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Fabiola Roman, Samanta das Chagas Xavier, Louisa A. Messenger, Márcio G. Pavan, Michael A. Miles, Ana María Jansen, Matthew Yeo
Dissecting the phyloepidemiology of Trypanosoma cruzi I (TcI) in Brazil by the use of high resolution genetic markers
www.plosntds.org
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(sylvatic)Tj
3.1635 0 Td
(environment,)Tj
5.6522 0 Td
(infecting)Tj
3.753 0 Td
(diverse)Tj
3.0501 0 Td
(host)Tj
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1.7291 0 Td
(vector)Tj
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(species)Tj
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(across)Tj
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(Americas)Tj
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(with)Tj
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(an)Tj
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(ancestral)Tj
3.7587 0 Td
(parental)Tj
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(origin)Tj
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(estimated)Tj
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(~0.50.9)Tj
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([)Tj
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(24)Tj
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(,)Tj
0.83 0.64 0.02 0 k
1.7404 0 Td
(23)Tj
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1.1055 0 Td
(Brazil,)Tj
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(is)Tj
0.8504 0 Td
(also)Tj
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(the)Tj
1.4684 0 Td
(most)Tj
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(widely)Tj
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(distributed)Tj
4.5978 0 Td
(DTU,)Tj
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(in)Tj
1.0375 0 Td
(terms)Tj
2.4945 0 Td
(of)Tj
1.0148 0 Td
(geography)Tj
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3.6453 0 Td
(of)Tj
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(host)Tj
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(and)Tj
1.7348 0 Td
(vector)Tj
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(species.)Tj
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(it)Tj
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(by)Tj
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(far,)Tj
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(the)Tj
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(most)Tj
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(25)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(30)Tj
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(].)Tj
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(et)Tj
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(al.)Tj
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([)Tj
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(31)Tj
0 g
(])Tj
1.8538 0 Td
(applied)Tj
3.1521 0 Td
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4.5184 0 Td
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5.6806 0 Td
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(\(MLMT\))Tj
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(the)Tj
-35.0926 -1.3039 Td
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(of)Tj
1.0148 0 Td
(TcI)Tj
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(population)Tj
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(substructure)Tj
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1.0318 0 Td
(samples)Tj
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(countries,)Tj
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(18)Tj
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(and)Tj
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(species,)Tj
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(across)Tj
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(48)Tj
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(tandem)Tj
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(repeats)Tj
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([)Tj
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(32)Tj
0 g
(].)Tj
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(revealed)Tj
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(extensive)Tj
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(intra)Tj
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(DTU)Tj
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(diversity)Tj
3.6453 0 Td
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(spatial)Tj
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(structuring)Tj
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(of)Tj
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(specific)Tj
3.1918 0 Td
(genotypes)Tj
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(associated)Tj
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(with)Tj
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(acute)Tj
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(oral)Tj
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(out-)Tj
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(breaks)Tj
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(or)Tj
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(vectorial)Tj
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(infections)Tj
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(in)Tj
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(Venezuela.)Tj
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(addition,)Tj
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(remarkable)Tj
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(genetic)Tj
3.0671 0 Td
(diversity,)Tj
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(through)Tj
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(multiclonality,)Tj
6.0264 0 Td
(was)Tj
1.7007 0 Td
(observed)Tj
3.7984 0 Td
(when)Tj
2.4038 0 Td
(a)Tj
0.652 0 Td
(single)Tj
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2.5341 0 Td
[()-4()4()4()15()10()10()18()-4()]TJ
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3.8835 0 Td
(reservoir)Tj
3.77 0 Td
(host)Tj
1.9162 0 Td
(of)Tj
1.0148 0 Td
(TcI)Tj
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1.7008 0 Td
(studied)Tj
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([)Tj
0.83 0.64 0.02 0 k
(33)Tj
0 g
(].)Tj
-32.9497 -1.2983 Td
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3.9231 0 Td
(to)Tj
1.0261 0 Td
(subdivide)Tj
4.0819 0 Td
(TcI)Tj
1.593 0 Td
(strains)Tj
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(into)Tj
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(groups)Tj
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(ongoing)Tj
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([)Tj
0.83 0.64 0.02 0 k
(34)Tj
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(et)Tj
0.9468 0 Td
(al.)Tj
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([)Tj
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(28)Tj
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(])Tj
1.8538 0 Td
(and)Tj
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(Cura)Tj
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(et)Tj
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(al.)Tj
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0.83 0.64 0.02 0 k
(35)Tj
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(])Tj
1.8539 0 Td
(described)Tj
4.0535 0 Td
(five)Tj
1.6498 0 Td
(haplotypes)Tj
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(associated)Tj
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(with)Tj
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(transmission)Tj
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(cycles)Tj
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(in)Tj
1.0262 0 Td
(Colombia,)Tj
4.3936 0 Td
(Chile)Tj
2.3414 0 Td
(and)Tj
1.7235 0 Td
(Bolivia.)Tj
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(Ramirez)Tj
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(et)Tj
0.9354 0 Td
(al.)Tj
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(36)Tj
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(used)Tj
2.0522 0 Td
(MLST)Tj
2.7326 0 Td
(to)Tj
1.0205 0 Td
(identify)Tj
3.2882 0 Td
(TcI)Tj
1.5873 0 Td
(genotypes)Tj
-33.5959 -1.2983 Td
(specifically)Tj
4.5694 0 Td
(associated)Tj
4.2576 0 Td
(with)Tj
1.9955 0 Td
(human)Tj
3.0785 0 Td
(infection)Tj
3.7927 0 Td
(\(TcI)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 394.1291 536.7118 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(DOM)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 411.874 538.4125 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
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(\))Tj
0.5612 0 Td
(and)Tj
1.7291 0 Td
(others)Tj
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1.9956 0 Td
(peridomes-)Tj
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(tic/sylvatic)Tj
4.4788 0 Td
(areas.)Tj
2.4661 0 Td
(MLST)Tj
2.7382 0 Td
(exploits)Tj
3.3052 0 Td
(nucleotide)Tj
4.4164 0 Td
(diversity)Tj
3.6453 0 Td
(present)Tj
3.1633 0 Td
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1.0318 0 Td
(four)Tj
1.9162 0 Td
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(ten)Tj
1.491 0 Td
(single-copy)Tj
4.7848 0 Td
(house-)Tj
-34.4633 -1.2982 Td
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(applied)Tj
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(to)Tj
1.0262 0 Td
(the)Tj
1.4682 0 Td
(study)Tj
2.3867 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F13 1 Tf
1.0375 0 Td
[()1()-9()]TJ
/F8 1 Tf
2.16 0 Td
(using)Tj
2.3868 0 Td
(different)Tj
3.6566 0 Td
(marker)Tj
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(combinations)Tj
5.709 0 Td
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(lineage)Tj
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(assignment)Tj
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(and)Tj
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(intraspecific)Tj
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(characterization)Tj
6.6614 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7405 0 Td
(37)Tj
0 g
(].)Tj
1.7348 0 Td
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3.8607 0 Td
(for)Tj
-35.7049 -1.2983 Td
(genetic)Tj
3.0671 0 Td
(exchange)Tj
3.9345 0 Td
(in)Tj
1.0318 0 Td
(TcI)Tj
1.5987 0 Td
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1.542 0 Td
(been)Tj
2.1203 0 Td
(reported,)Tj
3.8835 0 Td
(for)Tj
1.389 0 Td
(example,)Tj
3.7756 0 Td
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1.0318 0 Td
(strains)Tj
2.863 0 Td
(isolated)Tj
3.2881 0 Td
(from)Tj
/F13 1 Tf
2.2054 0 Td
[()-4()4()4()15()10()10()18()-4()]TJ
3.8834 0 Td
[(\r)6( )16()19()]TJ
-35.6142 -1.3039 Td
[()10()10()11()4( )11()12()-4()]TJ
/F8 1 Tf
3.1351 0 Td
(and)Tj
/F13 1 Tf
1.7348 0 Td
[()-4()6()4()12(\n)18()12()-4()]TJ
3.9288 0 Td
[()11()20()10()12()-4()]TJ
/F8 1 Tf
3.3449 0 Td
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1.0375 0 Td
(the)Tj
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(Amazon)Tj
3.6283 0 Td
(Basin)Tj
2.4095 0 Td
([)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
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1.8537 0 Td
(and)Tj
1.7291 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
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(domestic/peridomestic)Tj
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-35.3421 -1.2982 Td
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(in)Tj
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(Ecuador)Tj
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([)Tj
0.83 0.64 0.02 0 k
(39)Tj
0 g
(].)Tj
2.075 0 Td
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5.6069 0 Td
(generation)Tj
4.5069 0 Td
(of)Tj
1.0148 0 Td
(intra-lineage)Tj
5.2951 0 Td
(hybrids)Tj
3.2371 0 Td
(suggest)Tj
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(that)Tj
1.7745 0 Td
(TcI)Tj
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1.7688 0 Td
(displays)Tj
3.3846 0 Td
(a)Tj
0.6576 0 Td
(potential)Tj
3.7587 0 Td
(for)Tj
1.389 0 Td
(genetic)Tj
3.0671 0 Td
(exchange)Tj
3.9288 0 Td
([)Tj
0.83 0.64 0.02 0 k
(40)Tj
0 g
(].)Tj
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6.0434 0 Td
(DNA)Tj
2.3754 0 Td
(in)Tj
/F13 1 Tf
1.0318 0 Td
[()]TJ
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(.)Tj
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1.0375 0 Td
[()1()-9()]TJ
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2.16 0 Td
(has)Tj
1.542 0 Td
(a)Tj
0.6576 0 Td
(unique)Tj
3.0217 0 Td
(structure)Tj
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(and)Tj
1.7291 0 Td
(function)Tj
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(consisting)Tj
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(of)Tj
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(approxi-)Tj
-33.5279 -1.2983 Td
(mately)Tj
2.897 0 Td
(2050)Tj
2.6362 0 Td
(maxicircles)Tj
4.7225 0 Td
(\(~20kb\))Tj
3.4356 0 Td
(and)Tj
1.7291 0 Td
(thousands)Tj
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(smaller)Tj
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4.6374 0 Td
(\(~1.4kb\))Tj
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([)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
(].)Tj
2.0806 0 Td
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-34.2479 -1.2983 Td
(circle)Tj
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(DNA)Tj
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(inherited)Tj
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(and)Tj
1.7292 0 Td
(represents)Tj
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(a)Tj
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(in)Tj
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3.1975 0 Td
(DNA.)Tj
2.6022 0 Td
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4.49 0 Td
(et)Tj
0.941 0 Td
(al.)Tj
1.1225 0 Td
([)Tj
0.83 0.64 0.02 0 k
(41)Tj
0 g
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(developed)Tj
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(a)Tj
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(high)Tj
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(resolution)Tj
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4.3597 0 Td
(multilocus)Tj
4.4447 0 Td
(sequence)Tj
3.8608 0 Td
(typing)Tj
2.7666 0 Td
(\(mtMLST\))Tj
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(intra-DTU)Tj
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(multiple)Tj
3.5659 0 Td
(mitochondrial)Tj
5.9641 0 Td
(introgression)Tj
5.5162 0 Td
(events)Tj
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(and)Tj
1.7348 0 Td
(heteroplasmy)Tj
5.6012 0 Td
(within)Tj
2.812 0 Td
(South)Tj
-33.596 -1.2983 Td
(American)Tj
4.1896 0 Td
(TcI.)Tj
1.8199 0 Td
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5.5898 0 Td
(had)Tj
1.7122 0 Td
(already)Tj
3.1124 0 Td
(been)Tj
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(detected)Tj
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(America)Tj
3.6453 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(22)Tj
0 g
(])Tj
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(and)Tj
1.7291 0 Td
(in)Tj
1.0318 0 Td
(Bra-)Tj
-35.4611 -1.3039 Td
(zil)Tj
1.1452 0 Td
([)Tj
0.83 0.64 0.02 0 k
(42)Tj
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(])Tj
1.8539 0 Td
(and)Tj
1.7291 0 Td
(also)Tj
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(Bolivia)Tj
2.9764 0 Td
([)Tj
0.83 0.64 0.02 0 k
(43)Tj
0 g
(].)Tj
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3.8494 0 Td
(these)Tj
2.2563 0 Td
(studies)Tj
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(illustrate)Tj
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(several)Tj
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(remarkable)Tj
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(characteristics)Tj
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(of)Tj
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(TcI,)Tj
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(namely)Tj
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(the)Tj
-33.5506 -1.2983 Td
(immense)Tj
3.8835 0 Td
(geographic)Tj
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(distribution,)Tj
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(diversity)Tj
3.6453 0 Td
(of)Tj
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(host)Tj
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(and)Tj
1.729 0 Td
(vector)Tj
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(species,)Tj
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(extensive)Tj
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(diver-)Tj
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(sity,)Tj
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1.7292 0 Td
(the)Tj
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(capacity)Tj
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(exchange.)Tj
4.1556 0 Td
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4.0761 0 Td
(little)Tj
2.0012 0 Td
(is)Tj
0.8504 0 Td
(known)Tj
3.0047 0 Td
(about)Tj
2.4888 0 Td
(TcI)Tj
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(in)Tj
1.0318 0 Td
(Brazil)Tj
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(and)Tj
-34.6958 -1.2983 Td
(extraordinarily)Tj
6.2079 0 Td
(there)Tj
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(only)Tj
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(one)Tj
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(centric)Tj
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(publication)Tj
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(TcI)Tj
1.593 0 Td
([)Tj
0.83 0.64 0.02 0 k
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2.2166 0 Td
(scrubland)Tj
4.1669 0 Td
(\(savannah\))Tj
4.6432 0 Td
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3.6793 0 Td
(approximately)Tj
5.9754 0 Td
(2)Tj
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(mil-)Tj
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(lion)Tj
1.7858 0 Td
(km)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 231.0236 568.8566 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 236.8062 564.3779 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
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1.0147 0 Td
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3.2032 0 Td
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2.7609 0 Td
(comprising)Tj
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(23%)Tj
1.9276 0 Td
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1.0148 0 Td
(the)Tj
1.4683 0 Td
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2.0013 0 Td
(land)Tj
1.9785 0 Td
(surface)Tj
3.0501 0 Td
(area)Tj
1.8822 0 Td
([)Tj
0.83 0.64 0.02 0 k
(45)Tj
0 g
(].)Tj
2.0749 0 Td
(Scrubland)Tj
4.286 0 Td
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-35.1096 -1.2982 Td
(interspersed)Tj
5.1136 0 Td
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1.9955 0 Td
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2.829 0 Td
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4.2349 0 Td
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1.5534 0 Td
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4.6035 0 Td
(swamplands)Tj
5.1533 0 Td
(domi-)Tj
-34.4973 -1.2983 Td
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2.4491 0 Td
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1.1849 0 Td
[()28( )16()12()-9()14()7()4( )]TJ
3.7813 0 Td
[()-36()4()1()4()7()-3( )]TJ
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3.3902 0 Td
(palm)Tj
2.2394 0 Td
(trees)Tj
2.0976 0 Td
([)Tj
0.83 0.64 0.02 0 k
(46)Tj
0 g
(].)Tj
/F14 1 Tf
-13.9463 -1.3039 Td
(The)Tj
1.8482 0 Td
(Pantanal)Tj
/F8 1 Tf
3.9514 0 Td
(biome)Tj
2.7439 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.6519 0 Td
(large)Tj
2.16 0 Td
(seasonal)Tj
3.532 0 Td
(floodplain)Tj
4.3143 0 Td
(covering)Tj
3.6793 0 Td
(approximately)Tj
5.9754 0 Td
(140000)Tj
3.0728 0 Td
(km)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 552.9259 529.8519 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 558.7086 525.3732 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
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(at)Tj
-35.8693 -1.2982 Td
(the)Tj
1.4683 0 Td
(core)Tj
1.9446 0 Td
(of)Tj
1.0148 0 Td
(South)Tj
2.5568 0 Td
(America)Tj
3.6453 0 Td
([)Tj
0.83 0.64 0.02 0 k
(47)Tj
0 g
(].)Tj
2.075 0 Td
(It)Tj
0.856 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.652 0 Td
(biodiversity)Tj
4.9266 0 Td
(hotspot)Tj
3.2371 0 Td
(and)Tj
1.7291 0 Td
(freshwater)Tj
4.405 0 Td
(ecosystem)Tj
4.2917 0 Td
(of)Tj
1.0148 0 Td
(global)Tj
-34.6675 -1.2983 Td
(significance)Tj
4.9379 0 Td
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4.5241 0 Td
(diverse)Tj
3.05 0 Td
(mammal)Tj
3.7927 0 Td
(species)Tj
2.9991 0 Td
(and)Tj
1.7291 0 Td
(habitats.)Tj
3.5716 0 Td
(Climate)Tj
3.3732 0 Td
(instability)Tj
4.1669 0 Td
(results)Tj
2.812 0 Td
(in)Tj
-34.9566 -1.2982 Td
(periodic)Tj
3.5206 0 Td
(floods)Tj
2.6589 0 Td
(and)Tj
1.7291 0 Td
(droughts,)Tj
4.0308 0 Td
(affecting)Tj
3.6567 0 Td
(the)Tj
1.4683 0 Td
(population)Tj
4.5921 0 Td
(number)Tj
3.4186 0 Td
(and)Tj
1.7291 0 Td
(behavior)Tj
3.7134 0 Td
(of)Tj
1.0148 0 Td
(some)Tj
2.33 0 Td
(species)Tj
-33.8624 -1.304 Td
([)Tj
0.83 0.64 0.02 0 k
(48)Tj
0 g
(].)Tj
1.1962 -1.2982 Td
(The)Tj
/F14 1 Tf
1.7858 0 Td
(Amazon)Tj
/F8 1 Tf
3.753 0 Td
(biome)Tj
2.744 0 Td
(is)Tj
0.8447 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(largest)Tj
2.8176 0 Td
(hydrographic)Tj
5.6182 0 Td
(basin)Tj
2.3358 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(world,)Tj
2.7836 0 Td
(comprising)Tj
4.7679 0 Td
(44%)Tj
1.9275 0 Td
(of)Tj
-35.5632 -1.2983 Td
(the)Tj
1.4683 0 Td
(South)Tj
2.5568 0 Td
(American)Tj
4.1896 0 Td
(subcontinent.)Tj
5.709 0 Td
(The)Tj
1.7801 0 Td
(biome)Tj
2.7439 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.652 0 Td
(complex)Tj
3.6226 0 Td
(mosaic)Tj
3.0331 0 Td
(of)Tj
1.0148 0 Td
(very)Tj
1.9219 0 Td
(diverse)Tj
3.05 0 Td
(ecosystems,)Tj
-32.5925 -1.3039 Td
(dominated)Tj
4.5751 0 Td
(by)Tj
1.1792 0 Td
(tropical)Tj
3.2825 0 Td
(rain)Tj
1.8425 0 Td
(forests,)Tj
3.0557 0 Td
(with)Tj
1.9956 0 Td
(semi-arid)Tj
4.0422 0 Td
(regions,)Tj
3.3902 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.6576 0 Td
(variety)Tj
2.9197 0 Td
(of)Tj
1.0148 0 Td
(man-made)Tj
4.5808 0 Td
(land-)Tj
-34.265 -1.2983 Td
(scapes.)Tj
2.965 0 Td
(The)Tj
1.7801 0 Td
(Amazon)Tj
3.6284 0 Td
(biome)Tj
2.7439 0 Td
(contains)Tj
3.6056 0 Td
(the)Tj
1.474 0 Td
(greatest)Tj
3.2939 0 Td
(biological)Tj
4.0875 0 Td
(diversity)Tj
3.6453 0 Td
(\(in)Tj
1.3777 0 Td
(absolute)Tj
3.5262 0 Td
(terms\))Tj
2.829 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
-36.2321 -1.2982 Td
(planet)Tj
2.6872 0 Td
([)Tj
0.83 0.64 0.02 0 k
(49)Tj
0 g
(].)Tj
/F14 1 Tf
-1.491 -1.304 Td
(The)Tj
1.8482 0 Td
(Atlantic)Tj
3.6169 0 Td
(Forest)Tj
/F8 1 Tf
2.846 0 Td
(biome)Tj
2.7439 0 Td
(extends)Tj
3.2655 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.474 0 Td
(south)Tj
2.4377 0 Td
(of)Tj
1.0148 0 Td
(Pernambuco)Tj
5.3405 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(south)Tj
2.4434 0 Td
(of)Tj
1.0148 0 Td
(Rio)Tj
-33.9418 -1.2982 Td
(Grande)Tj
3.2371 0 Td
(do)Tj
1.2586 0 Td
(Sul,)Tj
1.6951 0 Td
(and)Tj
1.7291 0 Td
(it)Tj
0.7824 0 Td
(is)Tj
0.8504 0 Td
(characterized)Tj
5.5558 0 Td
(by)Tj
1.1849 0 Td
(humid)Tj
2.8913 0 Td
(tropical)Tj
3.2825 0 Td
(forest.)Tj
2.6929 0 Td
(This)Tj
1.9899 0 Td
(biome)Tj
2.744 0 Td
(is)Tj
0.8447 0 Td
(extensively)Tj
-30.7387 -1.2983 Td
(impacted)Tj
3.9288 0 Td
(by)Tj
1.1848 0 Td
(human)Tj
3.0728 0 Td
(activities.)Tj
3.9401 0 Td
(It)Tj
0.8617 0 Td
(originally)Tj
4.0252 0 Td
(encompassed)Tj
5.5672 0 Td
(12)Tj
1.1736 0 Td
(percent)Tj
3.2258 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(national)Tj
3.5036 0 Td
(territory)Tj
-32.9667 -1.2982 Td
(but)Tj
1.5533 0 Td
(only)Tj
1.9729 0 Td
(1)Tj
0.6974 0 Td
(to)Tj
1.0261 0 Td
(5)Tj
0.6973 0 Td
(percent)Tj
3.2258 0 Td
(\(less)Tj
1.9502 0 Td
(than)Tj
2.0296 0 Td
(100,000)Tj
3.2995 0 Td
(km)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 377.6881 360.8503 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 381.2598 356.4283 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\))Tj
0.5555 0 Td
(is)Tj
0.8504 0 Td
(intact)Tj
2.4888 0 Td
(today)Tj
2.4435 0 Td
([)Tj
0.83 0.64 0.02 0 k
(50)Tj
0 g
(].)Tj
2.0749 0 Td
(Containing)Tj
4.7679 0 Td
(more)Tj
2.3414 0 Td
(than)Tj
-33.647 -1.3039 Td
(8,000)Tj
2.3471 0 Td
(endemic)Tj
3.651 0 Td
(species,)Tj
3.2258 0 Td
(the)Tj
1.4684 0 Td
(Atlantic)Tj
3.4185 0 Td
(Forest)Tj
2.7099 0 Td
(is)Tj
0.8504 0 Td
(recognized)Tj
4.5863 0 Td
(as)Tj
1.0148 0 Td
(one)Tj
1.7008 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(world's)Tj
3.1351 0 Td
(most)Tj
2.2054 0 Td
(significant)Tj
-32.7966 -1.2982 Td
(biodiversity)Tj
4.9266 0 Td
(hotspots)Tj
3.5943 0 Td
([)Tj
0.83 0.64 0.02 0 k
(51)Tj
0 g
(].)Tj
/F14 1 Tf
-7.3246 -1.2983 Td
(The)Tj
1.8481 0 Td
(Caatinga)Tj
/F8 1 Tf
3.9628 0 Td
(biome)Tj
2.744 0 Td
(in)Tj
1.0374 0 Td
(northeast)Tj
3.9855 0 Td
(Brazil,)Tj
2.7609 0 Td
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4.2689 0 Td
(a)Tj
0.652 0 Td
(semi-arid)Tj
4.0421 0 Td
(ecological)Tj
4.1669 0 Td
(landscape)Tj
4.1329 0 Td
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-34.7978 -1.3039 Td
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1.9729 0 Td
(1%)Tj
1.457 0 Td
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1.1452 0 Td
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3.583 0 Td
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3.8834 0 Td
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4.4901 0 Td
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0.7822 0 Td
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4.4958 0 Td
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(agriculture)Tj
4.575 0 Td
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1.7348 0 Td
(cattle)Tj
2.3414 0 Td
(ranching)Tj
-33.5052 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(52)Tj
0 g
(].)Tj
2.075 0 Td
(This)Tj
1.9899 0 Td
(biome)Tj
2.7439 0 Td
(is)Tj
0.8447 0 Td
(characterized)Tj
5.5616 0 Td
(by)Tj
1.1792 0 Td
(clay)Tj
1.7801 0 Td
(and)Tj
1.7291 0 Td
(sandy)Tj
2.5454 0 Td
(soils)Tj
1.9616 0 Td
(with)Tj
1.9956 0 Td
(open)Tj
2.2167 0 Td
(plains)Tj
2.5965 0 Td
(supporting)Tj
4.6034 0 Td
(flora)Tj
2.0636 0 Td
(that)Tj
-35.8863 -1.2982 Td
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0.8448 0 Td
(typical)Tj
2.8629 0 Td
(of)Tj
1.0148 0 Td
(semi-arid)Tj
4.0422 0 Td
(regions)Tj
3.1635 0 Td
([)Tj
0.83 0.64 0.02 0 k
(53)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 242.9858 cm
BT
/F14 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Multilocus)Tj
4.7008 0 Td
(sequence)Tj
3.9638 0 Td
(typing)Tj
2.9055 0 Td
(\(MLST\))Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 211.9748 225.9779 cm
BT
/F14 1 Tf
1 TL
-0.004 Tc
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(MLST)Tj
2.7892 0 Td
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/F8 1 Tf
2.8233 0 Td
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1.7972 0 Td
(housekeeping)Tj
5.7032 0 Td
(gene)Tj
2.0807 0 Td
(fragments)Tj
4.2236 0 Td
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2.1203 0 Td
(initially)Tj
3.2428 0 Td
(considered)Tj
4.5977 0 Td
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1.9956 0 Td
(the)Tj
1.4627 0 Td
(objective)Tj
-34.0325 -1.2982 Td
(of)Tj
1.0148 0 Td
(detecting)Tj
3.8947 0 Td
(intra-lineage)Tj
5.2951 0 Td
(variation)Tj
3.8041 0 Td
(within)Tj
2.8119 0 Td
(TcI.)Tj
1.8198 0 Td
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4.4731 0 Td
(were)Tj
2.1203 0 Td
(selected)Tj
3.3449 0 Td
(from)Tj
2.211 0 Td
(Yeo)Tj
1.7801 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1282 0 Td
([)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(])Tj
-34.6391 -1.2983 Td
(and)Tj
1.7291 0 Td
(Diosque)Tj
3.5489 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1282 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(])Tj
1.8482 0 Td
(using)Tj
2.3924 0 Td
(the)Tj
1.4683 0 Td
(following)Tj
3.9799 0 Td
(rationale.)Tj
3.9741 0 Td
(Firstly,)Tj
2.9707 0 Td
(those)Tj
2.3414 0 Td
(known)Tj
3.0047 0 Td
(to)Tj
1.0205 0 Td
(be)Tj
1.1508 0 Td
(phylogeneti-)Tj
-31.4983 -1.3039 Td
(cally)Tj
2.0239 0 Td
(informative)Tj
4.8982 0 Td
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1.389 0 Td
(TcI)Tj
1.593 0 Td
(based)Tj
2.4718 0 Td
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4.0251 0 Td
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1.7348 0 Td
(discriminatory)Tj
6.1342 0 Td
(power)Tj
2.7269 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(37)Tj
0 g
(];)Tj
1.7348 0 Td
(sec-)Tj
-34.5144 -1.2983 Td
(ondly,)Tj
2.7268 0 Td
(reliable)Tj
3.1408 0 Td
(PCR)Tj
2.0636 0 Td
(amplification,)Tj
5.76 0 Td
(and)Tj
1.7348 0 Td
(thirdly)Tj
2.914 0 Td
(consistent)Tj
4.2519 0 Td
(sequencing)Tj
4.7168 0 Td
(quality)Tj
2.9537 0 Td
(in)Tj
1.0375 0 Td
(both)Tj
2.0579 0 Td
(5')Tj
0.9128 0 Td
(and)Tj
1.7291 0 Td
(3')Tj
-35.9997 -1.2982 Td
(directions.)Tj
4.439 0 Td
(Two)Tj
2.0239 0 Td
(genes)Tj
2.4378 0 Td
(previously)Tj
4.354 0 Td
(described)Tj
4.0535 0 Td
(by)Tj
1.1792 0 Td
(Yeo)Tj
1.7801 0 Td
(et)Tj
0.9468 0 Td
(al.)Tj
1.1225 0 Td
([)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(]:)Tj
2.075 0 Td
(mitochondrial)Tj
5.9697 0 Td
(peroxidase)Tj
-30.3815 -1.2983 Td
(\()Tj
/F13 1 Tf
[()97()]TJ
/F8 1 Tf
3.8947 0 Td
(and)Tj
1.7291 0 Td
(RNA-binding)Tj
5.743 0 Td
(protein-19)Tj
4.456 0 Td
(\()Tj
/F13 1 Tf
[( !)]TJ
/F8 1 Tf
(\))Tj
2.9877 0 Td
(and)Tj
1.7292 0 Td
(eight)Tj
2.2053 0 Td
(housekeeping)Tj
5.7089 0 Td
(genes)Tj
2.4378 0 Td
(from)Tj
2.2054 0 Td
(Lauthier)Tj
-33.0971 -1.3039 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1224 0 Td
([)Tj
0.83 0.64 0.02 0 k
(54)Tj
0 g
(]:)Tj
2.0807 0 Td
(glutathione)Tj
4.7621 0 Td
(peroxidase)Tj
4.5184 0 Td
(\()Tj
/F13 1 Tf
[(")]TJ
/F8 1 Tf
(\),)Tj
2.9764 0 Td
(3-hydroxy-3-methy)Tj
7.8406 0 Td
(lglutaril-CoA)Tj
5.5218 0 Td
(reductase)Tj
-29.7635 -1.2983 Td
(\()Tj
/F13 1 Tf
[(#)15()-2($)]TJ
/F8 1 Tf
(\),)Tj
5.0626 0 Td
(pyruvate)Tj
3.702 0 Td
(dehydrogenase)Tj
6.2135 0 Td
(component)Tj
4.8132 0 Td
(E1)Tj
1.2643 0 Td
(subunit)Tj
3.2598 0 Td
(alfa)Tj
1.6214 0 Td
(\()Tj
/F13 1 Tf
[()14()13(#)]TJ
/F8 1 Tf
(\),)Tj
3.1067 0 Td
(small)Tj
/F13 1 Tf
2.3244 0 Td
[(")]TJ
/F8 1 Tf
(-binding)Tj
-31.3679 -1.2982 Td
(protein)Tj
3.152 0 Td
(Rab7)Tj
2.2564 0 Td
(\()Tj
/F13 1 Tf
[(")]TJ
/F8 1 Tf
(\),)Tj
2.9424 0 Td
(Rho-like)Tj
/F13 1 Tf
3.6566 0 Td
[(")]TJ
/F8 1 Tf
(-binding)Tj
5.5162 0 Td
(protein)Tj
3.1521 0 Td
(\()Tj
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[(#)6( )]TJ
/F8 1 Tf
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