Comparison of Cervicovaginal Lavage, Cervicovaginal Lavage Enriched With Cervical Swab, and Vaginal Tampon for the Detection of HIV-1 RNA and HSV-2 DNA in Genital Secretions


Delany, S; Rosas, R; Mlaba, N; Clayton, T; Akpomiemie, G; Legoff, J; Capovilla, A; Belec, L; Slevens, W; Mayaud, P; (2008) Comparison of Cervicovaginal Lavage, Cervicovaginal Lavage Enriched With Cervical Swab, and Vaginal Tampon for the Detection of HIV-1 RNA and HSV-2 DNA in Genital Secretions. Journal of acquired immune deficiency syndromes (1999), 49 (4). pp. 406-409. ISSN 1525-4135 DOI: https://doi.org/10.1097/QAI.0b013e31818c7f75

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Abstract

Methods: We compared the performance of 3 collection methods for cervicovaginal secretions [cervicovaginal lavage (CVL), CVL enriched with a cervical swab (eCVL), and vaginal tampon (VT)] to identify the most reliable method for detection of cervicovaginal HIV-1 and herpes simplex virus type 2 (HSV-2). HIV-1 RNA (Nuclisens EasyQ; BioMerieux, Marcy-I'Etoile. France), HSV-2 DNA (real-time polymerase chain reaction), and microscopic blood and semen traces were detected in samples from 19 HIV-1-HSV-2-coinfected women seen at 4 Weekly visits. Results: HIV-1 RNA was detected in 49 (79'%) of 62 eCVLs. 41 (61%) of 67 CVLs, and 27 (57%) of 47 VTs. Detection of HIV-1 RNA was higher in eCVL compared with CVL [45/58 (78%) vs. 32/58 (55%): risk ratio 1.41, 95'% confidence interval 1.05 to 1.88]. Conclusions: Although more eCVLs were contaminated with microscopic blood (29%) than CVLs (22%) or VTs (7%), detection of HIV-1 RNA retrained higher using eCVL compared with CVL (risk ratio 1.43, 95% confidence interval 1.02 to 2.02) in Uncontaminated samples. HSV-2 DNA was detected in less than 10%) of samples by each method but in 7 (37%) of 19 women overall by 1 or more methods.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Faculty of Epidemiology and Population Health > Dept of Medical Statistics
Research Centre: Centre for Maternal, Reproductive and Child Health (MARCH)
Centre for Global Non-Communicable Diseases (NCDs)
PubMed ID: 19186353
Web of Science ID: 260844300009
URI: http://researchonline.lshtm.ac.uk/id/eprint/6592

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