Analysis of small GTPase function in trypanosomes


Field, MC; Horn, D; Carrington, M; (2008) Analysis of small GTPase function in trypanosomes. In: Balch, WE; Der, CJ; Hall, A, (eds.) Small GTPases in Disease, Part A. Elsevier Academic Press Inc, San Diego, pp. 57-76. ISBN 9780123739681 DOI: https://doi.org/10.1016/S0076-6879(07)38005-1

Full text not available from this repository.

Abstract

Trypanosomatids are protozoan parasites, of interest due to both their disease burden and deeply divergent position within the eukaryotic lineage. The African trypanosome, Trypanosoma brucei, has emerged as a very amenable model system, with a considerable toolbox of methods available, including inducible overexpression, RNA interference, and a completed genome. Here we describe some of the special considerations that need to be addressed when studying trypanosome gene function, and in particular small GTPases; we provide protocols for transfection, RNA interference, overexpression and basic transport assays, in addition to an overview of available vectors, cell lines, and strategies.

Item Type: Book Section
Keywords: VARIANT SURFACE GLYCOPROTEIN, BLOOD-STREAM FORMS, RNA INTERFERENCE, AFRICAN TRYPANOSOME, BRUCEI, TRAFFICKING, EXPRESSION, PROTEIN, SYSTEM, GENOME
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Web of Science ID: 255834100005
URI: http://researchonline.lshtm.ac.uk/id/eprint/7581

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
278Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item