A kinetoplast DNA minicircle probe (B18) specific for the Leishmania subgenus Viannia.
Gibson, LJ;
McCann, SHE;
Brewster, S;
Barker, DC;
(2002)
A kinetoplast DNA minicircle probe (B18) specific for the Leishmania subgenus Viannia.
Transactions of the Royal Society of Tropical Medicine and Hygiene, 96 Sup (SUPPL.).
S71-S73.
ISSN 0035-9203
DOI: https://doi.org/10.1016/s0035-9203(02)90054-4
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This paper reports the development, sequence and specificity of probe B18, which hybridizes only to the kinetoplast deoxyribonucleic acid (kDNA) minicircle of Leishmania species of the subgenus Viannia. This probe was developed in 1985 and has been used extensively since, on whole kDNA, Leishmania dot-blots and kDNA minicircles amplified by the polymerase chain reaction.
