Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood.


Xu, G; Nolder, D; Reboud, J; Oguike, MC; van Schalkwyk, DA; Sutherland, CJ; Cooper, JM; (2016) Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood. Angewandte Chemie (International ed in English). ISSN 1433-7851 DOI: https://doi.org/10.1002/anie.201606060

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Abstract

We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper-folding technique of origami to enable the sequential steps of DNA extraction, loop-mediated isothermal amplification (LAMP), and array-based fluorescence detection. A low-cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a "sample-to-answer" diagnosis from a finger-prick volume of human blood, within 45 min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80 patient samples benchmarked against the gold-standard polymerase chain reaction (PCR) assay in an operator-blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Faculty of Infectious and Tropical Diseases
Research Centre: Malaria Centre
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PubMed ID: 27554333
Web of Science ID: 389224000008
URI: http://researchonline.lshtm.ac.uk/id/eprint/2830510

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