PRKC-? Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention.


Yao, S; Bee, A; Brewer, D; Dodson, A; Beesley, C; Ke, Y; Ambroisine, L; Fisher, G; Møller, H; Dickinson, T; Gerard, P; Lian, LY; Risk, J; Lane, B; Smith, P; Reuter, V; Berney, D; Gosden, C; Scardino, P; Cuzick, J; Djamgoz, MB; Cooper, C; Foster, CS; (2010) PRKC-? Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention. Genes & cancer, 1 (5). pp. 444-64. ISSN 1947-6019 DOI: https://doi.org/10.1177/1947601910376079

Full text not available from this repository.

Abstract

: We show protein kinase C-zeta (PKC-?) to be a novel predictive biomarker for survival from prostate cancer (P < 0.001). We also confirm that transcription of the PRKC-? gene is crucial to the malignant phenotype of human prostate cancer. Following siRNA silencing of PRKC-? in PC3-M prostate cancer cells, stable transfectant cell line si-PRKC-?-PC3-M(T1-6) is phenotypically nonmalignant in vitro and in vivo. Genome-wide expression analysis identified 373 genes to be differentially expressed in the knockdown cells and 4 key gene networks to be significantly perturbed during phenotype modulation. Functional interconnection between some of the modulated genes is revealed, although these may be within different regulatory pathways, emphasizing the complexity of their mutual interdependence. Genes with altered expression following PRKC-? knockdown include HSPB1, RAD51, and ID1 that we have previously described to be critical in prostatic malignancy. Because expression of PRKC-? is functionally involved in promoting the malignant phenotype, we propose PKC-? as a novel and biologically relevant target for therapeutic intervention in prostate cancer.

Item Type: Article
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Non-Communicable Disease Epidemiology
PubMed ID: 21779455
URI: http://researchonline.lshtm.ac.uk/id/eprint/492452

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
260Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item