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Elena Deligianni, Natalie C. Silmon de Monerri, Paul J. McMillan, Lucia Bertuccini, Fabiana Superti, Maria Manola, Lefteris Spanos, Christos Louis, Michael J. Blackman, Leann Tilley, Inga Siden-Kiamos
Essential role of Plasmodium perforin-like protein 4 in ookinete midgut passage
www.plosone.org
www.plosone.org
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(8)Tj
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[()4()2()6( )-2()6()2()]TJ
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(.)Tj
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[()9()11()]TJ
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(a)Tj
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(of)Tj
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(~)Tj
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(1518)Tj
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(h)Tj
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(via)Tj
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(an)Tj
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(called)Tj
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(retort.)Tj
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(the)Tj
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(establishment)Tj
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(of)Tj
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(apical)Tj
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(polarity)Tj
3.3165 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(zygote)Tj
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([)Tj
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(13)Tj
0 g
(],)Tj
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(microtu-)Tj
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(bules)Tj
2.279 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4627 0 Td
(inner)Tj
2.3754 0 Td
(membrane)Tj
4.5638 0 Td
(complex)Tj
3.6169 0 Td
(\(IMC\))Tj
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(are)Tj
1.44 0 Td
(gradually)Tj
3.9231 0 Td
(elongated)Tj
4.0932 0 Td
(to)Tj
1.0205 0 Td
(finally)Tj
2.6985 0 Td
(form)Tj
2.1997 0 Td
(the)Tj
1.4683 0 Td
(cres-)Tj
-35.6652 -1.2982 Td
(cent)Tj
1.9105 0 Td
(shaped)Tj
3.016 0 Td
(ookinete.)Tj
3.9118 0 Td
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1.7858 0 Td
(newly)Tj
2.5739 0 Td
(formed)Tj
3.1578 0 Td
(ookinete)Tj
3.6906 0 Td
(rapidly)Tj
3.0388 0 Td
(traverse)Tj
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(the)Tj
1.4684 0 Td
(midgut)Tj
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(epithelium.)Tj
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(reaching)Tj
3.6793 0 Td
(the)Tj
1.4683 0 Td
(basal)Tj
2.2054 0 Td
(side,)Tj
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(the)Tj
1.4683 0 Td
(cell)Tj
1.5591 0 Td
(rounds)Tj
3.0671 0 Td
(up)Tj
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4.8472 0 Td
(the)Tj
1.474 0 Td
(basal)Tj
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(membrane)Tj
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(and)Tj
1.7291 0 Td
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(oocyst,)Tj
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(where)Tj
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(protein)Tj
3.1521 0 Td
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(formation)Tj
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(of)Tj
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(after)Tj
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(~10)Tj
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(days.)Tj
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(travel)Tj
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(gland,)Tj
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Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
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107.0929 0 0 23.6409 36 741.3165 cm
q
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Q
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Q
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34.0302 0 Td
(2)Tj
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Q
q
0.83 0.64 0.02 0 k
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1 J
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96.8315 707.6409 m
151.2 707.6409 l
h
f*
136.8567 696.6425 m
171.7228 696.6425 l
h
f*
36 685.6441 m
72.9071 685.6441 l
h
f*
90.9921 674.6457 m
176.8819 674.6457 l
h
f*
36 663.6472 m
47.2252 663.6472 l
h
f*
60.7748 652.6488 m
128.126 652.6488 l
h
f*
136.7433 641.6504 m
174.9543 641.6504 l
h
f*
36 630.652 m
65.3669 630.652 l
h
f*
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0.9496 0 Td
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1.7504 0 Td
(http://ww)Tj
3.4654 0 Td
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0.9497 0 Td
(CL,)Tj
0.83 0.64 0.02 0 k
1.4456 0 Td
(http://www)Tj
4.1103 0 Td
(.)Tj
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(evimalar.org)Tj
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0.9496 0 Td
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0.83 0.64 0.02 0 k
1.4457 0 Td
(http://www)Tj
4.1103 0 Td
(.transma)Tj
3.274 0 Td
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0.83 0.64 0.02 0 k
2.1472 0 Td
(https://)Tj
2.6079 0 Td
(www.mrc.ac.)Tj
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(gel)Tj
1.3549 0 Td
(was)Tj
1.7008 0 Td
(estimated.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 546.5763 cm
BT
/F15 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Generation)Tj
4.9417 0 Td
(of)Tj
1.0677 0 Td
(parasites)Tj
3.9261 0 Td
(expressing)Tj
4.611 0 Td
(PPLP4::mCherry)Tj
7.3229 0 Td
(fusion)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 529.6251 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(plasmid)Tj
3.3959 0 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(construct)Tj
3.9798 0 Td
(was)Tj
1.6951 0 Td
(pBAT-SIL6)Tj
4.7962 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.075 0 Td
(A)Tj
0.9014 0 Td
(2146)Tj
2.126 0 Td
(bp)Tj
1.2415 0 Td
(genomic)Tj
3.6737 0 Td
(fragment)Tj
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(was)Tj
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(produced)Tj
4.0421 0 Td
(by)Tj
1.1849 0 Td
(PCR)Tj
2.0636 0 Td
(using)Tj
2.3868 0 Td
(primers)Tj
3.3562 0 Td
(Per4_Fw_SacII)Tj
6.2645 0 Td
(and)Tj
1.7291 0 Td
(Per4_Rev_HpaI.)Tj
6.8258 0 Td
(The)Tj
1.7858 0 Td
(fragment)Tj
3.8665 0 Td
(was)Tj
-33.5053 -1.2982 Td
(inserted)Tj
3.4412 0 Td
(into)Tj
1.8368 0 Td
(the)Tj
1.474 0 Td
(plasmid)Tj
3.3902 0 Td
(after)Tj
2.0353 0 Td
(digestion)Tj
3.8835 0 Td
(with)Tj
2.0012 0 Td
(SacII)Tj
2.2337 0 Td
(and)Tj
1.7291 0 Td
(HpaI.)Tj
2.5058 0 Td
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2.812 0 Td
(transfection,)Tj
5.2157 0 Td
(the)Tj
1.4683 0 Td
(plasmid)Tj
-34.0268 -1.2983 Td
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1.6951 0 Td
(opened)Tj
3.1691 0 Td
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0.9524 0 Td
(the)Tj
1.4683 0 Td
(unique)Tj
3.0218 0 Td
(restriction)Tj
4.3596 0 Td
(site)Tj
1.5704 0 Td
(NcoI)Tj
2.228 0 Td
(to)Tj
1.0205 0 Td
(be)Tj
1.1508 0 Td
(inserted)Tj
3.4413 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
/F14 1 Tf
1.4683 0 Td
[()11()10()10()]TJ
/F9 1 Tf
2.3471 0 Td
(gene)Tj
2.0806 0 Td
(via)Tj
1.3776 0 Td
(a)Tj
0.652 0 Td
(single)Tj
-33.0404 -1.3039 Td
(crossover.)Tj
4.2235 0 Td
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5.3008 0 Td
(was)Tj
1.7008 0 Td
(performed)Tj
4.4673 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0536 0 Td
(above,)Tj
2.7722 0 Td
(and)Tj
1.7292 0 Td
(genotyping)Tj
4.7168 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(primers)Tj
-33.4429 -1.2983 Td
(5FRper4,)Tj
3.855 0 Td
(3Rper4)Tj
3.1068 0 Td
(and)Tj
1.7291 0 Td
(mcherryR)Tj
4.235 0 Td
(confirmed)Tj
4.3936 0 Td
(the)Tj
1.474 0 Td
(presence)Tj
3.7077 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(transgene,)Tj
4.32 0 Td
(while)Tj
2.3641 0 Td
(the)Tj
1.474 0 Td
(primer)Tj
-33.1481 -1.2982 Td
(pair)Tj
1.8084 0 Td
(5UTRFwper4/3UTRrevper)Tj
10.783 0 Td
(4)Tj
0.6916 0 Td
(was)Tj
1.7008 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0262 0 Td
(test)Tj
1.6044 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.4683 0 Td
(WT)Tj
1.7972 0 Td
(genomic)Tj
3.6736 0 Td
(architecture.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 420.7748 cm
BT
/F15 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Antibodies)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 403.8236 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(An)Tj
1.4513 0 Td
(antiserum)Tj
4.2689 0 Td
(recognizing)Tj
4.9209 0 Td
(PPLP4)Tj
2.914 0 Td
(was)Tj
1.6951 0 Td
(produced)Tj
4.0479 0 Td
(against)Tj
3.0274 0 Td
(the)Tj
/F14 1 Tf
1.4683 0 Td
[()]TJ
/F9 1 Tf
(.)Tj
/F14 1 Tf
0.9978 0 Td
[()9()11()4()1()10()4()16()-9()12()]TJ
/F9 1 Tf
4.473 0 Td
(protein.)Tj
3.3789 0 Td
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1.338 0 Td
(do)Tj
1.2529 0 Td
(this,)Tj
1.9048 0 Td
(a)Tj
-37.1392 -1.3039 Td
(fragment)Tj
3.8664 0 Td
(encoding)Tj
3.9344 0 Td
(amino)Tj
2.795 0 Td
(acid)Tj
1.8708 0 Td
(residues)Tj
3.481 0 Td
(150254)Tj
3.5943 0 Td
(was)Tj
1.6951 0 Td
(cloned)Tj
2.897 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
1.4683 0 Td
(vector)Tj
2.6986 0 Td
(pGEX6.1)Tj
3.8551 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
-35.7276 -1.2982 Td
(protein)Tj
3.1521 0 Td
(expressed)Tj
4.0932 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(GST)Tj
2.0239 0 Td
(and)Tj
1.7292 0 Td
(6xHis)Tj
2.5568 0 Td
(tagged)Tj
2.8346 0 Td
(version)Tj
3.1521 0 Td
(in)Tj
/F14 1 Tf
1.0375 0 Td
[(")]TJ
/F9 1 Tf
(.)Tj
/F14 1 Tf
0.9921 0 Td
[()7()10()12()]TJ
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1.5591 0 Td
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3.2371 0 Td
(cells.)Tj
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4.2406 0 Td
(protein)Tj
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(purified)Tj
3.4128 0 Td
(from)Tj
2.2054 0 Td
(inclusion)Tj
3.9174 0 Td
(bodies)Tj
2.812 0 Td
(solubilized)Tj
4.5127 0 Td
(by)Tj
1.1849 0 Td
(sonication)Tj
4.3823 0 Td
(in)Tj
1.0318 0 Td
(1.5%)Tj
2.1544 0 Td
(\(w/v\))Tj
2.3697 0 Td
(sarkosyl.)Tj
3.6737 0 Td
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1.7858 0 Td
(sec-)Tj
-35.138 -1.3039 Td
(ond)Tj
1.8028 0 Td
(antiserum)Tj
4.2689 0 Td
(directed)Tj
3.4866 0 Td
(against)Tj
3.0274 0 Td
(PPLP4)Tj
2.9083 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.1259 0 Td
(described)Tj
4.0479 0 Td
([)Tj
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(15)Tj
0 g
(].)Tj
2.0806 0 Td
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1.7801 0 Td
(13.1)Tj
1.8709 0 Td
(antibodies)Tj
4.3653 0 Td
(recogniz-)Tj
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(the)Tj
1.4683 0 Td
(Pbs21)Tj
2.5966 0 Td
(ookinete)Tj
3.6906 0 Td
(surface)Tj
3.0501 0 Td
(protein)Tj
3.1521 0 Td
(and)Tj
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(SOAP)Tj
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(have)Tj
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(been)Tj
2.126 0 Td
(described)Tj
4.0535 0 Td
([)Tj
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(22)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(23)Tj
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(].)Tj
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1.7858 0 Td
(antibody)Tj
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(against)Tj
3.0273 0 Td
(GAP50)Tj
3.1295 0 Td
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1.7007 0 Td
(a)Tj
0.652 0 Td
(kind)Tj
2.0579 0 Td
(gift)Tj
1.5421 0 Td
(from)Tj
2.2053 0 Td
(Dr)Tj
1.3266 0 Td
(Julian)Tj
2.5739 0 Td
(Rayner)Tj
3.0727 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
2.075 0 Td
(mCherry)Tj
3.855 0 Td
(was)Tj
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(detected)Tj
3.5716 0 Td
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(a)Tj
0.6519 0 Td
(rabbit)Tj
-35.1323 -1.304 Td
(polyclonal)Tj
4.3483 0 Td
(antibody)Tj
3.7644 0 Td
(from)Tj
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(Abcam.)Tj
3.3165 0 Td
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(antibodies)Tj
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(were)Tj
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(anti-mouse)Tj
4.7565 0 Td
(\(Alexa-488,)Tj
4.8188 0 Td
(Alexa-)Tj
-34.0778 -1.2982 Td
(555\))Tj
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(and)Tj
1.7291 0 Td
(anti-rabbit)Tj
4.5071 0 Td
(\(Alexa-488,)Tj
4.8132 0 Td
(Alexa-546\))Tj
4.5921 0 Td
(conjugated)Tj
4.6317 0 Td
(to)Tj
1.0262 0 Td
(Alexa)Tj
2.4718 0 Td
(Fluor)Tj
2.3981 0 Td
(\(Invitrogen\))Tj
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(or)Tj
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(anti-)Tj
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(rabbit)Tj
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(conjugated)Tj
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(to)Tj
1.0262 0 Td
(Cy-3)Tj
2.1656 0 Td
(\(Jackson)Tj
3.6567 0 Td
(Research\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 256.0251 cm
BT
/F15 1 Tf
1 TL
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9.0567 0 Td
(analysis)Tj
3.5056 0 Td
(\(IFA\))Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
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0 0 Td
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3.1691 0 Td
(ookinetes)Tj
4.0478 0 Td
(from)Tj
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(an)Tj
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(O/N)Tj
2.0353 0 Td
(culture)Tj
3.0387 0 Td
(were)Tj
2.1203 0 Td
(purified)Tj
3.4129 0 Td
(using)Tj
2.3868 0 Td
(NH)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 451.2755 237.3165 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 454.7905 239.0173 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Cl)Tj
1.1338 0 Td
(and)Tj
1.7291 0 Td
(then)Tj
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(placed)Tj
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(on)Tj
1.2755 0 Td
(poly-L-)Tj
-34.4236 -1.3039 Td
(lysine-coated)Tj
5.4822 0 Td
(covers)Tj
2.7609 0 Td
(slips)Tj
1.9729 0 Td
(before)Tj
2.7439 0 Td
(fixation)Tj
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(with)Tj
1.9955 0 Td
(4%)Tj
1.4514 0 Td
(paraformaldehyde)Tj
7.5286 0 Td
(\(PFA\))Tj
2.6703 0 Td
(in)Tj
1.0374 0 Td
(PBS)Tj
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(for)Tj
1.3833 0 Td
(15)Tj
1.1735 0 Td
(min.)Tj
-35.3307 -1.2982 Td
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1.7802 0 Td
(fixative)Tj
3.1181 0 Td
(was)Tj
1.7007 0 Td
(removed)Tj
3.7474 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(cells)Tj
1.9218 0 Td
(were)Tj
2.1203 0 Td
(permeabilized)Tj
5.8621 0 Td
(with)Tj
1.9955 0 Td
(0.2%)Tj
2.1543 0 Td
(saponin)Tj
3.4072 0 Td
(in)Tj
1.0318 0 Td
(PBS)Tj
1.8368 0 Td
(for)Tj
1.3833 0 Td
(3)Tj
-35.257 -1.2983 Td
(min.)Tj
2.0806 0 Td
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7.0072 0 Td
(with)Tj
1.9956 0 Td
(0.5%)Tj
2.1543 0 Td
(Triton)Tj
2.8233 0 Td
(X-100)Tj
2.6476 0 Td
(was)Tj
1.7007 0 Td
(also)Tj
1.7688 0 Td
(tested,)Tj
2.778 0 Td
(but)Tj
1.5533 0 Td
(no)Tj
1.2755 0 Td
(labelling)Tj
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(was)Tj
1.7008 0 Td
(detected)Tj
-33.0857 -1.3039 Td
(after)Tj
2.0353 0 Td
(this)Tj
1.6724 0 Td
(treatment.)Tj
4.3597 0 Td
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1.1111 0 Td
(the)Tj
1.4684 0 Td
(case)Tj
1.8595 0 Td
(of)Tj
1.0148 0 Td
(mCherry)Tj
3.8551 0 Td
(antibody)Tj
3.7644 0 Td
(permeabilization)Tj
6.9617 0 Td
(with)Tj
2.0013 0 Td
(0.5%)Tj
2.1543 0 Td
(saponin)Tj
3.4015 0 Td
(and)Tj
-35.6595 -1.2983 Td
(0.5%)Tj
2.1543 0 Td
(Triton)Tj
2.8233 0 Td
(gave)Tj
1.9956 0 Td
(satisfactory)Tj
4.7168 0 Td
(results.)Tj
3.0331 0 Td
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3.4979 0 Td
(antibodies)Tj
4.3653 0 Td
(were)Tj
2.1203 0 Td
(diluted)Tj
3.0387 0 Td
(in)Tj
1.0374 0 Td
(PBS)Tj
1.8312 0 Td
(with)Tj
2.0012 0 Td
(5%)Tj
1.4514 0 Td
(normal)Tj
-34.0665 -1.2982 Td
(goat)Tj
1.9219 0 Td
(serum.)Tj
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2.2847 0 Td
(incubation)Tj
4.5524 0 Td
(with)Tj
1.9956 0 Td
(secondary)Tj
4.269 0 Td
(antibodies)Tj
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(cells)Tj
1.9162 0 Td
(were)Tj
2.1259 0 Td
(mounted)Tj
3.8721 0 Td
(in)Tj
1.0374 0 Td
(Vectashield)Tj
-31.2885 -1.2983 Td
(\(Vector)Tj
3.2768 0 Td
(laboratories\).)Tj
-2.0806 -1.3039 Td
(Midgut)Tj
3.2032 0 Td
(ookinetes)Tj
4.0478 0 Td
(were)Tj
2.126 0 Td
(labeled)Tj
3.0274 0 Td
(according)Tj
4.1895 0 Td
(to)Tj
1.0205 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
2.0806 0 Td
(Briefly,)Tj
3.0728 0 Td
(mosquito)Tj
4.0194 0 Td
(midguts)Tj
3.4866 0 Td
(were)Tj
2.1203 0 Td
(dissected)Tj
-33.5903 -1.2983 Td
(24)Tj
1.1679 0 Td
(h)Tj
0.7483 0 Td
(after)Tj
2.0353 0 Td
(blood)Tj
2.5171 0 Td
(feeding,)Tj
3.3903 0 Td
(the)Tj
1.4683 0 Td
(blood)Tj
2.5115 0 Td
(bolus)Tj
2.3697 0 Td
(removed)Tj
3.7474 0 Td
(to)Tj
1.0261 0 Td
(PBS,)Tj
2.058 0 Td
(and)Tj
1.7348 0 Td
(a)Tj
0.6519 0 Td
(sample)Tj
3.0216 0 Td
(spotted)Tj
3.1521 0 Td
(on)Tj
1.2756 0 Td
(glass)Tj
2.0863 0 Td
(slides)Tj
-34.9622 -1.2983 Td
(and)Tj
1.7291 0 Td
(allowed)Tj
3.2882 0 Td
(to)Tj
1.0261 0 Td
(air)Tj
1.2926 0 Td
(dry)Tj
1.5704 0 Td
(followed)Tj
3.651 0 Td
(by)Tj
1.1849 0 Td
(fixation)Tj
3.2938 0 Td
(with)Tj
1.9956 0 Td
(methanol)Tj
4.0252 0 Td
(for)Tj
1.3889 0 Td
(10)Tj
1.1678 0 Td
(min)Tj
1.8595 0 Td
(at)Tj
0.9525 0 Td
(-80C.)Tj
2.8006 0 Td
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2.2847 0 Td
(blocking)Tj
-33.5109 -1.3039 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(presence)Tj
3.7077 0 Td
(of)Tj
1.0148 0 Td
(0.5%)Tj
2.1543 0 Td
(BSA,)Tj
2.1884 0 Td
(0.01%)Tj
2.6305 0 Td
(saponin)Tj
3.4016 0 Td
(the)Tj
1.4683 0 Td
(sample)Tj
3.0217 0 Td
(was)Tj
1.7008 0 Td
(incubated)Tj
4.1781 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(antiserum)Tj
4.2747 0 Td
(and)Tj
-35.7106 -1.2982 Td
(processed)Tj
4.1329 0 Td
(as)Tj
1.0148 0 Td
(above.)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 417.8834 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Function)Tj
3.9897 0 Td
(of)Tj
/F8 1 Tf
1.0418 0 Td
(Plasmodi)Tj
4.0394 0 Td
(um)Tj
/F0 1 Tf
1.5874 0 Td
(perforin-lik)Tj
4.5709 0 Td
(e)Tj
0.7654 0 Td
(protein)Tj
3.2173 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
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[()]TJ
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(.)Tj
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0.9978 0 Td
[()9()11()4()1()10()4()16()-9()12()]TJ
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4.473 0 Td
(PPLP4)Tj
2.914 0 Td
(\(PF3D7_0819400\),)Tj
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(acids)Tj
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(150254)Tj
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(1A)Tj
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(black\).)Tj
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2.9083 0 Td
(\()Tj
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(S3A)Tj
2.194 0 Td
(Fig)Tj
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(\).)Tj
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(S4A)Tj
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(S4B)Tj
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(24)Tj
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(the)Tj
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(\(3D-SIM\))Tj
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(15)Tj
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[()]TJ
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(.)Tj
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