[Ligase chain reaction testing of pooled urine specimens to diagnose Chlamydia trachomatis infection]


Sanchez-Aleman, MA; Gutierrez, JP; Bertozzi, SM; Frontela-Noda, M; Guerrero-Lemus, V; Conde-Gonzalez, CJ; (2005) [Ligase chain reaction testing of pooled urine specimens to diagnose Chlamydia trachomatis infection]. Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 57 (4). pp. 548-54. ISSN 0034-8376

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Abstract

OBJECTIVE: The purpose of this study was to assess the utility and validity of pooling urine samples for molecular diagnosis of Chlamydia trachomatis infection. MATERIAL AND METHODS: Of 1,220 urine samples collected from Mexican female and male adolescents, 305 pools were composed of fourth individual samples each, based on a calculation of optimal pool size. These were processed by ligase chain reaction (LCR) for the detection of C. trachomatis. Positive and gray-zone pools were reanalyzed individually. Cost savings were calculated comparing actual costs of testing to the cost that would have been incurred testing all 1,220 samples individually. RESULTS: Pools results were: 56 positive, 19 gray-zones and 230 negative. Following individual retesting of positive and gray-zone pools, 59 cases of C. trachomatis infection were identified (4.8% prevalence). Thus, a total of 601 LCR tests were performed, for a 50.4% savings considering only the direct cost of the test. CONCLUSIONS: Our experience shows that sample pooling is both a reliable and convenient tool for CT surveillance in our setting. It should be considered in other similar settings where limited resources constraint surveillance of STIs.

Item Type: Article
Keywords: Adolescent, Adult, Chlamydia Infections/ diagnosis/epidemiology/microbiology/urine, Chlamydia trachomatis/ isolation & purification, Cost Savings, Cost-Benefit Analysis, Costs and Cost Analysis, DNA, Bacterial/ urine, Female, Humans, Ligase Chain Reaction/economics/methods, Male, Mass Screening/economics/methods, Mexico/epidemiology, Population Surveillance/methods, Prevalence, Specimen Handling/economics/ methods, Urine/ microbiology, Adolescent, Adult, Chlamydia Infections, diagnosis, epidemiology, microbiology, urine, Chlamydia trachomatis, isolation & purification, Cost Savings, Cost-Benefit Analysis, Costs and Cost Analysis, DNA, Bacterial, urine, Female, Humans, Ligase Chain Reaction, economics, methods, Male, Mass Screening, economics, methods, Mexico, epidemiology, Population Surveillance, methods, Prevalence, Specimen Handling, economics, methods, Urine, microbiology
Faculty and Department: Faculty of Public Health and Policy > Dept of Global Health and Development
PubMed ID: 16315639
Web of Science ID: 233075100008
URI: http://researchonline.lshtm.ac.uk/id/eprint/8846

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