Quantification of global transcription patterns in prokaryotes using spotted microarrays


Sidders, B; Withers, M; Kendall, SL; Bacon, J; Waddell, SJ; Hinds, J; Golby, P; Movahedzadeh, F; Cox, RA; Frita, R; Ten Bokum, AMC; Wernisch, L; Stoker, NG; (2007) Quantification of global transcription patterns in prokaryotes using spotted microarrays. Genome Biology, 8 (12). ISSN 1474-760X DOI: https://doi.org/10.1186/gb-2007-8-12-r265

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Abstract

We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Web of Science ID: 253451800018
URI: http://researchonline.lshtm.ac.uk/id/eprint/7843

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