The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications.


Thi Khanh Nhu, N; Riordan, DW; Do Hoang Nhu, T; Thanh, DP; Thwaites, G; Huong Lan, NP; Wren, BW; Baker, S; Stabler, RA; (2016) The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications. Sci Rep, 6. p. 28291. ISSN 2045-2322 DOI: 10.1038/srep28291

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Abstract

Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the drug of last resort for many infections. In this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible Vietnamese isolates and one susceptible reference strain (MIC <1.5 μg/μl). Whole genome sequencing was used to identify single nucleotide mutations that could be attributed to the reduced colistin susceptibility. We identified six lpxACD and three pmrB mutations, the majority of which were novel. In addition, we identified further mutations in six A. baumannii genes (vacJ, pldA, ttg2C, pheS and conserved hypothetical protein) that we hypothesise have a role in reduced colistin susceptibility. This study has identified additional mutations that may be associated with colistin resistance through novel resistance mechanisms. Our work further demonstrates how rapidly A. baumannii can generate resistance to a last resort antimicrobial and highlights the need for improved surveillance to identified A. baumannii with an extensive drug resistance profile.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Antimicrobial Resistance Centre (AMR)
PubMed ID: 27329501
Web of Science ID: 378221700001
URI: http://researchonline.lshtm.ac.uk/id/eprint/2572263

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