Polysaccharides and virulence of Burkholderia pseudomallei

Sarkar-Tyson, M; Thwaite, JE; Harding, SV; Smither, SJ; Oyston, PCF; Atkins, TP; Titball, RW; (2007) Polysaccharides and virulence of Burkholderia pseudomallei. Journal of medical microbiology, 56 (8). pp. 1005-1010. ISSN 0022-2615 DOI: https://doi.org/10.1099/jmm.0.47043-0

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Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease of humans and animals. Gene clusters which encode capsular polysaccharide (type I O-PS) and LIPS (type 11 O-PS), both of which play roles in virulence, have previously been identified. Here, the identification of two further putative clusters, type III O-PS and type IV O-PS, is reported. Mice challenged with type III O-PS or type IV O-PS mutants showed increased mean times to death (7.8 and 11.6 days) compared to those challenged with wild-type B. pseudomallei (3 days). To investigate the possible roles of polysaccharides in protection, mice were immunized with killed cells of wild-type B. pseudomallei or killed cells of B. pseudomallei with mutations in the 0 antigen, capsular polysaccharide, type III O-PS or type IV O-PS gene clusters. Immunization with all polysaccharide mutant strains resulted in delayed time to death compared to the naive controls, following challenge with wild-type B. pseudomallei strain K96243. However, immunization with killed polysaccharide mutant strains conferred different degrees of protection, demonstrating the immunological importance of the polysaccharide clusters on the surface of B. pseudomallei.

Item Type: Article
Keywords: Animals, Bacterial Capsules, chemistry, genetics, Bacterial Vaccines, administration & dosage, Burkholderia Infections, microbiology, prevention & control, Burkholderia pseudomallei, genetics, immunology, pathogenicity, Female, Genes, Bacterial, genetics, Genomic Islands, physiology, Immunization, Secondary, Injections, Intraperitoneal, Mice, Mice, Inbred BALB C, Multigene Family, genetics, O Antigens, genetics, Operon, Point Mutation, Polysaccharides, Bacterial, physiology, Vaccination, Virulence, Virulence Factors, physiology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 17644705
Web of Science ID: 248821300001
URI: http://researchonline.lshtm.ac.uk/id/eprint/8584


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