Blastocystis mitochondrial genomes appear to show multiple independent gains and losses of start and stop codons.


Jacob, AS; Andersen, LO; Bitar, PP; Richards, VP; Shah, S; Stanhope, MJ; Stensvold, CR; Clark, CG; (2016) Blastocystis mitochondrial genomes appear to show multiple independent gains and losses of start and stop codons. Genome Biol Evol, 8 (11). pp. 3340-3350. ISSN 1759-6653 DOI: https://doi.org/10.1093/gbe/evw255

[img]
Preview
Text - Published Version
License:

Download (838kB) | Preview

Abstract

: Complete mitochondrion-related organelle (MRO) genomes of several subtypes (STs) of the unicellular stramenopile Blastocystis are presented. Complete conservation of gene content and synteny in gene order is observed across all MRO genomes, comprising 27 protein coding genes, 2 ribosomal RNA genes, and 16 transfer RNA (tRNA) genes. Despite the synteny, differences in the degree of overlap between genes were observed between subtypes and also between isolates within the same subtype. Other notable features include unusual base-pairing mismatches in the predicted secondary structures of some tRNAs. Intriguingly, the rps4 gene in some MRO genomes is missing a start codon and, based on phylogenetic relationships among STs, this loss has happened twice independently. One unidentified open reading frame (orf160) is present in all MRO genomes. However, with the exception of ST4 where the feature has been lost secondarily, orf160 contains variously one or two in-frame stop codons. The overall evidence suggests that both the orf160 and rps4 genes are functional in all STs, but how they are expressed remains unclear.<br/>

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases
Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 27811175
Web of Science ID: 393815300008
URI: http://researchonline.lshtm.ac.uk/id/eprint/3070195

Statistics


Download activity - last 12 months
Downloads since deposit
28Downloads
50Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item