Differences between naive and memory T cell phenotype in Malawian and UK adolescents: a role for Cytomegalovirus?


Ben-Smith, A; Gorak-Stolinska, P; Floyd, S; Weir, RE; Lalor, MK; Mvula, H; Crampin, AC; Wallace, D; Beverley, PC; Fine, PE; Dockrell, HM; (2008) Differences between naive and memory T cell phenotype in Malawian and UK adolescents: a role for Cytomegalovirus? BMC Infect Dis, 8 (1). p. 139. ISSN 1471-2334 DOI: 10.1186/1471-2334-8-139

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Abstract

ABSTRACT: BACKGROUND: Differences in degree of environmental exposure to antigens in early life have been hypothesized to lead to differences in immune status in individuals from different populations, which may have implications for immune responses in later years. METHODS: Venous blood from HIV-negative adolescents and blood from the umbilical cords of babies, born to HIV-negative women, post-delivery was collected and analysed using flow cytometry. T cell phenotype was determined from peripheral blood lymphocytes and cytomegalovirus (CMV) seropositivity was assessed by ELISA in adolescents. RESULTS: HIV-negative Malawian adolescents were shown to have a lower percentage of naive T cells (CD45RO-CD62Lhi CD11alo), a higher proportion of memory T cells and a higher percentage of CD28- memory (CD28-CD45RO+) T cells compared to age-matched UK adolescents. Malawian adolescents also had a lower percentage of central memory (CD45RA-CCR7+) T cells and a higher percentage of stable memory (CD45RA+CCR7-) T cells than UK adolescents. All of the adolescents tested in Malawi were seropositive for CMV (59/59), compared to 21/58 (36%) of UK adolescents. CMV seropositivity in the UK was associated with a reduced percentage of naive T cells and an increased percentage of CD28- memory T cells in the periphery. No differences in the proportions of naive and memory T cell populations were observed in cord blood samples from the two sites. CONCLUSION: It is likely that these differences between Malawian and UK adolescents reflect a greater natural exposure to various infections, including CMV, in the African environment and may imply differences in the ability of these populations to induce and maintain immunological memory to vaccines and natural infections.

Item Type: Article
Faculty and Department: Academic Services & Administration > Academic Administration
Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: MEIRU
Population Studies Group
Tropical Epidemiology Group
PubMed ID: 18922182
Web of Science ID: 261610100001
URI: http://researchonline.lshtm.ac.uk/id/eprint/6756

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