Combined high-resolution genotyping and geospatial analysis reveals modes of endemic urban typhoid fever transmission.


Baker, S; Holt, KE; Clements, AC; Karkey, A; Arjyal, A; Boni, MF; Dongol, S; Hammond, N; Koirala, S; Duy, PT; Nga, TV; Campbell, JI; Dolecek, C; Basnyat, B; Dougan, G; Farrar, JJ; (2011) Combined high-resolution genotyping and geospatial analysis reveals modes of endemic urban typhoid fever transmission. Open Biol, 1 (2). p. 110008. ISSN 2046-2441 DOI: https://doi.org/10.1098/rsob.110008

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Abstract

Typhoid is a systemic infection caused by Salmonella Typhi and Salmonella Paratyphi A, human-restricted bacteria that are transmitted faeco-orally. Salmonella Typhi and S. Paratyphi A are clonal, and their limited genetic diversity has precluded the identification of long-term transmission networks in areas with a high disease burden. To improve our understanding of typhoid transmission we have taken a novel approach, performing a longitudinal spatial case-control study for typhoid in Nepal, combining single-nucleotide polymorphism genotyping and case localization via global positioning. We show extensive clustering of typhoid occurring independent of population size and density. For the first time, we demonstrate an extensive range of genotypes existing within typhoid clusters, and even within individual households, including some resulting from clonal expansion. Furthermore, although the data provide evidence for direct human-to-human transmission, we demonstrate an overwhelming contribution of indirect transmission, potentially via contaminated water. Consistent with this, we detected S. Typhi and S. Paratyphi A in water supplies and found that typhoid was spatially associated with public water sources and low elevation. These findings have implications for typhoid-control strategies, and our innovative approach may be applied to other diseases caused by other monophyletic or emerging pathogens.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 22645647
Web of Science ID: 307107500001
URI: http://researchonline.lshtm.ac.uk/id/eprint/612552

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