Chlamydia trachomatis variant not detected by plasmid based nucleic acid amplitication tests: molecular characterisation and failure of single dose azithromycin


Magbanua, JPV; Goh, BT; Michel, CE; Aguirre-Andreasen, A; Alexander, S; Ushiro-Lumb, I; Ison, C; Lee, H; (2007) Chlamydia trachomatis variant not detected by plasmid based nucleic acid amplitication tests: molecular characterisation and failure of single dose azithromycin. Sexually transmitted infections, 83 (4). ISSN 1368-4973 DOI: 10.1136/sti.2007.026435

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Abstract

Objective: To characterise a Chlamydio trachomatis variant strain from a patient with non-gonococcal urethritis (NGU) whose first void urine (FVU) displayed discrepant C trachomatis test results and describe the clinical response to treatment. Methods: The FVU specimen was assayed with an immune based Chlamydio Rapid Test (CRT) and various nucleic acid amplification tests (NAATs) to establish C trachomatis infection. Sequencing of the major outer membrane protein gene (omp I also known as ompA) was undertaken to identify the serovar of the variant strain. Polymerase chain reaction (PCR) analysis was also conducted to determine whether the strain harboured deletions in the cryptic plasmid or was plasmid free. Results: The FVU specimen was strongly reactive in CRT but negative with the plasmid based Amplicor PCR (Roche) and ProbeTec ET (Becton-Dickinson) assays. However, NAATs for 16S RNA (Aptima Combo 2, GenProbe), omp 1 (RealArt CT PCR, Artus and in-house NAATs) or the outer membrane complex B protein gene (omcB) established C trachomatis infection. Sequencing of omp 1 showed that the variant belonged to serovar I. PCR analysis indicated that the variant was plasmid free. The patient did not respond to single dose azithromycin treatment but subsequently responded to a course of doxycycline. Conclusions: A pathogenic plasmid free C trachomatis variant was identified. Clinicians should be alerted to the possibility of undetected C trachomatis infection caused by such variants and the potential of azithromycin failure in patients with recurrent chlamydial NGU. The occurrence of this variant is rare and should not form the basis for judgment of the performance or usefulness of plasmid based NAATs for C trachomatis detection.

Item Type: Article
Keywords: Adult, Anti-Bacterial Agents, administration & dosage, Azithromycin, administration & dosage, Chlamydia Infections, diagnosis, drug therapy, Chlamydia trachomatis, genetics, DNA, Bacterial, analysis, Humans, Male, Nucleic Acid Amplification Techniques, Polymerase Chain Reaction, Treatment Failure, Urethritis, microbiology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
PubMed ID: 17567684
Web of Science ID: 248433200021
URI: http://researchonline.lshtm.ac.uk/id/eprint/8512

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