tj
3.5433 0 Td
(s)Tj
0.7158 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8197 0 Td
(1/journal.pn)Tj
5.1237 0 Td
(td.00058)Tj
3.8197 0 Td
(24)Tj
0 g
2.0905 0 Td
(July)Tj
1.9701 0 Td
(31,)Tj
1.5804 0 Td
(2017)Tj
25.3773 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(24)Tj
ET
Q
q
0.83 0.64 0.02 0 k
1 j
1 J
0 w
110.778 685.6441 m
175.8047 685.6441 l
h
f*
36 674.6457 m
48.9827 674.6457 l
h
f*
62.589 553.663 m
137.3669 553.663 l
h
f*
0 g
7.9999 0 0 7.9999 36 708.7181 cm
BT
/F12 1 Tf
1 TL
-0.025 Tc
0 0 Td
(\(2010/51835-)Tj
5.1307 0 Td
(7)Tj
0.6945 0 Td
(to)Tj
0.9496 0 Td
(MUF\),)Tj
2.5158 0 Td
(and)Tj
1.5874 0 Td
(the)Tj
1.3677 0 Td
(National)Tj
3.2386 0 Td
(Institute)Tj
-15.4843 -1.3748 Td
(of)Tj
0.9496 0 Td
(Allergy)Tj
2.7567 0 Td
(and)Tj
1.5874 0 Td
(Infectiou)Tj
3.2173 0 Td
(s)Tj
0.6449 0 Td
(Diseases)Tj
3.5292 0 Td
(\(NIAID\),)Tj
3.2669 0 Td
(National)Tj
-15.952 -1.3748 Td
(Institutes)Tj
3.6425 0 Td
(of)Tj
0.9496 0 Td
(Health)Tj
2.5725 0 Td
(\(NIH;)Tj
0.83 0.64 0.02 0 k
2.1827 0 Td
(https://www.)Tj
4.7551 0 Td
(niaid.nih.)Tj
-14.1024 -1.3748 Td
(gov/)Tj
0 g
(\),)Tj
2.374 0 Td
(USA)Tj
1.8567 0 Td
(\(International)Tj
5.1449 0 Td
(Centers)Tj
3.0473 0 Td
(of)Tj
0.9496 0 Td
(Excellenc)Tj
3.437 0 Td
(e)Tj
0.6449 0 Td
(in)Tj
-17.4544 -1.3748 Td
(Malaria)Tj
2.9338 0 Td
(Researc)Tj
2.9977 0 Td
(h)Tj
0.6945 0 Td
([ICEMR])Tj
3.4299 0 Td
(program,)Tj
3.6568 0 Td
(U19)Tj
-13.7127 -1.3748 Td
(AI089681)Tj
3.8409 0 Td
(to)Tj
0.9496 0 Td
(Joseph)Tj
2.9056 0 Td
(M.)Tj
1.1976 0 Td
(Vinetz,)Tj
2.6717 0 Td
(University)Tj
3.9118 0 Td
(of)Tj
-15.4772 -1.3748 Td
(California,)Tj
3.8976 0 Td
(San)Tj
1.6441 0 Td
(Diego\);)Tj
2.8985 0 Td
(PTR)Tj
1.8071 0 Td
(was)Tj
1.7008 0 Td
(supported)Tj
3.9969 0 Td
(by)Tj
1.1126 0 Td
(a)Tj
-17.0576 -1.3748 Td
(scholarship)Tj
4.4929 0 Td
(from)Tj
2.0126 0 Td
(CNPq,)Tj
2.5512 0 Td
(which)Tj
2.4237 0 Td
(also)Tj
1.7291 0 Td
(provided)Tj
3.4583 0 Td
(a)Tj
-16.6678 -1.3749 Td
(senior)Tj
2.5086 0 Td
(researcher)Tj
4.1457 0 Td
(scholarship)Tj
4.493 0 Td
(to)Tj
0.9496 0 Td
(MUF.)Tj
2.204 0 Td
(RMG-L)Tj
-14.3009 -1.3748 Td
(\(2010/51938-)Tj
5.1307 0 Td
(0\),)Tj
1.226 0 Td
(NFL)Tj
1.7008 0 Td
(\(2013/26928-)Tj
5.1307 0 Td
(0\),)Tj
1.226 0 Td
(and)Tj
1.5875 0 Td
(SB)Tj
-16.0017 -1.3748 Td
(\(2013/23770-)Tj
5.1307 0 Td
(6\))Tj
0.9992 0 Td
(were)Tj
2.0126 0 Td
(supporte)Tj
3.2953 0 Td
(d)Tj
0.6945 0 Td
(by)Tj
1.1197 0 Td
(scholarsh)Tj
3.6001 0 Td
(ips)Tj
-16.8521 -1.3748 Td
(from)Tj
2.0126 0 Td
(FAPESP)Tj
3.2102 0 Td
(and)Tj
1.5945 0 Td
(TCO)Tj
1.8 0 Td
(was)Tj
1.7079 0 Td
(supporte)Tj
3.2953 0 Td
(d)Tj
0.6945 0 Td
(by)Tj
1.1126 0 Td
(a)Tj
-15.4276 -1.3748 Td
(scholarship)Tj
4.4929 0 Td
(from)Tj
2.0126 0 Td
(the)Tj
1.3677 0 Td
(Coordenac)Tj
0 Tc
3.6284 0 Td
()Tj
/F14 1 Tf
-0.025 Tc
0.3614 0 Td
[( m)]TJ
/F12 1 Tf
(o)Tj
1.1126 0 Td
(de)Tj
-12.9756 -1.3748 Td
(Aperfeic)Tj
0 Tc
2.6575 0 Td
()Tj
-0.025 Tc
0.3614 0 Td
(oamento)Tj
3.4937 0 Td
(de)Tj
1.1126 0 Td
(Pessoal)Tj
3.0969 0 Td
(de)Tj
1.1197 0 Td
(N)Tj
0 Tc
0.5315 0.0212 Td
()Tj
-0.025 Tc
0.248 -0.0212 Td
(vel)Tj
1.2543 0 Td
(Superior)Tj
-13.8756 -1.3748 Td
(\(CAPES;)Tj
0.83 0.64 0.02 0 k
3.3236 0 Td
(http://www)Tj
4.1103 0 Td
(.capes.gov.br)Tj
4.9819 0 Td
(/)Tj
0 g
(\),)Tj
1.0063 0 Td
(Brazil.)Tj
2.4449 0 Td
(The)Tj
-15.867 -1.3749 Td
(funders)Tj
3.0472 0 Td
(had)Tj
1.5874 0 Td
(no)Tj
1.1693 0 Td
(role)Tj
1.6087 0 Td
(in)Tj
0.8929 0 Td
(study)Tj
2.2607 0 Td
(design,)Tj
2.8984 0 Td
(data)Tj
1.793 0 Td
(collection)Tj
-15.2576 -1.3748 Td
(and)Tj
1.5874 0 Td
(analysis,)Tj
3.4087 0 Td
(decision)Tj
3.2953 0 Td
(to)Tj
0.9496 0 Td
(publish,)Tj
3.1464 0 Td
(or)Tj
0.9993 0 Td
(preparation)Tj
4.4362 0 Td
(of)Tj
-17.8229 -1.3748 Td
(the)Tj
1.3677 0 Td
(manuscript.)Tj
/F11 1 Tf
-1.3677 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F12 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3748 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
35 0 obj
<>stream
endstream
endobj
36 0 obj
<>stream
endstream
endobj
37 0 obj
<>stream
endstream
endobj
38 0 obj
<>stream
endstream
endobj
39 0 obj
<>stream
endstream
endobj
40 0 obj
<>stream
endstream
endobj
41 0 obj
<>stream
endstream
endobj
42 0 obj
<>stream
BT
10.3394 -26.8726 Td
(exist.)Tj
ET
Q
endstream
endobj
43 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 14 0 R/Annots 45 0 R/Contents 46 0 R/TrimBox[0 0 612 792]>>
endobj
45 0 obj
[47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R 60 0 R 61 0 R 62 0 R 63 0 R 64 0 R 65 0 R 66 0 R 67 0 R 68 0 R]
endobj
47 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref005)>>
endobj
48 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref009)>>
endobj
49 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref010)>>
endobj
50 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref012)>>
endobj
51 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref007)>>
endobj
52 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref013)>>
endobj
53 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref011)>>
endobj
54 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref014)>>
endobj
55 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref001)>>
endobj
56 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref015)>>
endobj
57 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref016)>>
endobj
58 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref018)>>
endobj
59 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref011)>>
endobj
60 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref014)>>
endobj
61 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref015)>>
endobj
62 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref019)>>
endobj
63 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.g001)>>
endobj
64 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.s001)>>
endobj
65 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.ref020)>>
endobj
66 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.s001)>>
endobj
67 0 obj
<>/Border[0 0 0]/Dest(Rpntd.0005824.s001)>>
endobj
68 0 obj
<>/Border[0 0 0]/A 69 0 R>>
endobj
69 0 obj
<>
endobj
46 0 obj
[70 0 R 71 0 R 72 0 R 73 0 R 74 0 R 75 0 R 76 0 R 77 0 R 78 0 R 79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R 85 0 R 86 0 R 87 0 R 88 0 R 89 0 R 90 0 R]
endobj
70 0 obj
<>stream
q
0.83 0.64 0.02 0 k
232.2142 693.2976 m
236.9764 693.2976 l
h
f*
525.0331 693.2976 m
529.7953 693.2976 l
h
f*
322.4693 654.3496 m
331.9937 654.3496 l
h
f*
337.1528 654.3496 m
346.6772 654.3496 l
h
f*
479.3386 641.3102 m
484.1008 641.3102 l
h
f*
486.3685 641.3102 m
495.8929 641.3102 l
h
f*
203.4142 615.3449 m
212.9386 615.3449 l
h
f*
215.1496 615.3449 m
224.674 615.3449 l
h
f*
437.4425 602.3055 m
442.2047 602.3055 l
h
f*
203.4142 563.3008 m
212.9386 563.3008 l
h
f*
256.6488 537.3354 m
266.1732 537.3354 l
h
f*
271.3323 537.3354 m
280.8567 537.3354 l
h
f*
236.9764 472.3087 m
246.4441 472.3087 l
h
f*
248.7118 472.3087 m
258.2362 472.3087 l
h
f*
528.8882 297.0709 m
538.4126 297.0709 l
h
f*
540.6803 297.0709 m
550.2047 297.0709 l
h
f*
276.7181 245.0835 m
296.2772 245.0835 l
h
f*
226.9984 172.9134 m
258.1795 172.9134 l
h
f*
239.6409 159.9307 m
249.1654 159.9307 l
h
f*
380.7496 133.9087 m
446.5134 133.9087 l
h
f*
454.3937 133.9087 m
486.085 133.9087 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F10 1 Tf
1 TL
-0.004 Tc
0 0 Td
(was)Tj
1.6951 0 Td
(brought)Tj
3.4242 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.474 0 Td
(New)Tj
2.0636 0 Td
(World)Tj
2.8347 0 Td
(by)Tj
1.1848 0 Td
(early)Tj
2.1487 0 Td
(human)Tj
3.0784 0 Td
(migrations)Tj
4.5581 0 Td
(from)Tj
2.2053 0 Td
(East)Tj
1.8822 0 Td
(Asia)Tj
1.9672 0 Td
(or)Tj
1.0999 0 Td
(the)Tj
1.4683 0 Td
(Western)Tj
-32.1106 -1.3039 Td
(Pacific)Tj
2.8799 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(],)Tj
1.6044 0 Td
(but)Tj
1.5477 0 Td
(more)Tj
2.3471 0 Td
(specific)Tj
3.1861 0 Td
(molecular)Tj
4.2236 0 Td
(techniques)Tj
4.5241 0 Td
(are)Tj
1.4513 0 Td
(required)Tj
3.6397 0 Td
(to)Tj
1.0261 0 Td
(confirm)Tj
3.4413 0 Td
(them)Tj
2.2903 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(].)Tj
1.5988 0 Td
(Never-)Tj
-33.7604 -1.2982 Td
(theless,)Tj
3.084 0 Td
(present-day)Tj
4.9323 0 Td
(New)Tj
2.0636 0 Td
(World)Tj
2.8403 0 Td
(populations)Tj
4.9492 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9922 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3073 0 Td
(appear)Tj
2.9197 0 Td
(to)Tj
1.0205 0 Td
(be)Tj
1.1508 0 Td
(more)Tj
2.3414 0 Td
(closely)Tj
2.8857 0 Td
(related)Tj
2.9423 0 Td
(to)Tj
-35.4441 -1.2983 Td
(extant)Tj
2.6872 0 Td
(African)Tj
3.2371 0 Td
(and)Tj
1.7291 0 Td
(South)Tj
2.5569 0 Td
(Asian)Tj
2.5115 0 Td
(parasites)Tj
3.6906 0 Td
(and)Tj
1.7292 0 Td
(now)Tj
1.9615 0 Td
(extinct)Tj
2.9424 0 Td
(European)Tj
4.1215 0 Td
(lineages)Tj
3.3846 0 Td
(than)Tj
2.0296 0 Td
(to)Tj
1.0261 0 Td
(East)Tj
-33.6073 -1.2983 Td
(Asian)Tj
2.5114 0 Td
(and)Tj
1.7348 0 Td
(Melanesian)Tj
4.7962 0 Td
(strains)Tj
2.863 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(],)Tj
3.5433 0 Td
(consistent)Tj
4.2576 0 Td
(with)Tj
1.9956 0 Td
(much)Tj
2.5228 0 Td
(more)Tj
2.3414 0 Td
(recent)Tj
2.7042 0 Td
(parasite)Tj
3.3279 0 Td
(migrations)Tj
-32.5982 -1.3039 Td
(with)Tj
1.9955 0 Td
(European)Tj
4.1216 0 Td
(conquerors)Tj
4.7678 0 Td
(and)Tj
1.7291 0 Td
(African)Tj
3.2372 0 Td
(slaves)Tj
2.5001 0 Td
(during)Tj
2.9254 0 Td
(the)Tj
1.4683 0 Td
(colonial)Tj
3.4016 0 Td
(era)Tj
1.4513 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(].)Tj
-26.4017 -1.2982 Td
(Clinical)Tj
3.3165 0 Td
(isolates)Tj
3.1181 0 Td
(from)Tj
2.2053 0 Td
(Brazil)Tj
2.5342 0 Td
(are)Tj
1.4456 0 Td
(underrepresented)Tj
7.3134 0 Td
(in)Tj
1.0318 0 Td
(global)Tj
2.6248 0 Td
(genomic)Tj
3.6794 0 Td
(analyses)Tj
3.4809 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
-33.9531 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
3.2541 0 Td
(However,)Tj
4.0762 0 Td
(this)Tj
1.6724 0 Td
(country)Tj
3.3506 0 Td
(contributes)Tj
4.7565 0 Td
(37%)Tj
1.9275 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(malaria)Tj
3.2259 0 Td
(burden)Tj
3.1237 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(Americas,)Tj
4.2293 0 Td
(a)Tj
-34.6051 -1.3039 Td
(region)Tj
2.8006 0 Td
(with)Tj
1.9955 0 Td
(over)Tj
1.9786 0 Td
(20)Tj
1.1735 0 Td
(million)Tj
3.1181 0 Td
(people)Tj
2.8573 0 Td
(at)Tj
0.9525 0 Td
(high)Tj
2.0069 0 Td
(risk)Tj
1.7178 0 Td
(of)Tj
1.0148 0 Td
(infection)Tj
3.787 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(].)Tj
1.6044 0 Td
(Obtaining)Tj
4.303 0 Td
(large)Tj
2.16 0 Td
(amounts)Tj
3.7134 0 Td
(of)Tj
-35.1834 -1.2983 Td
(host-free)Tj
/F15 1 Tf
3.77 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(DNA)Tj
2.3754 0 Td
(from)Tj
2.2054 0 Td
(clinical)Tj
3.0784 0 Td
(samples)Tj
3.3845 0 Td
(from)Tj
2.2054 0 Td
(Brazil)Tj
2.5285 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(a)Tj
0.6519 0 Td
(major)Tj
2.6079 0 Td
(challenge)Tj
3.9571 0 Td
(for)Tj
-33.732 -1.2983 Td
(genome)Tj
3.4072 0 Td
(sequencing)Tj
4.7168 0 Td
(projects,)Tj
3.6 0 Td
(because)Tj
3.3222 0 Td
(\(a\))Tj
1.3322 0 Td
(blood-stage)Tj
4.8472 0 Td
(parasite)Tj
3.3279 0 Td
(densities)Tj
3.6964 0 Td
(are)Tj
1.4513 0 Td
(typically)Tj
3.5603 0 Td
(very)Tj
1.9219 0 Td
(low)Tj
-35.1834 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(],)Tj
2.0749 0 Td
(\(b\))Tj
1.406 0 Td
(clinical)Tj
3.0784 0 Td
(blood)Tj
2.5115 0 Td
(samples)Tj
3.3845 0 Td
(are)Tj
1.4513 0 Td
(heavily)Tj
3.0274 0 Td
(contaminated)Tj
5.7486 0 Td
(with)Tj
1.9956 0 Td
(human)Tj
3.0784 0 Td
(DNA)Tj
2.3754 0 Td
(from)Tj
2.2111 0 Td
(leukocytes,)Tj
-32.3431 -1.2982 Td
(and)Tj
1.7291 0 Td
(\(c\))Tj
1.3209 0 Td
(methods)Tj
3.685 0 Td
(for)Tj
1.389 0 Td
(long-term)Tj
/F15 1 Tf
4.2519 0 Td
[()12()]TJ
1.0148 0 Td
[(\n)-13()14()7()20()]TJ
/F10 1 Tf
2.041 0 Td
(propagation)Tj
5.091 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(are)Tj
1.4456 0 Td
(neither)Tj
3.0841 0 Td
(practical)Tj
3.634 0 Td
(nor)Tj
1.6441 0 Td
(widely)Tj
-34.6448 -1.2983 Td
(reproducible)Tj
5.3234 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
3.5376 0 Td
(Here,)Tj
2.4208 0 Td
(we)Tj
1.3209 0 Td
(combined)Tj
4.2349 0 Td
(an)Tj
1.1963 0 Td
(improved)Tj
4.0988 0 Td
(sample)Tj
3.0161 0 Td
(preparation)Tj
4.8982 0 Td
(strategy,)Tj
3.5433 0 Td
(for)Tj
1.3776 0 Td
(reduc-)Tj
-34.9679 -1.3039 Td
(ing)Tj
1.5023 0 Td
(human)Tj
3.0727 0 Td
(DNA)Tj
2.3811 0 Td
(contamination)Tj
6.1172 0 Td
(and)Tj
1.7347 0 Td
(increasing)Tj
4.3257 0 Td
(target)Tj
2.5115 0 Td
(parasite's)Tj
3.9061 0 Td
(DNA)Tj
2.3754 0 Td
(yield,)Tj
2.3641 0 Td
(with)Tj
1.9956 0 Td
(next-genera-)Tj
-32.2864 -1.2983 Td
(tion)Tj
1.8425 0 Td
(genome)Tj
3.4072 0 Td
(re-sequencing)Tj
5.8563 0 Td
(to)Tj
1.0262 0 Td
(examine)Tj
3.5999 0 Td
(a)Tj
0.6577 0 Td
(local)Tj
2.0749 0 Td
(population)Tj
4.5921 0 Td
(of)Tj
/F15 1 Tf
1.0148 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(Amazon)Tj
3.6283 0 Td
(Basin)Tj
-34.6731 -1.2982 Td
(of)Tj
1.0147 0 Td
(Brazil.)Tj
2.7553 0 Td
(Our)Tj
1.8652 0 Td
(nine)Tj
2.0069 0 Td
(high-quality)Tj
5.0967 0 Td
(genome)Tj
3.4072 0 Td
(sequences)Tj
4.2179 0 Td
(were)Tj
2.1203 0 Td
(compared)Tj
4.2463 0 Td
(to)Tj
1.0261 0 Td
(those)Tj
2.3358 0 Td
(previously)Tj
-30.0924 -1.2983 Td
(obtained)Tj
3.7303 0 Td
(from)Tj
2.2054 0 Td
(four)Tj
1.9162 0 Td
(countries)Tj
3.9458 0 Td
(\(Brazil)Tj
2.8743 0 Td
([BRA],)Tj
3.0104 0 Td
(Peru)Tj
2.1033 0 Td
([PER],)Tj
2.8743 0 Td
(Colombia)Tj
4.1725 0 Td
([COL],)Tj
3.0728 0 Td
(and)Tj
1.7291 0 Td
(Mexico)Tj
-31.6344 -1.3039 Td
([MEX]\))Tj
3.3561 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(])Tj
3.0274 0 Td
(to)Tj
1.0262 0 Td
(reveal)Tj
2.5681 0 Td
(local)Tj
2.0807 0 Td
(and)Tj
1.7291 0 Td
(regional)Tj
3.4809 0 Td
(patterns)Tj
3.4753 0 Td
(of)Tj
1.0148 0 Td
(diversity)Tj
3.6453 0 Td
(and)Tj
1.7291 0 Td
(differentiation)Tj
5.9811 0 Td
(in)Tj
1.0318 0 Td
(extant)Tj
/F15 1 Tf
-34.1459 -1.2983 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9921 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(populations)Tj
4.9492 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.474 0 Td
(New)Tj
2.0636 0 Td
(World.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 431.2629 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Methods)Tj
/F0 1 Tf
0 -1.4976 Td
(Ethics)Tj
2.9149 0 Td
(statement)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 396.2834 cm
BT
/F10 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Study)Tj
2.4944 0 Td
(protocols)Tj
3.9515 0 Td
(were)Tj
2.1203 0 Td
(approved)Tj
3.9628 0 Td
(by)Tj
1.1735 0 Td
(the)Tj
1.4627 0 Td
(Institutional)Tj
5.159 0 Td
(Review)Tj
3.0841 0 Td
(Board)Tj
2.6362 0 Td
(of)Tj
1.0091 0 Td
(the)Tj
1.4627 0 Td
(Institute)Tj
3.5773 0 Td
(of)Tj
1.0035 0 Td
(Biomedical)Tj
-33.0971 -1.3039 Td
(Sciences,)Tj
3.7927 0 Td
(University)Tj
4.3993 0 Td
(of)Tj
1.0148 0 Td
(S)Tj
/F16 1 Tf
[()]TJ
/F10 1 Tf
(o)Tj
1.6384 0 Td
(Paulo,)Tj
2.7156 0 Td
(Brazil)Tj
2.5342 0 Td
(\(936/CEP,)Tj
4.3313 0 Td
(2010)Tj
2.1259 0 Td
(and)Tj
1.7292 0 Td
(1183/CEPSH,)Tj
5.7146 0 Td
(2014\).)Tj
2.6872 0 Td
(Written)Tj
-32.6832 -1.2982 Td
(informed)Tj
3.9741 0 Td
(consent)Tj
3.3279 0 Td
(was)Tj
1.7007 0 Td
(obtained)Tj
3.7304 0 Td
(from)Tj
2.2053 0 Td
(all)Tj
1.1452 0 Td
(patients.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 341.1212 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Sample)Tj
3.5716 0 Td
(collection)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 324.1133 cm
BT
/F10 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Parasite)Tj
3.3675 0 Td
(samples)Tj
3.3845 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(between)Tj
3.5263 0 Td
(November)Tj
4.4617 0 Td
(2012)Tj
2.1203 0 Td
(and)Tj
1.7348 0 Td
(June)Tj
2.0466 0 Td
(2013)Tj
2.126 0 Td
(in)Tj
1.0318 0 Td
(eastern)Tj
3.0841 0 Td
(Acre)Tj
2.1203 0 Td
(and)Tj
-34.8659 -1.2982 Td
(southern)Tj
3.7813 0 Td
(Amazonas,)Tj
4.6488 0 Td
(Amazon)Tj
3.6284 0 Td
(Basin)Tj
2.4094 0 Td
(of)Tj
1.0148 0 Td
(Brazil,)Tj
2.7552 0 Td
(close)Tj
2.1827 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(borders)Tj
3.2881 0 Td
(with)Tj
1.9956 0 Td
(Peru)Tj
2.1033 0 Td
(and)Tj
1.7291 0 Td
(Bolivia.)Tj
-32.0312 -1.2983 Td
(Malaria)Tj
3.2995 0 Td
(epidemiology)Tj
5.6465 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4684 0 Td
(study)Tj
2.3924 0 Td
(sites)Tj
1.9332 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.1203 0 Td
(characterized)Tj
5.5615 0 Td
(in)Tj
1.0318 0 Td
(detail)Tj
2.4208 0 Td
(elsewhere)Tj
4.0988 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
-32.5471 -1.3039 Td
(Venous)Tj
3.2824 0 Td
(blood)Tj
2.5115 0 Td
(samples)Tj
3.3846 0 Td
(\(10)Tj
1.5137 0 Td
(ml\))Tj
1.6214 0 Td
(were)Tj
2.1259 0 Td
(collected)Tj
3.7361 0 Td
(from)Tj
2.2053 0 Td
(eight)Tj
2.2054 0 Td
(adult)Tj
2.2507 0 Td
(patients)Tj
3.3675 0 Td
(attending)Tj
4.0365 0 Td
(malaria)Tj
3.2258 0 Td
(clin-)Tj
-35.4668 -1.2983 Td
(ics)Tj
1.2699 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(town)Tj
2.2563 0 Td
(of)Tj
1.0148 0 Td
(Acrela)Tj
0 Tc
2.1714 0.0511 Td
()Tj
-0.004 Tc
0.4138 -0.0511 Td
(ndia,)Tj
2.2224 0 Td
(Acre)Tj
2.1203 0 Td
(\(943')Tj
2.5965 0 Td
(S,)Tj
0.9184 0 Td
(6653')Tj
2.7326 0 Td
(W\),)Tj
1.7518 0 Td
(and)Tj
1.7291 0 Td
(one)Tj
1.7008 0 Td
(adult)Tj
2.2507 0 Td
(patient)Tj
3.0104 0 Td
(living)Tj
2.4661 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
-34.1686 -1.2982 Td
(farming)Tj
3.4128 0 Td
(settlement)Tj
4.3597 0 Td
(of)Tj
1.0148 0 Td
(Remansinho,)Tj
5.5048 0 Td
(Amazonas)Tj
4.4221 0 Td
(\(940')Tj
2.5908 0 Td
(S-943')Tj
3.0841 0 Td
(S,)Tj
0.9184 0 Td
(6652')Tj
2.7326 0 Td
(W)Tj
1.1849 0 Td
(-6659')Tj
3.0784 0 Td
(W\),)Tj
1.7518 0 Td
(120)Tj
1.6497 0 Td
(km)Tj
-35.7049 -1.2983 Td
(east)Tj
1.7347 0 Td
(of)Tj
1.0148 0 Td
(Acrela)Tj
0 Tc
2.1714 0.051 Td
()Tj
-0.004 Tc
0.4138 -0.051 Td
(ndia)Tj
1.9956 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1)Tj
0 g
(\).)Tj
/F15 1 Tf
1.2586 0 Td
[()]TJ
/F10 1 Tf
(.)Tj
/F15 1 Tf
0.9978 0 Td
[(\n)-13()15(\n)3()]TJ
/F10 1 Tf
2.3074 0 Td
(infection)Tj
3.787 0 Td
(was)Tj
1.7008 0 Td
(diagnosed)Tj
4.2803 0 Td
(by)Tj
1.1849 0 Td
(on-site)Tj
2.9763 0 Td
(microscopy)Tj
4.8699 0 Td
(and)Tj
1.7292 0 Td
(later)Tj
-34.2423 -1.3039 Td
(confirmed)Tj
4.3936 0 Td
(by)Tj
1.1849 0 Td
(qPCR)Tj
2.5682 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(below.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 203.9811 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Leukocyte)Tj
4.7244 0 Td
(depletion)Tj
4.2189 0 Td
(and)Tj
1.8662 0 Td
(sample)Tj
3.4063 0 Td
(cryopreservation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 186.9732 cm
BT
/F10 1 Tf
1 TL
-0.004 Tc
0 0 Td
(We)Tj
1.61 0 Td
(adapted)Tj
3.3789 0 Td
(BioR)Tj
2.1997 0 Td
(01)Tj
1.1678 0 Td
(Plus)Tj
1.9162 0 Td
(leukocyte-depletion)Tj
8.1298 0 Td
(filters)Tj
2.4774 0 Td
(\(Fresenius)Tj
4.371 0 Td
(Kabi,)Tj
2.3244 0 Td
(Bad)Tj
1.7631 0 Td
(Homburg,)Tj
4.4107 0 Td
(Ger-)Tj
-33.749 -1.2982 Td
(many;)Tj
0.83 0.64 0.02 0 k
2.6985 0 Td
(S1A)Tj
1.8595 0 Td
(Fig)Tj
0 g
(\))Tj
1.8199 0 Td
(to)Tj
1.0261 0 Td
(process)Tj
3.1805 0 Td
(10)Tj
1.1735 0 Td
(to)Tj
1.0261 0 Td
(50-ml)Tj
2.5852 0 Td
(volumes)Tj
3.5603 0 Td
(of)Tj
1.0148 0 Td
(venous)Tj
3.0444 0 Td
(blood)Tj
2.5115 0 Td
(in)Tj
1.0374 0 Td
(our)Tj
1.6271 0 Td
(field)Tj
1.9729 0 Td
(laboratory)Tj
4.3483 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
-35.5235 -1.2983 Td
(Amazon)Tj
3.6226 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
2.0806 0 Td
(The)Tj
1.7802 0 Td
(per-unit)Tj
3.5262 0 Td
(cost)Tj
1.8085 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2564 0 Td
(filters)Tj
2.4718 0 Td
(in)Tj
1.0375 0 Td
(Brazil)Tj
2.5285 0 Td
(is)Tj
0.8503 0 Td
(around)Tj
3.1351 0 Td
(US$)Tj
1.9106 0 Td
(25.)Tj
1.3946 0 Td
(We)Tj
1.6101 0 Td
(first)Tj
1.8028 0 Td
(cut)Tj
1.474 0 Td
(off)Tj
-34.3046 -1.2982 Td
(under)Tj
2.6191 0 Td
(sterile)Tj
2.6079 0 Td
(conditions,)Tj
4.7055 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.6519 0 Td
(scissor,)Tj
3.0954 0 Td
(the)Tj
1.474 0 Td
(tubing)Tj
2.8347 0 Td
(that)Tj
1.7745 0 Td
(connects)Tj
3.753 0 Td
(the)Tj
1.474 0 Td
(filtering)Tj
3.3959 0 Td
(device)Tj
2.7382 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
-34.1459 -1.304 Td
(400-ml)Tj
3.0613 0 Td
(blood)Tj
2.5172 0 Td
(storage)Tj
3.0784 0 Td
(bag)Tj
1.6271 0 Td
(and)Tj
1.7291 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(adapter)Tj
3.2258 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1B)Tj
2.0919 0 Td
(and)Tj
1.7292 0 Td
(S1C)Tj
1.8368 0 Td
(Fig)Tj
0 g
(\).)Tj
0.83 0.64 0.02 0 k
2.0466 0 Td
(S1D)Tj
1.9049 0 Td
(Fig)Tj
0 g
1.4796 0 Td
(shows)Tj
2.6589 0 Td
(how)Tj
1.9389 0 Td
(the)Tj
1.474 0 Td
(filter-)Tj
-34.8942 -1.2982 Td
(ing)Tj
1.5023 0 Td
(device)Tj
2.7383 0 Td
(was)Tj
1.7007 0 Td
(used)Tj
2.058 0 Td
(in)Tj
1.0374 0 Td
(a)Tj
0.652 0 Td
(laminar)Tj
3.3392 0 Td
(flow)Tj
1.9446 0 Td
(hood;)Tj
2.5171 0 Td
(briefly,)Tj
3.0047 0 Td
(a)Tj
0.652 0 Td
(10-ml)Tj
2.5852 0 Td
(syringe)Tj
3.1124 0 Td
(was)Tj
1.7008 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(apply)Tj
2.3925 0 Td
(blood)Tj
-34.0212 -1.2983 Td
(treated)Tj
2.9933 0 Td
(with)Tj
2.0013 0 Td
(acid)Tj
1.8708 0 Td
(citrate)Tj
2.7383 0 Td
(dextrose)Tj
3.5943 0 Td
(anticoagulant,)Tj
5.879 0 Td
(while)Tj
2.3697 0 Td
(a)Tj
0.652 0 Td
(second)Tj
3.0104 0 Td
(10-ml)Tj
2.5908 0 Td
(syringe)Tj
3.1068 0 Td
(was)Tj
1.7008 0 Td
(adapted)Tj
3.3845 0 Td
(to)Tj
-35.892 -1.3039 Td
(the)Tj
1.4683 0 Td
(end)Tj
1.7178 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(remaining)Tj
4.3653 0 Td
(tubing)Tj
2.829 0 Td
(to)Tj
1.0261 0 Td
(recover)Tj
3.1975 0 Td
(the)Tj
1.4683 0 Td
(filtered,)Tj
3.2938 0 Td
(leucocyte-depleted)Tj
7.6819 0 Td
(material,)Tj
3.7417 0 Td
(which)Tj
2.6475 0 Td
(was)Tj
-35.926 -1.2983 Td
(transferred)Tj
4.6374 0 Td
(to)Tj
1.0261 0 Td
(50-ml)Tj
2.5852 0 Td
(sterile)Tj
2.6135 0 Td
(centrifuge)Tj
4.2577 0 Td
(tubes.)Tj
2.5625 0 Td
(No)Tj
1.4626 0 Td
(priming)Tj
3.4753 0 Td
(with)Tj
1.9956 0 Td
(saline)Tj
2.5001 0 Td
(was)Tj
1.7008 0 Td
(required.)Tj
3.8664 0 Td
(After)Tj
2.2847 0 Td
(the)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 408.8125 745.7952 cm
BT
/F9 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Plasmodi)Tj
4.0394 0 Td
(um)Tj
1.5874 0 Td
(vivax)Tj
/F0 1 Tf
2.459 0 Td
(genomes)Tj
4.2662 0 Td
(from)Tj
2.1969 0 Td
(the)Tj
1.5874 0 Td
(New)Tj
2.1898 0 Td
(World)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
163.5591 34.9228 m
317.7071 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Neglected)Tj
4.6417 0 Td
(Tropical)Tj
3.7135 0 Td
(Disease)Tj
3.5433 0 Td
(s)Tj
0.7158 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8197 0 Td
(1/journal.pn)Tj
5.1237 0 Td
(td.00058)Tj
3.8197 0 Td
(24)Tj
0 g
2.0905 0 Td
(July)Tj
1.9701 0 Td
(31,)Tj
1.5804 0 Td
(2017)Tj
25.3773 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
71 0 obj
<>stream
endstream
endobj
72 0 obj
<>stream
endstream
endobj
73 0 obj
<>stream
endstream
endobj
74 0 obj
<>stream
endstream
endobj
75 0 obj
<>stream
endstream
endobj
76 0 obj
<>stream
endstream
endobj
77 0 obj
<>stream
endstream
endobj
78 0 obj
<>stream
endstream
endobj
79 0 obj
<>stream
endstream
endobj
80 0 obj
<>stream
endstream
endobj
81 0 obj
<>stream
endstream
endobj
82 0 obj
<>stream
endstream
endobj
83 0 obj
<>stream
endstream
endobj
84 0 obj
<>stream
endstream
endobj
85 0 obj
<>stream
endstream
endobj
86 0 obj
<>stream
endstream
endobj
87 0 obj
<>stream
endstream
endobj
88 0 obj
<>stream
endstream
endobj
89 0 obj
<>stream
endstream
endobj
90 0 obj
<>stream
BT
66.402 0 Td
(24)Tj
ET
Q
endstream
endobj
91 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 14 0 R/Annots 94 0 R/Contents 95 0 R/TrimBox[0 0 612 792]>>
endobj
93 0 obj
<>stream
x^@"{p(w(VIFiyBP`ŝR\I}f7{+s|gp]=w y晝wyM7ݨY/lo7GS جNCot'@fmVy $ʜp
5^Ye!!!!!!! !NTo\ʿۺ=&{)'ʳm)M$L2s7oM=SN9e5N1L3M5)F^N:iC=c'W[
%kYz!!!!!!!!-BE#7}Rzo4x7_~mFs<0xqC 4'LSb- yS0~g}vV0ݖYfK.dذa7xcCc\t;O9V[2V$n']Q~^zi;2dʼr1bonݺ.xw>{ﭷޒ@7I\WW^yemIg?첋mS!!!!!!!!f zzxFCKQ%sO.}isnurTZrfȑ]wU03(_w?vigmu]c^~W~;ò.;묳vڕJo:[UETQMdD#|{^x3Xoy'/N[|k./?Opt@@@@@@@@tDtb8QOO>?9Ikr-CsTG@{.H}[W\ь\S
1uACϧj*OᇺkT}q4dX`:U_nuh>Gqga:_~Y\|$g馛 7qS4{[oF?;uoJ@@@@@@@@ @HqYg#