Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis


Leonard, B; Coronel, J; Siedner, M; Grandjean, L; Caviedes, L; Navarro, P; Gilman, RH; Moore, DA; (2008) Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis. Journal of clinical microbiology, 46 (10). pp. 3526-9. ISSN 0095-1137 DOI: https://doi.org/10.1128/JCM.02083-07

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Abstract

The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).

Item Type: Article
Keywords: Antitubercular Agents/*pharmacology, Humans, Microbial Sensitivity Tests/methods, Mycobacterium tuberculosis/*drug effects, Oxazines/metabolism, Reproducibility of Results, Xanthenes/metabolism, Antitubercular Agents, pharmacology, Humans, Microbial Sensitivity Tests, methods, Mycobacterium tuberculosis, drug effects, Oxazines, metabolism, Reproducibility of Results, Xanthenes, metabolism
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Research Centre: TB Centre
PubMed ID: 18701659
Web of Science ID: 259758900060
URI: http://researchonline.lshtm.ac.uk/id/eprint/3338

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