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Sarah E. Macdonald, Matthew J. Nolan, Kimberley Harman, Kay Boulton, David A. Hume, Fiona M. Tomley, Richard A. Stabler, Damer P. Blake
Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology
www.plosone.org
www.plosone.org
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(licence)Tj
2.9764 0 Td
(by)Tj
1.1848 0 Td
(cervical)Tj
3.2599 0 Td
(dislocation.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 591.2503 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Chicken)Tj
3.7842 0 Td
(management)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 574.2425 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(As)Tj
1.2642 0 Td
(part)Tj
1.8425 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(larger)Tj
2.5341 0 Td
(study)Tj
2.3868 0 Td
(250)Tj
1.6497 0 Td
(day-old,)Tj
3.4923 0 Td
(Cobb500)Tj
3.8267 0 Td
(broiler)Tj
2.914 0 Td
(chickens)Tj
3.6907 0 Td
(were)Tj
2.126 0 Td
(housed)Tj
3.0954 0 Td
(in)Tj
1.0318 0 Td
(coccidia-free)Tj
-31.521 -1.2982 Td
(conditions)Tj
4.473 0 Td
(at)Tj
0.9524 0 Td
(a)Tj
0.652 0 Td
(stocking)Tj
3.5886 0 Td
(density)Tj
3.1011 0 Td
(of)Tj
1.0148 0 Td
(34)Tj
1.1679 0 Td
(kg/m)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 370.6582 565.6818 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 376.3842 561.2598 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(anticipated)Tj
5.0002 0 Td
(end)Tj
1.7178 0 Td
(weight\).)Tj
3.4526 0 Td
(Following)Tj
4.2179 0 Td
(industry)Tj
3.5773 0 Td
(stan-)Tj
-35.6028 -1.2982 Td
(dard)Tj
2.0806 0 Td
(practice)Tj
3.3789 0 Td
(chickens)Tj
3.6907 0 Td
(were)Tj
2.1203 0 Td
(vaccinated)Tj
4.456 0 Td
(against)Tj
3.0273 0 Td
(infectious)Tj
4.1329 0 Td
(bronchitis)Tj
4.2973 0 Td
(on)Tj
1.2756 0 Td
(day)Tj
1.6384 0 Td
(of)Tj
1.0148 0 Td
(hatch)Tj
2.4321 0 Td
(\(using)Tj
-33.5449 -1.3039 Td
(Nobilis)Tj
3.1067 0 Td
(IB)Tj
1.1339 0 Td
(H120,)Tj
2.6362 0 Td
(MSD)Tj
2.3187 0 Td
(Animal)Tj
3.2145 0 Td
(Health,)Tj
3.1408 0 Td
(Milton)Tj
2.9762 0 Td
(Keynes,)Tj
3.3279 0 Td
(UK\).)Tj
2.1883 0 Td
(Throughout)Tj
5.0853 0 Td
(the)Tj
1.4684 0 Td
(study)Tj
2.3867 0 Td
(all)Tj
1.1395 0 Td
(chickens)Tj
-34.1231 -1.2983 Td
(had)Tj
1.7064 0 Td
(access)Tj
2.6476 0 Td
(to)Tj
1.0261 0 Td
(feed)Tj
1.8822 0 Td
(and)Tj
1.7291 0 Td
(water)Tj
/F13 1 Tf
2.4321 0 Td
[()6()12()14()11()14()7( )12()]TJ
/F7 1 Tf
(.)Tj
4.6092 0 Td
(Birds)Tj
2.3242 0 Td
(were)Tj
2.126 0 Td
(reared)Tj
2.7666 0 Td
(on)Tj
1.2756 0 Td
(a)Tj
0.6576 0 Td
(typical)Tj
2.8573 0 Td
(starter)Tj
2.778 0 Td
(diet)Tj
1.7404 0 Td
(supple-)Tj
-32.5584 -1.2983 Td
(mented)Tj
3.2598 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.474 0 Td
(anticoccidial)Tj
5.2837 0 Td
(Maxiban)Tj
/F15 1 Tf
(1)Tj
/F7 1 Tf
4.7679 0 Td
(\(Elanoco;)Tj
4.0194 0 Td
(Greenfield,)Tj
4.6828 0 Td
(Indiana,)Tj
3.5433 0 Td
(USA\))Tj
2.4548 0 Td
(until)Tj
2.1089 0 Td
(10)Tj
1.1736 0 Td
(days)Tj
1.9955 0 Td
(of)Tj
-36.7593 -1.2982 Td
(age,)Tj
1.7688 0 Td
(followed)Tj
3.6453 0 Td
(by)Tj
1.1849 0 Td
(anticoccidial-free)Tj
7.1433 0 Td
(`grower')Tj
3.4809 0 Td
(\(d11-24\))Tj
3.6849 0 Td
(and)Tj
1.7291 0 Td
(`finisher')Tj
3.7134 0 Td
(\(d25-29\))Tj
3.6793 0 Td
(diets)Tj
2.0977 0 Td
(\(Target)Tj
3.1634 0 Td
(Feeds)Tj
-35.291 -1.3039 Td
(Ltd,)Tj
1.8085 0 Td
(Shropshire,)Tj
4.7962 0 Td
(UK\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 455.4708 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Parasite)Tj
3.8456 0 Td
(propagation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 438.4629 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Sporulated)Tj
/F13 1 Tf
4.5467 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9921 0 Td
[()11()2()6()15()4()4()]TJ
/F7 1 Tf
2.7893 0 Td
(parasites)Tj
3.6907 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(Houghton)Tj
4.371 0 Td
(reference)Tj
3.9118 0 Td
(strain)Tj
2.5001 0 Td
(were)Tj
2.126 0 Td
(propagated)Tj
4.7168 0 Td
(and)Tj
1.7291 0 Td
(main-)Tj
-33.8567 -1.3039 Td
(tained)Tj
2.7155 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(previously)Tj
4.354 0 Td
([)Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(33)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 397.644 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Experimental)Tj
5.9858 0 Td
(design)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 380.6362 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(At)Tj
1.2018 0 Td
(nineteen)Tj
3.7021 0 Td
(days)Tj
1.9955 0 Td
(of)Tj
1.0148 0 Td
(age)Tj
1.5421 0 Td
(chickens)Tj
3.6907 0 Td
(were)Tj
2.1259 0 Td
(randomly)Tj
4.1272 0 Td
(assigned)Tj
3.6114 0 Td
(to)Tj
1.0204 0 Td
(either)Tj
2.5342 0 Td
(control)Tj
3.1237 0 Td
(or)Tj
1.0942 0 Td
(infected)Tj
3.4242 0 Td
(groups,)Tj
-34.2082 -1.2982 Td
(with)Tj
1.9955 0 Td
(each)Tj
2.0296 0 Td
(group)Tj
2.6079 0 Td
(housed)Tj
3.0954 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(separate)Tj
3.481 0 Td
(room)Tj
2.4264 0 Td
(to)Tj
1.0261 0 Td
(prevent)Tj
3.2542 0 Td
(accidental)Tj
4.2463 0 Td
(cross-infection.)Tj
6.3949 0 Td
(At)Tj
1.2019 0 Td
(21)Tj
1.1678 0 Td
(days)Tj
2.0013 0 Td
(of)Tj
-36.6177 -1.3039 Td
(age,)Tj
1.7688 0 Td
(25)Tj
1.1678 0 Td
(birds)Tj
2.2564 0 Td
(\(group)Tj
2.948 0 Td
(1\))Tj
1.0375 0 Td
(received)Tj
3.5319 0 Td
(a)Tj
0.652 0 Td
(single)Tj
2.5341 0 Td
(inoculum)Tj
4.0876 0 Td
(of)Tj
1.0148 0 Td
(1)Tj
0.6973 0 Td
(ml)Tj
1.2869 0 Td
(of)Tj
1.0148 0 Td
(DNase/RNase-free)Tj
7.6705 0 Td
(water,)Tj
2.6589 0 Td
(while)Tj
-34.3273 -1.2983 Td
(225)Tj
1.644 0 Td
(broilers)Tj
3.2769 0 Td
(in)Tj
1.0318 0 Td
(group)Tj
2.6135 0 Td
(2)Tj
0.6916 0 Td
(were)Tj
2.126 0 Td
(inoculated)Tj
4.422 0 Td
(with)Tj
1.9956 0 Td
(35,000)Tj
2.8233 0 Td
(sporulated)Tj
/F13 1 Tf
4.4334 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9977 0 Td
[()11()2()6()15()4()4()]TJ
/F7 1 Tf
2.7893 0 Td
(oocysts)Tj
3.1295 0 Td
(in)Tj
1.0374 0 Td
(1)Tj
0.6917 0 Td
(ml)Tj
1.2869 0 Td
(of)Tj
-34.9906 -1.2983 Td
(water.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 300.8125 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Sample)Tj
3.5716 0 Td
(collection)Tj
4.3276 0 Td
(and)Tj
1.8662 0 Td
(lesion)Tj
2.7921 0 Td
(scoring)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 283.8047 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Four)Tj
2.1543 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.652 0 Td
(half)Tj
1.7177 0 Td
(days)Tj
1.9956 0 Td
(\(108)Tj
1.9899 0 Td
(h\))Tj
1.0885 0 Td
(post)Tj
1.8992 0 Td
(infection)Tj
3.7928 0 Td
(all)Tj
1.1451 0 Td
(birds)Tj
2.2564 0 Td
(were)Tj
2.1203 0 Td
(culled)Tj
2.6192 0 Td
(\(26)Tj
1.508 0 Td
(days)Tj
1.9956 0 Td
(old\).)Tj
2.0693 0 Td
(Gender)Tj
3.2315 0 Td
(was)Tj
-33.9645 -1.2982 Td
(assigned)Tj
3.6056 0 Td
(at)Tj
0.9524 0 Td
(autopsy)Tj
3.3222 0 Td
(by)Tj
1.1792 0 Td
(identification)Tj
5.5842 0 Td
(of)Tj
1.0148 0 Td
(gonads.)Tj
3.2939 0 Td
(For)Tj
1.6214 0 Td
(this)Tj
1.6781 0 Td
(study)Tj
2.3867 0 Td
(caeca)Tj
2.3584 0 Td
(were)Tj
2.126 0 Td
(collected)Tj
3.7361 0 Td
(from)Tj
2.2053 0 Td
(49)Tj
-35.0643 -1.2983 Td
(female)Tj
2.8516 0 Td
(chickens)Tj
3.6907 0 Td
(and)Tj
1.7348 0 Td
(7)Tj
0.6916 0 Td
(male)Tj
2.143 0 Td
(chickens.)Tj
3.9174 0 Td
(Post-mortem,)Tj
5.76 0 Td
(caecal)Tj
2.6079 0 Td
(tissue)Tj
2.4548 0 Td
(was)Tj
1.7007 0 Td
(assessed)Tj
3.4753 0 Td
(immediately)Tj
5.1987 0 Td
(for)Tj
-36.2265 -1.3039 Td
(lesions)Tj
2.931 0 Td
(and)Tj
1.7347 0 Td
(scored)Tj
2.829 0 Td
(following)Tj
3.9798 0 Td
(the)Tj
1.474 0 Td
(method)Tj
3.3222 0 Td
(described)Tj
4.0535 0 Td
(by)Tj
1.1792 0 Td
(Johnson)Tj
3.549 0 Td
(and)Tj
1.7291 0 Td
(Reid)Tj
2.0636 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(])Tj
1.8482 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4683 0 Td
(same)Tj
-33.3465 -1.2983 Td
(experienced)Tj
5.0342 0 Td
(operator.)Tj
3.8722 0 Td
(Lesions)Tj
3.2258 0 Td
(were)Tj
2.1203 0 Td
(scored)Tj
2.8346 0 Td
(from)Tj
2.2053 0 Td
(0)Tj
0.6973 0 Td
(to)Tj
1.0205 0 Td
(4:)Tj
0.9241 0 Td
(0)Tj
0.6916 0 Td
(\(no)Tj
1.6158 0 Td
(lesions\),)Tj
3.4979 0 Td
(1)Tj
0.6973 0 Td
(\(mild)Tj
2.4208 0 Td
(lesions\),)Tj
3.4979 0 Td
(2)Tj
0.6917 0 Td
(\(mod-)Tj
-35.0473 -1.2982 Td
(erate)Tj
2.1713 0 Td
(lesions\),)Tj
3.4979 0 Td
(3)Tj
0.6973 0 Td
(\(severe)Tj
3.0161 0 Td
(lesions\),)Tj
3.4979 0 Td
(4)Tj
0.6973 0 Td
(\(very)Tj
2.2621 0 Td
(severe)Tj
2.6815 0 Td
(lesions\).)Tj
3.4979 0 Td
(Caecal)Tj
2.846 0 Td
(contents)Tj
3.6283 0 Td
(from)Tj
2.2054 0 Td
(one)Tj
1.7007 0 Td
(caeca)Tj
2.3585 0 Td
(per)Tj
-34.7582 -1.3039 Td
(bird)Tj
1.8935 0 Td
(was)Tj
1.6951 0 Td
(collected)Tj
3.7417 0 Td
(into)Tj
1.8425 0 Td
(a)Tj
0.652 0 Td
(sterile)Tj
2.6135 0 Td
(tube)Tj
1.9729 0 Td
(and)Tj
1.7291 0 Td
(immediately)Tj
5.2044 0 Td
(flash)Tj
2.0749 0 Td
(frozen)Tj
2.7723 0 Td
(in)Tj
1.0375 0 Td
(liquid)Tj
2.5625 0 Td
(nitrogen,)Tj
3.8721 0 Td
(including)Tj
-33.664 -1.2983 Td
(810)Tj
2.1656 0 Td
(birds)Tj
2.2507 0 Td
(per)Tj
1.5307 0 Td
(lesion)Tj
2.5738 0 Td
(score)Tj
2.3074 0 Td
(category.)Tj
3.8211 0 Td
(All)Tj
1.4003 0 Td
(samples)Tj
3.3789 0 Td
(were)Tj
2.126 0 Td
(stored)Tj
2.7099 0 Td
(at80C)Tj
3.8777 0 Td
(until)Tj
2.109 0 Td
(further)Tj
3.0274 0 Td
(process-)Tj
-33.2785 -1.2983 Td
(ing.)Tj
1.7234 0 Td
(All)Tj
1.4003 0 Td
(birds)Tj
2.2507 0 Td
(were)Tj
2.126 0 Td
(weighed)Tj
3.5376 0 Td
(two)Tj
1.7064 0 Td
(days)Tj
2.0013 0 Td
(before)Tj
2.7382 0 Td
(infection)Tj
3.7928 0 Td
(and)Tj
1.7291 0 Td
(immediately)Tj
5.1987 0 Td
(prior)Tj
2.2564 0 Td
(to)Tj
1.0261 0 Td
(culling.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 151.9937 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(DNA)Tj
2.3102 0 Td
(extraction)Tj
4.5071 0 Td
(and)Tj
1.8662 0 Td
(preparation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 134.9858 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(DNA)Tj
2.3754 0 Td
(was)Tj
1.7007 0 Td
(extracted)Tj
3.8835 0 Td
(from)Tj
2.2053 0 Td
(each)Tj
2.0296 0 Td
(sample)Tj
3.0217 0 Td
(of)Tj
1.0148 0 Td
(caecal)Tj
2.6022 0 Td
(contents)Tj
3.6283 0 Td
(using)Tj
2.3925 0 Td
(a)Tj
0.6519 0 Td
(QIAamp)Tj
3.7644 0 Td
(DNA)Tj
2.3754 0 Td
(Stool)Tj
2.2564 0 Td
(Kit)Tj
1.457 0 Td
(\(Qia-)Tj
-35.3591 -1.2982 Td
(gen,)Tj
1.8821 0 Td
(Hilden,)Tj
3.2258 0 Td
(Germany\),)Tj
4.5525 0 Td
(with)Tj
1.9955 0 Td
(the)Tj
1.474 0 Td
(following)Tj
3.9742 0 Td
(modifications.)Tj
5.9641 0 Td
(Briefly,)Tj
3.0727 0 Td
(following)Tj
3.9798 0 Td
(step)Tj
1.8198 0 Td
(2)Tj
0.6974 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-33.6527 -1.2983 Td
(QIAamp)Tj
3.7587 0 Td
(DNA)Tj
2.3811 0 Td
(Stool)Tj
2.2506 0 Td
(Kit)Tj
1.4627 0 Td
(protocol,)Tj
3.8211 0 Td
(twice)Tj
2.3131 0 Td
(the)Tj
1.474 0 Td
(sample)Tj
3.0217 0 Td
(volume)Tj
3.1974 0 Td
(of)Tj
1.0148 0 Td
(autoclaved)Tj
4.4957 0 Td
(Ballotini)Tj
3.617 0 Td
(beads)Tj
2.4718 0 Td
(\(0.4)Tj
-35.2797 -1.3039 Td
(0.6)Tj
1.3946 0 Td
(mm;)Tj
2.0863 0 Td
(VWR,)Tj
2.7269 0 Td
(Bristol,)Tj
3.0784 0 Td
(UK\))Tj
1.9672 0 Td
(were)Tj
2.1203 0 Td
(added)Tj
2.6646 0 Td
(and)Tj
1.7291 0 Td
(samples)Tj
3.3845 0 Td
(were)Tj
2.1203 0 Td
(homogenized)Tj
5.6523 0 Td
(for)Tj
1.3833 0 Td
(30)Tj
1.1735 0 Td
(seconds)Tj
3.3676 0 Td
(at)Tj
-34.8489 -1.2983 Td
(35,000)Tj
2.8232 0 Td
(oscillations/minute)Tj
7.903 0 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6576 0 Td
(Mini)Tj
2.194 0 Td
(Bead-beater)Tj
4.9946 0 Td
(24)Tj
1.1679 0 Td
(\(Bio-Spec,)Tj
4.3313 0 Td
(Bartlesville,)Tj
4.7962 0 Td
(USA\))Tj
2.4548 0 Td
(and)Tj
1.7348 0 Td
(chilled)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 401.7259 745.7952 cm
BT
/F6 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Eimeria)Tj
3.5504 0 Td
(tenella)Tj
/F0 1 Tf
3.1039 0 Td
(and)Tj
1.8567 0 Td
(the)Tj
1.5804 0 Td
(chicken)Tj
3.5504 0 Td
(caecal)Tj
3.0614 0 Td
(microbiom)Tj
4.5284 0 Td
(e)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
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2000 Adobe Systems Incorporated. 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