Some synonymous and non-synonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-resistance results to fluoroquinolones with the Hain GenoType MTBDRsl assays.


Ajileye, A; Alvarez, N; Merker, M; Walker, TM; Akter, S; Brown, K; Moradigaravand, D; Schön, T; Andres, S; Schleusener, V; Omar, SV; Coll, F; Huang, H; Diel, R; Ismail, N; Parkhill, J; de Jong, BC; Peto, TE; Crook, DW; Niemann, S; Robledo, J; Smith, EG; Peacock, SJ; Köser, CU; (2017) Some synonymous and non-synonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-resistance results to fluoroquinolones with the Hain GenoType MTBDRsl assays. Antimicrobial agents and chemotherapy. ISSN 0066-4804 DOI: 10.1128/AAC.02169-16

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Abstract

We demonstrated that some non-synonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones using the Hain GenoType MTBDRsl assays (version 1 and 2) by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes, designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Antimicrobial Resistance Centre (AMR)
PubMed ID: 28137812
URI: http://researchonline.lshtm.ac.uk/id/eprint/3478630

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