CpG-DNA protects against a lethal orthopoxvirus infection in a murine model


Rees, DGC; Gates, AJ; Green, M; Eastaugh, L; Lukaszewski, RA; Griffin, KF; Krieg, AM; Titball, RW; (2005) CpG-DNA protects against a lethal orthopoxvirus infection in a murine model. Antiviral research, 65 (2). pp. 87-95. ISSN 0166-3542 DOI: 10.1016/j.antiviral.2004.10.004

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Abstract

CpG-DNA has been described as a potent activator of the innate immune system, with potential to protect against infection caused by a range of pathogens in a non-specific manner. Here two classes of CpG-DNA (CpG-A and CpG-B) have been investigated for their abilities to protect mice from infection with an orthopoxvirus (vaccinia virus). Dosing with either CpG-A or B by the intraperitonal or intranasal route protected mice against a subsequent intranasal challenge with vaccinia virus. To our knowledge, this is the first time CpG-mediated protection has been demonstrated. at the lung surface. The level of protection was greater when CpG-DNA was administered intranasally demonstrating a clear relationship between the route of CpG dosing and infection route. Treatment with CpG-B reduced viral titer in the lung by 10,000-fold at day 3 post-infection. The CC chemokines RANTES and MIP- Ibeta were elevated in the broncho-alveolar lavage from animals treated intranasally with CpG-B compared to untreated and intraperitoneally dosed controls, and it is possible that these chemokines play a role in the clearance of intranasally delivered vaccinia virus. Crown Copyright (C) 2004 Published by Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: CpG-DNA, orthopoxvirus, innate immunity, BACTERIAL-DNA, VACCINIA VIRUS, DEPENDENT PROTECTION, MICE, OLIGODEOXYNUCLEOTIDE, MOTIFS, GAMMA, CHALLENGE, INDUCTION, RESPONSES, Animals, Base Sequence, Chemokines, biosynthesis, Cytokines, biosynthesis, Disease Models, Animal, Female, Mice, Mice, Inbred BALB C, Oligodeoxyribonucleotides, genetics, pharmacology, Poxviridae Infections, immunology, prevention & control, virology, Vaccinia virus, immunology, pathogenicity, physiology, Virus Replication, drug effects
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 15708635
Web of Science ID: 227261900004
URI: http://researchonline.lshtm.ac.uk/id/eprint/6528

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