Making standards for quantitative real-time pneumococcal PCR.

Morpeth, SC; Huggett, JF; Murdoch, DR; Scott, JA; (2014) Making standards for quantitative real-time pneumococcal PCR. Biomol Detect Quantif, 2. pp. 1-3. ISSN 1472-6963 DOI:

[img] Text - Published Version

Download (263kB)


Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.

Item Type: Article
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
PubMed ID: 27896138


Download activity - last 12 months
Downloads since deposit
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item