Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis


Champion, OL; Cooper, IAM; James, SL; Ford, D; Karlyshev, A; Wren, BW; Duffield, M; Oyston, PCF; Titball, RW; (2009) Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis. Microbiology (Reading, England), 155. pp. 1516-1522. ISSN 1350-0872 DOI: 10.1099/mic.0.026823-0

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Abstract

We report that larvae of the wax moth (Galleria mellonella) are susceptible to infection with the human enteropathogen Yersinia pseudotuberculosis at 37 degrees C. Confocal microscopy demonstrated that in the initial stages of infection the bacteria were taken up into haemocytes. To evaluate the utility of this model for screening Y. pseudotuberculosis mutants we constructed and tested a superoxide dismutase C (sodC) mutant. This mutant showed increased susceptibility to superoxide, a key mechanism of killing in insect haemocytes and mammalian phagocytes. It showed reduced virulence in the murine yersiniosis infection model and in contrast to the wildtype strain IP32953 was unable to kill G. mellonella. The complemented mutant regained all phenotypic properties associated with SodC, confirming the important role of this metalloenzyme in two Y. pseudotuberculosis infection models.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: TB Centre
PubMed ID: 19383703
Web of Science ID: 266151900014
URI: http://researchonline.lshtm.ac.uk/id/eprint/5315

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