A 55 kDa hypothetical membrane protein is an iron-regulated virulence factor of Francisella tularensis subsp novicida U 112


Milne, TS; Michell, SL; Diaper, H; Wikstroem, P; Svensson, K; Oyston, PCF; Titball, RW; (2007) A 55 kDa hypothetical membrane protein is an iron-regulated virulence factor of Francisella tularensis subsp novicida U 112. Journal of medical microbiology, 56 (10). pp. 1268-1276. ISSN 0022-2615 DOI: 10.1099/jmm.0.47190-0

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Abstract

Iron is an important nutritional requirement for bacteria due to its conserved role in many essential metabolic processes. As a consequence of the lack of freely available iron in the mammalian host, bacteria upregulate a range of virulence factors during infection. Transcriptional analysis of Francisella tularensis subsp. novicida U1 12 grown in iron-deficient medium identified 21 genes upregulated in response to this condition, four of which were attributed to a siderophore operon. In addition, a novel iron-regulated gene, FTT-0025, was identified which is part of this operon and encodes a 55 kDa hypothetical membrane protein. When grown on chrome azurol S agar, the F tularensis subsp. novicida U112 Delta FTT0025 mutant produced an increased reaction zone compared with the wild-type, suggesting that siderophore production was unaffected but that the bacteria may have a deficiency in their ability to re-sequester this iron-binding molecule. Furthermore, the Delta FTT0025 mutant was attenuated in a BALB/c mouse model of infection relative to wild-type F. tularensis subsp. novicida U112.

Item Type: Article
Keywords: Animals, Bacterial Proteins, chemistry, genetics, physiology, Francisella tularensis, genetics, pathogenicity, Gene Deletion, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Iron, metabolism, Membrane Proteins, chemistry, genetics, physiology, Mice, Mice, Inbred BALB C, Molecular Weight, Oligonucleotide Array Sequence Analysis, Survival Analysis, Tularemia, microbiology, Virulence Factors, chemistry, genetics, physiology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 17893160
Web of Science ID: 250538100002
URI: http://researchonline.lshtm.ac.uk/id/eprint/8585

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