Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes.


Beattie, T; Kaul, R; Rostron, T; Dong, T; Easterbrook, P; Jaoko, W; Kimani, J; Plummer, F; McMichael, A; Rowland-Jones, S; (2004) Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes. AIDS (London, England), 18 (11). pp. 1595-8. ISSN 0269-9370 DOI: https://doi.org/10.1097/01.aids.0000131362.82951.b2

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Abstract

: The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.

Item Type: Article
Faculty and Department: Faculty of Public Health and Policy > Dept of Global Health and Development
Research Centre: Social and Mathematical Epidemiology (SaME)
SaME Modelling & Economics
PubMed ID: 15238779
Web of Science ID: 222934500015
URI: http://researchonline.lshtm.ac.uk/id/eprint/55348

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