Making standards for quantitative real-time pneumococcal PCR.


Morpeth, SC; Huggett, JF; Murdoch, DR; Scott, JA; (2014) Making standards for quantitative real-time pneumococcal PCR. Biomol Detect Quantif, 2. pp. 1-3. ISSN 1472-6963 DOI: 10.1016/j.bdq.2014.11.003

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Abstract

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.

Item Type: Article
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
PubMed ID: 27896138
URI: http://researchonline.lshtm.ac.uk/id/eprint/3187736

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