Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.

Bossé, Janine T; Li, Yanwen; Angen, Øystein; Weinert, Lucy A; Chaudhuri, Roy R; Holden, Matthew T; Williamson, Susanna M; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; +3 more... Rycroft, Andrew N; Langford, Paul R; BRaDP1T consortium; (2014) Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. Journal of clinical microbiology, 52 (7). pp. 2380-2385. ISSN 0095-1137 DOI: https://doi.org/10.1128/JCM.00685-14

Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

Additional Information

Item Type	Article
Faculty and Department	Faculty of Infectious and Tropical Diseases > Department of Infection Biology
Research Centre	?? 209951 ??
PubMed ID	24759717
ISI	339279700015
Related URLs	OA Location