Comparative genome analysis and global phylogeny of the toxin variant Clostridium difficile PCR Ribotype 017 reveals the evolution of two independent sub-lineages.


Cairns, MD; Preston, MD; Hall, CL; Gerding, DN; Hawkey, PM; Kato, H; Kim, H; Kuijper, EJ; Lawley, TD; Pituch, H; Reid, S; Kullin, B; Riley, TV; Solomon, K; Tsai, PJ; Weese, JS; Stabler, RA; Wren, BW; (2016) Comparative genome analysis and global phylogeny of the toxin variant Clostridium difficile PCR Ribotype 017 reveals the evolution of two independent sub-lineages. Journal of clinical microbiology. ISSN 0095-1137 DOI: https://doi.org/10.1128/JCM.01296-16

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Abstract

: The diarrhoeal pathogen Clostridium difficile consists of at least six distinct evolutionary lineages. The RT017 lineage is anomalous as strains only express toxin B, compared to strains from other lineages that produce toxins A and B and occasionally binary toxin. Historically, RT017 were initially reported in Asia but have now been reported worldwide. We used whole genome sequencing and phylogenetic analysis to investigate the patterns of global spread and population structure of 277 RT017 isolates from animal and human origins from six continents, isolated between 1990 and 2013. We reveal two distinct evenly split sub-lineages (SL1 and SL2) of C. difficile RT017 that contain multiple independent clonal expansions. All 24 animal isolates were contained within SL1 along with human isolates suggesting potential transmission between animals and humans. Genetic analyses revealed an over representation of antibiotic resistance genes. Phylogeographic analyses show a North American origin for RT017 as has been found for the recently emerged epidemic RT027 lineage. Despite only having one toxin, RT017 strains have evolved in parallel from at least two independent sources and can readily transmit between continents.<br/>

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 28031436
Web of Science ID: 397595300024
URI: http://researchonline.lshtm.ac.uk/id/eprint/3309546

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