The diversity of Klebsiella pneumoniae surface polysaccharides.
Follador, R; Heinz, E; Wyres, KL; Ellington, MJ; Kowarik, M; Holt, KE; Thomson, NR; (2016) The diversity of Klebsiella pneumoniae surface polysaccharides. Microb Genom, 2 (8). e000073. ISSN 2057-5858 DOI: 10.1099/mgen.0.000073
Klebsiella pneumoniae is considered an urgent health concern due to the emergence of multi-drug-resistant strains for which vaccination offers a potential remedy. Vaccines based on surface polysaccharides are highly promising but need to address the high diversity of surface-exposed polysaccharides, synthesized as O-antigens (lipopolysaccharide, LPS) and K-antigens (capsule polysaccharide, CPS), present in K. pneumoniae. We present a comprehensive and clinically relevant study of the diversity of O- and K-antigen biosynthesis gene clusters across a global collection of over 500 K. pneumoniae whole-genome sequences and the seroepidemiology of human isolates from different infection types. Our study defines the genetic diversity of O- and K-antigen biosynthesis cluster sequences across this collection, identifying sequences for known serotypes as well as identifying novel LPS and CPS gene clusters found in circulating contemporary isolates. Serotypes O1, O2 and O3 were most prevalent in our sample set, accounting for approximately 80 % of all infections. In contrast, K serotypes showed an order of magnitude higher diversity and differ among infection types. In addition we investigated a potential association of O or K serotypes with phylogenetic lineage, infection type and the presence of known virulence genes. K1 and K2 serotypes, which are associated with hypervirulent K. pneumoniae, were associated with a higher abundance of virulence genes and more diverse O serotypes compared to other common K serotypes.
|Faculty and Department:||Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology|
|Research Centre:||Antimicrobial Resistance Centre (AMR)|
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Actions (login required)