Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting


Imade, GE; Badung, B; Pam, S; Agbaji, O; Egah, D; Sagay, AS; Sankale, JL; Kapiga, S; Idoko, J; Kanki, P; (2005) Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting. Clinical and diagnostic laboratory immunology, 12 (1). pp. 224-7. ISSN 1071-412X DOI: https://doi.org/10.1128/CDLI.12.1.224-227.2005

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Abstract

We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

Item Type: Article
Keywords: Adult, Automation, CD4 Lymphocyte Count/economics/*methods, Flow Cytometry/economics/*methods, Humans, *Immunomagnetic Separation/economics/methods, Nigeria, Reproducibility of Results, Sensitivity and Specificity, Adult, Automation, CD4 Lymphocyte Count, economics, methods, Comparative Study, Flow Cytometry, economics, methods, Humans, Immunomagnetic Separation, economics, methods, Nigeria, Reproducibility of Results, Sensitivity and Specificity
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
Research Centre: Centre for Global Non-Communicable Diseases (NCDs)
PubMed ID: 15643012
Web of Science ID: 227271800033
URI: http://researchonline.lshtm.ac.uk/id/eprint/9897

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