Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population.
Wang, Xuan-Yi;
Ansaruzzaman, M;
Vaz, Raul;
Mondlane, Catarina;
Lucas, Marcelino ES;
von Seidlein, Lorenz;
Deen, Jacqueline L;
Ampuero, Sonia;
Puri, Mahesh;
Park, Taesung;
+6 more...Nair, GB;
Clemens, John D;
Chaignat, Claire-Lise;
Rajerison, Minoarisoa;
Nato, Farida;
Fournier, Jean-Michel;
(2006)
Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population.
BMC infectious diseases, 6 (1).
17-.
ISSN 1471-2334
DOI: https://doi.org/10.1186/1471-2334-6-17
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BACKGROUND: Early detection of cholera outbreaks is crucial for the implementation of the most appropriate control strategies. METHODS: The performance of an immunochromatographic dipstick test (Institute Pasteur, Paris, France) specific for Vibrio cholerae O1 was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season (January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture. RESULTS: The overall sensitivity and specificity of the rapid test compared to culture were 95% (95% confidence interval [CI]: 91%-99%) and 89% (95% CI: 86%-93%), respectively. After stratification by type of sample (rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%. CONCLUSION: This one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed.
