A dam mutant of Yersinia pestis is attenuated and induces protection against plague.
Robinson, Victoria L;
Oyston, Petra CF;
Titball, Richard W;
(2005)
A dam mutant of Yersinia pestis is attenuated and induces protection against plague.
FEMS microbiology letters, 252 (2).
pp. 251-256.
ISSN 0378-1097
DOI: https://doi.org/10.1016/j.femsle.2005.09.001
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We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of dam inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria.
