Microarray analysis of the transcriptional responses of Clostridium difficile to environmental and antibiotic stress


Emerson, JE; Stabler, RA; Wren, BW; Fairweather, NF; (2008) Microarray analysis of the transcriptional responses of Clostridium difficile to environmental and antibiotic stress. Journal of medical microbiology, 57 (6). pp. 757-764. ISSN 0022-2615 DOI: 10.1099/jmm.0.47657-0

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Abstract

Clostridium difficile is a spore-forming anaerobic bacterium that is an emerging nosocomial threat; incidence of infection in hospitals is increasing, both in frequency and severity, resulting in considerable morbidity and mortality. In order to adapt to the intestinal environment, C. difficile must react to the many stresses involved with colonization, including exposure to antibiotics, which represents the most frequent precipitating agent of C. difficile infection. The responses of C. difficile to environmental shocks (heat, pH and oxidative shock) and to growth in the presence of subinhibitory concentrations of antibiotics (amoxicillin, clindamycin and metronidazole) were investigated using the C. difficile 630 microarray developed by the Bacterial Microarray Group at St George's, University of London, UK (B mu G@S). Significantly regulated genes and operons were identified that are unique to or common between different stresses. The transcriptional profiles of C. difficile 630 are similar after growth in the presence of amoxicillin and clindamycin: both increased transcription of ribosomal protein genes and altered transcription of genes encoding surface-associated proteins. In contrast, metronidazole treatment resulted in minor changes in transcription patterns. The general stress response is observed after heat shock and acid shock. Heat shock also affected transcription of several biochemical pathways. Exposure to atmospheric oxygen induced a large number of electron transporters. This study provides a starting point for detailed analyses of numerous genes whose expression is affected by stress and may therefore be involved in adaptation to the host environment.

Item Type: Article
Keywords: Anti-Bacterial Agents, pharmacology, Bacterial Proteins, genetics, metabolism, Clostridium difficile, drug effects, genetics, metabolism, Environment, Gene Expression Profiling, Gene Expression Regulation, Bacterial, drug effects, physiology, Heat, Hydrogen-Ion Concentration, Oxygen, pharmacology, Protein Array Analysis, Transcription, Genetic
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Antimicrobial Resistance Centre (AMR)
PubMed ID: 18480334
Web of Science ID: 256816900013
URI: http://researchonline.lshtm.ac.uk/id/eprint/7273

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