Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models.


O'Sullivan, DM; Sander, C; Shorten, RJ; Gillespie, SH; Hill, AV; McHugh, TD; McShane, H; Tchilian, EZ; (2007) Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models. Vaccine, 25 (49). pp. 8203-5. ISSN 0264-410X DOI: https://doi.org/10.1016/j.vaccine.2007.09.065

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Abstract

Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: TB Centre
PubMed ID: 17980937
Web of Science ID: 251809400001
URI: http://researchonline.lshtm.ac.uk/id/eprint/3137

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