The nematode Panagrellus redivivus is susceptible to killing by human pathogens at 37 degrees C


Laws, TR; Smith, SA; Smith, MP; Harding, SV; Atkins, TP; Titball, RW; (2005) The nematode Panagrellus redivivus is susceptible to killing by human pathogens at 37 degrees C. FEMS microbiology letters, 250 (1). pp. 77-83. ISSN 0378-1097 DOI: 10.1016/j.femsle.2005.06.046

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Abstract

Caenorhabditis elegans has been used as a host for the study of bacteria that cause disease in mammals. However, a significant limitation of the model is that C elegans is not viable at 37 degrees C. We report that the gonochoristic nematode Panagrellus redivivus survives at 37 degrees C and maintains its life cycle at temperatures up to and including 31.5 degrees C. The C elegans pathogens Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, but not Yersinia pseudo tuberculosis, reduced P. redivivus lifespan. Of four strains of Burkholderia multivorans tested, one reduced P. redivivus lifespan at both temperatures, one was avirulent at both temperatures and two strains reduced P. redivivus lifespan only at 37 degrees C. The mechanism by which one of these strains killed P. redivivus at 37 degrees C, but not at 25 degrees C, was investigated further. Killing required viable bacteria, did not involve bacterial invasion of tissues, is unlikely to be due to a diffusible, bacterial toxin and was not associated with increased numbers of live bacteria within the intestine of the worm. We believe R multivorans may kill P. redivivus by a temperature-regulated mechanism similar to B. pseudomallei killing of C. elegans. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: nematode, bacterial pathogenicity, animal model, CAENORHABDITIS-ELEGANS, PSEUDOMONAS-AERUGINOSA, VIRULENCE FACTORS, MODEL HOST, SALMONELLA-TYPHIMURIUM, MOLECULAR-MECHANISMS, BURKHOLDERIA-CEPACIA, PARALYSIS, INFECTION, BACTERIA, Animals, Bacteria, isolation & purification, pathogenicity, ultrastructure, Burkholderia, pathogenicity, Caenorhabditis elegans, microbiology, Female, Humans, Longevity, Male, Microscopy, Electron, Pseudomonas aeruginosa, pathogenicity, Rhabditida, microbiology, ultrastructure, Salmonella enterica, pathogenicity, Species Specificity, Staphylococcus aureus, pathogenicity, Temperature, Virulence, Yersinia pseudotuberculosis, pathogenicity
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 16040202
Web of Science ID: 231403200011
URI: http://researchonline.lshtm.ac.uk/id/eprint/6530

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